Team:NWU-China/Characterization
Data measurement
We overexpressed pigment protein-fwYellow
（BBa_K1033910）、spisPink（BBa_K1033932）、gfasPurple（BBa_K1033919）
in E. coli and extracted and purified. The maximum absorbance was measured in a 96-well plate using a microplate reader (Synergy 2).
Fig.1 spectral scan results
At the same time, we found that fwYellow emits strong fluorescence under blue light excitation.
Fig.2 under blue light excitation, The left one is fwYellow
When we used the microplate reader to measure the excitation light and absorbed light, we found that the spectrum of fwYellow is too narrow. Due to the low precision of the instrument, it is impossible to accurately measure its excitation light and emission light. The excitation light is around 522 nm and the emission is about 536 nm.