Team:JNFLS/Notebook
Notebook
Week1 (4.8-4.14)
★Had the first meeting.
★Brainstorm and learning about relevant materials.
★Discuss our ideas and invited teachers to give some advice
Week2 (4.22-4.28)
★Presentation to share what we learn about synthetic biology.
★Second Brainstorm.
★We began to use our spare time to study experimental techniques.
★project was decided.
Week3 (5.12-5.11)
★We had a safety education before we started the formal experiment, including lab safety, researcher safety and environment safety. And we learned some emergency responses, such as how to use extinguisher and fire blanket, and how to use emergency shower and eye wash.
★Made a concrete plan for our project.
Week4 (5.22-5.26)
★Extracting of plasmid.
★Constructing recombinant plasmid
★Learning cell culture techniques
Week5 (5.27-6.2)
★The construction of recombinant plasmid.
★We have decided to build our parts and primers sequence was designed
★Transformation, monoclonal selected, we have sent plasmid to the company for sequencing verification
Week6 (6.3-6.9)
★After connecting the cleavage products, we have sent them to the company for sequencing.
★We started building two parts using PCR technique and digestion technique:
★According to our design, We should know the sensitivity of the promoter. So we decided to construct two plasmids.
Week7 (6.10-6.16)
★successfully constructed and cryopreserved.
★Continuing to build part.
★We went to Jinan Hing School to have teaching activities for publicity our project and Synthetic Biology.
★Preparing of experimental materials.
Week8 (6.17-6.23)
★Construct part K3209000.
★Characterization K1334002
★designed a series of questionnaires.
★Data analysis.
Week9 (6.24-6.30)
★It was verified by sequencing that we have building parts K3209000 successfully.
★Through a small range of issuing questionnaires, we have further modified our questionnaires.
★Continuing to construct other part, and preparing the materials for the second plasmid vectors.
Week10 (7.1-7.7)
★Extracting plasmids and began to detect promoter sensitivity to formaldehyde.
★Exprssion theK3209000
★The construction the part K3209002.
★We started issuing questionnaires in a wide range.
Week11 (7.8-7.14)
★Continuing to Construct the Part K3209003.
★SDS-PAGE analysis for exprssed protein K3209000.
★It was verified by sequencing that we have constructed the first vector successfully. And we began to constructed other part K3209006.
Week12 (7.15-7.21)
★Continue to build part K3209004.
★Continue to expression protein, and purification.
★we have carried out preliminary experiments to be familiar with the use of instruments.
★Continue to construct the part.K3209006.
Week13 (7.22-7.28)
★Expression and purification of expressed protein.
★It was verified by sequencing that we have building K3209002 successfully.
★we added a series of IPTG with a concentration gradient to some of the tubes with the same initial fluorescence intensity and measured the change in its fluorescence intensity.
Week14 (7.29-8.4)
★Do HP work, publicity.
★The plasmid vector was finally constructed successfully after hard working, and we decided to transfect the plasmid into the cells.
★It was verified by sequencing that we have constructed the secong vector successfully.
Week15 (8.5-8.11)
★Detected the sensitivity of promoter to fromaldehyde (repeat).
★We have made of poster for CCiC.
★After data processing, we got the graph which showed the influence of IPTG on the GFP expression.
Week16 (8.12-8.18)
★Characterization the part: K3209006.
★Expression and purifation of protein (SDS-PAGE).
★Public propaganda of science in the Technology Museum.
★We transformed these two plasmids to competent cells, and according to their different resistances, we selected the strain of interest.
Week17 (8.19-8.25)
★We take part in the CCiC in Shenzhen.
★We went to science museum to spread the idea of iGEM and hand out questionnaires.
Week18 (8.26-9.1)
★SDS-PAGE for expressed the BFD-M7 and TalB-F187Y.
★Characterization part K1334002
Week19 (9.2-9.8)
★We have launched a series of activities to promote the IGEM competition for Freshmen.
★A presentation for the students in our own school
Week20 (9.9-9.15)
★Purification fo expressed protein.
★Characterization part K2890001
★HPLC analysis for products.
★Data analysis.
Week21 (9.16-9.22)
★Repeat Purification fo expressed protein.
★Characterization part K2890001
★Repeat HPLC analysis for products.
★Data analysis.
Week22 (9.23-9.29)
★Modify the condition of purification protein.
★Repeat HPLC analysis.
★Data analysis.
Week23 (9.30-10.6)
★Characterization of K3209006.
★We have processed the data of questionnaire survey.
★After many measurements and data processing, we have realized the sensitivity of promoter to formaldehyde.
Week24 (10.7-10.13)
★Start wiki work.
★Data analysis.
★Art work for wiki.
Week25 (10.14-10.20)
★Data analysis.
★Continue wiki work.
★Prepared for Poster and presentation.