As the mRNA transcript data of eforRed chromoprotein is generated in the system with a varying DSF by wet lab team, we calculated the difference in mRNA transcription level between two systems.

Objectives:

(1) To characterize the efficiency of promoter PspA

Result:

Figure 1: Raw data obtained from rt-qPCR by wet lab team, and result after process.

By calculating the reverse log2 of each data, which indicated the cycles of the proliferation of mRNA transcript in the system, the fold difference between two promotors are presented and plotted by Prism.

Figure 2: Fold change of PspA promoter compared with lac-T7 promoter.

The above figure shows the fold change difference of CBS-pspA promoter activity normalized to lac-T7 activity. According to the above figure, we find that the activity of the CBS-pspA promoter only achieves about 11.7% - 37.5% of the original activity of lac-T7. The lower activity of the CBS-pspA promoter may contribute to the absence of a red signal from our device. For our further improvement, we advise that the dry lab modeling should calculate the copy number of the promoter and the strength of ribosomal binding site that are required to compensate to achieve the original activity of lac-T7 and the visible red signal.

Team:Hong Kong-CUHK/Promoter Activity

Promotor Activity

Overview:

Objectives:

Result: