Team:IIT-Madras/Parts

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PARTS

BBa_K3163001

Arabidopsis thaliana strictosidine synthase (STR)

Strictodine synthase (STR) is a plant enzyme that condenses tryptamine and secologanin to produce strictosidine. This part consists of the coding sequence of the STR gene.

BBa_K3163002

eGFP with NOS terminator driven by a CaMV 35s promoter

This part consists of the fluorescent reporter eGFP driven by the Cauliflower Mosaic Virus 35s promoter commonly used in plants. The NOS terminator is used to terminate transcription.

We have validated this part as part of the silver medal criteria and this can be found here.

BBa_K3163003

STR with CaMV 35s terminator

Strictosidine synthase gene is driven by a CaMV 35s promoter and CaMV 35s terminator. It expresses the enzyme strictosidine synthase which condenses tryptamine and secologanin to yield strictosidine.

BBa_K3163004

pCAMBIA 1301 plasmid

pCAMBIA 1301 is a vector used for genetic engineering of plants.

We modified our cloning strategy to a consecutive cloning approach. We planned to clone in GFP into pCAMBIA, validate this part and then clone in STR into this modified plasmid.