Team:JNFLS/Demonstrate
Demonstrate
Our project not only remove formaldehyde indoors, but also generate valuable xylulose with formaldehyde. That kills two birds with one stone!
First, we had constructed a real hardware device that can collect formaldehyde indoors, by which formaldehyde could be removed from indoor. The structure, function, using method and other details are described in “Design” part.
The metabolic pathway of formaldehyde, generating xylulose, have been constructed and expressed two enzymes (BFD-M7 and TalB-F187Y) with activities. They can catalyze the glycolaldehyde and dihydroxyacetone (DHA) productions, as well as xylulose. These results had been proved with HPLC analysis, showed in the following figures.
Figure 1. HPLC analysis of the products catalyzed by BFD-M7.
A: Standard glycolaldehyde; B: Standard DHA; C: The products catalyzed by BFD-M7 in vitro. The results showed that our expression vector expressed active BFD-M7, which catalyzed the synthesis of glycolaldehyde and DHA.
Figure 2. HPLC analysis of the xylulose product catalyzed by TalB-F187Y.
A: Standard Xylulose; B: The xylulose product catalyzed by TalB-F187Y in vitro. The results showed that our expression vector expressed active TalB-F187Y, which catalyzed the synthesis of xylulose from glycolaldehyde and DHA.
Considering the hardware with the lab work, we believe that our system would work in the realistic conditions.