Before building our design, we modeled our system with Matlab calculation and SimBiology Model Analyzer. Our dry lab data shows that our system works under 67-100 uM DSF treatment without taking account of the influence of sigma54 and RNA polymerase collision. Our latter experimental data proved that this simulation is valid.

To further examine our design, we also attempted to use this model to calculate the activity difference between T7 promoter and pspA promoter.

Through this calculation, we gave constructive contributions to our knowledge of our system.

Please refer to Dry lab: Promoter activity and Dry lab: Testification section for more detail.

Team:Hong Kong-CUHK/Model

Modeling