Time flies when you’re having fun, and what is more fun than doing something that no one has ever done before? Our laboratory work revolved around examining protein secretion via the twin-arginine translocation (Tat) pathway. We did a huge number of experiments over the course of our iGEM project, from basic cloning and PCR to fractionation and expression tests, and managed to demonstrate that our idea of using the Tat pathway of Vibrio natriegens is an option for protein translocation purposes. Our model showcases what could possibly be achieved later on with the VibXPresso strain. However, a lot of work still needs to be completed, and this years iGEM has only worked as a starting block for future endeavours. VibXPresso is coming, and it’s fast!
In the lab part of our Wiki, we have included the experiments we conducted during the summer, and a summary of results we achieved. Protocols and a timeline in lab notebook can be found as separate pdf files: Protocols and Lab Notebook.