Team:TUDelft/Parts
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Our Part Collection﹀
Engineering non-model organisms requires discovery of organism-specific parts or re-tuning of parts characterized in model organisms. Our part collection provides modular cloning (MoClo) (Webber et al., 2018) compatible parts that can be used for predictable expression of genes across different bacterial species.
Our collection provides essential parts to overcome the need to identify parts for different bacterial hosts and insulate the parts from variations associated with microbial context.
Identification of origins of replication for different bacterial species is laborious. Although broad host range plasmids can be used across a wide range of bacterial species, they work in specific groups of microorganisms (Jain and Srivastava., 2013). Our toolkit provides all the components (four proteins and two origins of replication) of the Phi29 replication machinery necessary to establish host independent (orthogonal) replication. The advantage of establishing orthogonal replication, is little or no knowledge of bacterial host is needed and higher control of the system can be established.
Non-model bacterial species have a limited collection of promoters (Yang et al., 2018) . Our toolkit provides T7 promoter variants of different strengths which in combination with (portable expression systems) , can be used for gene expression across different bacterial species. In addition, the collection consists of a broad host range promoter (PBHR) based on conserved regions of -10 region and -35 region sequences in E.coli and B.subtilis <a href="#Yang"> (Yang et al., 2018) </a> .
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<img class="parts" src="
" alt="ribozyme">
To achieve modularity and predictability of synthetic parts, insulation from their genetic context needs to be established. Sequences downstream of the transcription start site (TSS) of promoters end up in transcripts and are shown to significantly affect translational rates <a href="#Lou"> (Lou et al., 2012) </a>. Our toolkit consists of ribozymes which when included in 5’UTR sequences, due to their self cleavage properties, cleave any artifacts left behind in the transcripts upstream of the RBS.
<img class="parts" src="
" alt="RBS">
Our toolkit contains an RBS (universal RBS) predicted to work using <a href="http://www.denovodna.com"> RBS calculator 2.0 </a> across a range of bacterial species. We have demonstrated the working of the universal RBS in E.coli and P.putida.
<img class="parts" src="
" alt="CDS">
Codon usage across bacterial species varies. Our toolkit provides a cross-species <a href="2019.igem.org/Team:TUDelft/Model">codon harmonization tool</a>. The tool can be used to generate coding sequences with equal codon usage across a range of organisms.
<img class="parts" src="
" alt="terminator">
Our toolkit consists of variants of T7 terminators. Use of these variants reduces chances of homologous recombination between transcriptional units <a href="#Temme"> (Temme et al., 2012) </a>.
<img class="parts" src="
" alt="TALE">
Behavior of parts is influenced by microbial context dependent variations (eg. copy number, transcription rates and translation rates) and to overcome the need to re-characterize parts for different bacterial species, an incoherent feed forward loop (iFFL) can be implemented in a genetic circuit <a href="#Segall-Shapiro"> (Segall-Shapiro et al., 2018) </a>. To establish an iFFL genetic circuit, TALE (Transcriptional Activator Like Effector) proteins can be used as repressors. Our toolkit contains the gene to express TALE protein which is designed to target a specific DNA sequence. Furthermore, we engineered a series of promoters that can be targeted by the TALE protein.
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Part Collection
<thead>
♥ Name Type Description ♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918034">BBa_K2918034</a> Coding Φ29 DNA polymerase (DNAP/p2) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53810"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918001">BBa_K2918001</a> Coding Φ29 Terminal Protein (TP/p3) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53857"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918002">BBa_K2918002</a> Coding Φ29 Single Stranded Binding Protein (SSB/p5) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918003">BBa_K2918003</a> Coding Φ29 Double Strand Binding Protein (DSB/p6) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58056"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918033">BBa_K2918033</a> Other Φ29 Left origin of replication (Ori-L) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55150"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918061">BBa_K2918061</a> Other Φ29 Right origin of replication (Ori-R) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="57586"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918029">BBa_K2918029</a> Composite OriL-GFP-Kan-OriR <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53814"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918005">BBa_K2918005</a> Regulatory Weak T7 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53859"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918006">BBa_K2918006</a> Regulatory medium T7 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54344"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918009">BBa_K2918009</a> Regulatory medium T7sp1 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54263"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918010">BBa_K2918010</a> Regulatory T7sp1 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58623"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">♥ <a target="_blank"class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918054">BBa_K2918054</a> Regulatory PBHR sp1 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53815"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918016">BBa_K2918016</a> Other T7 terminator variant <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53815"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918014">BBa_K2918014</a> RBS Universal RBS <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55140"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918012">BBa_K2918012</a> Other Ribo J <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58055"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918039">BBa_K2918039</a> Other SarJ <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54379"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918013">BBa_K2918013</a> RBS BBa_B0032 RBS and SarJ
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54383"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">
<a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918035">BBa_K2918035</a> RBS BBa_B0032 RBS and RiboJ <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54374"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918036">BBa_K2918036</a> RBS Universal RBS and SarJ <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54375"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918038">BBa_K2918038</a> RBS Universal RBS and RiboJ <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53815"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918008">BBa_K2918008</a> Coding TALEsp1 <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58618"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918050">BBa_K2918050</a> Composite Cross-species based iFFL <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58061"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">
♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918040">BBa_K2918040</a> Composite T7 promoter based optimized iFFL <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58615"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link"></a> <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918046">BBa_K2918046</a> Composite T7 promoter based iFFL <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58616"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918048">BBa_K2918048</a> Composite 0.5 T7 promoter based iFFL </tbody>
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- <a id="bestpart" class="toggle" href="javascript:void(0);" >Our Favorite Basic Part﹀</a>
Our Favorite basic part is <a target="_blank" href="http://parts.igem.org/Part:BBa_K2918054">PBHRsp1 promoter, BBa_K2918054</a>. We successfully demonstrated its function in Escherichia coli and Pseudomonas putida. Furthermore, it contains a binding site for TALE (Transcriptional Activator like Effector) repressor protein, facilitating its implementation in incoherent feed forward loop genetic circuits. Hence, this promoter can be used for controllable gene expression across different bacterial species.
<figure style="margin-left:auto;margin-right:auto;"><img style="border-radius:4%;" class="banneroh" src = "
" alt="Putida Plate">
<figcaption>Figure 1:The functioning of this part has been confirmed qualitatively in P. putida</figcaption></figure> - <a id="bestcomposite" class="toggle" href="javascript:void(0);" >Our Favorite Composite Part﹀</a>
Our favorite composite part is <a target="_blank" href="http://parts.igem.org/Part:BBa_K2918040">BBa_K2918040, a T7 promoter based optimized iFFL</a>. This part has been demonstrated to insulate gene expression from variations in T7 RNAP concentrations and promoter strengths. Furthermore, it is compatible with portable T7 RNAP expression systems. Thereby, setting the basis for the development of universal expression systems.
- <a id="allparts" class="toggle" href="javascript:void(0);" >All parts﹀</a>
<thead>
♥ Name Type Description
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53809"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">
<a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918000">BBa_K2918000</a> Regulatory PBHR <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53810"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918001">BBa_K2918001</a> Coding Φ29 Terminal Protein (TP/p3) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53857"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918002">BBa_K2918002</a> Coding Φ29 Single Stranded Binding Protein (SSB/p5) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918003">BBa_K2918003</a> Coding Φ29 Double Strand Binding Protein (DSB/p6) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53814"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918005">BBa_K2918005</a> Regulatory Weak T7 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53859"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918006">BBa_K2918006</a> Regulatory medium T7 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53816"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918007">BBa_K2918007</a> Regulatory Wild type T7 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54257"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918008">BBa_K2918008</a> Coding Transcription Activator like Effector protein (TALEsp1) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54344"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918009">BBa_K2918009</a> Regulatory medium T7sp1 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54263"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918010">BBa_K2918010</a> Regulatory T7sp1 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54349"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918011">BBa_K2918011</a> Regulatory PBHR sp1 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55140"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918012">BBa_K2918012</a> Other Ribo J <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54379"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918013">BBa_K2918013</a> RBS BBa_B0032 RBS and SarJ <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53815"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918014">BBa_K2918014</a> RBS Universal RBS <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53929"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918015">BBa_K2918015</a> Terminator Wild type T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54402"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918016">BBa_K2918016</a> Terminator T7 terminator variant <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54076"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918017">BBa_K2918017</a> Composite 0.1 T7 promoter - Universal RBS - Φ29 9 TP - WT T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54191"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918018">BBa_K2918018</a> Composite 0.5 T7 promoter - Universal RBS - Φ29 TP - T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54192"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918019">BBa_K2918019</a> Composite WT T7 promoter - Universal RBS - Φ29 TP - T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54193"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918020">BBa_K2918020</a> Composite 0.1 T7 promoter - Universal RBS - Φ29 DNAP - T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54194"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918021">BBa_K2918021</a> Composite 0.5 T7 promoter - Universal RBS - Φ29 DNAP - T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53926"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918022">BBa_K2918022</a> Composite WT T7 promoter - Universal RBS - Φ29 DNAP - WT T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53813"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918023">BBa_K2918023</a> Composite Weak T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53927"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918024">BBa_K2918024</a> Composite Medium T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53928"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918025">BBa_K2918025</a> Composite WT T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53930"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918026">BBa_K2918026</a> Composite 0.1 T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53931"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918027">BBa_K2918027</a> Composite 0.5 T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53932"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918028">BBa_K2918028</a> Composite WT T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="57586"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918029">BBa_K2918029</a> Composite OriL-GFP-Kan-OriR <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54195"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918030">BBa_K2918030</a> Composite WT T7 promoter - Universal RBS - GFP - T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54196"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918031">BBa_K2918031</a> Composite 0.1 T7 promoter - Universal RBS - GFP - T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54198"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918032">BBa_K2918032</a> Composite 0.5 T7 promoter - Universal RBS - GFP - T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58056"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918033">BBa_K2918033</a> Other Φ29 Left origin of replication (Ori-L) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54351"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918034">BBa_K2918034</a> Coding Φ29 DNA polymerase (DNAP/p2) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54383"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918035">BBa_K2918035</a> RBS BBa_B0032 RBS and RiboJ <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54374"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918036">BBa_K2918036</a> RBS SarJ and Universal RBS <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54352"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918037">BBa_K2918037</a> Coding Cross-species harmonized eGFP <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54375"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918038">BBa_K2918038</a> RBS RiboJ and Universal RBS <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58055"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918039">BBa_K2918039</a> Other SarJ <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form> <input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58061">
<input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918040">BBa_K2918040</a> Composite T7 promoter based optimized iFFL <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form> <input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58572"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918041">BBa_K2918041</a> Coding Kanamycin resistance gene <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form> <input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58626"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918043">BBa_K2918043</a> Composite T7sp1-RiboJ-UniRBS-hGFP-T7 terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58627"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918044">BBa_K2918044</a> Composite T7sp1-RiboJUniRBS-jGFP-T7terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58628"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918045">BBa_K2918045</a> Composite Pbhrsp1-RiboJUniRBS-jGFP-T7terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58615"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918046">BBa_K2918046</a> Composite T7 promoter based iFFL <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58763"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918047">BBa_K2918047</a> Regulatory low T7sp1 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58616"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918048">BBa_K2918048</a> Composite 0.5 T7 promoter based iFFL <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58629"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918049">BBa_K2918049</a> Composite Pbhrsp1_v2, RiboJ_URBS_jGFP_T7terminator <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58618"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918050">BBa_K2918050</a> Composite Cross-species based iFFL <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58630"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918051">BBa_K2918051</a> Composite Pbhr_sp2, RiboJURBS, jGFP,T7 <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58584"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918052">BBa_K2918052</a> Coding eGFP <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58613"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918053">BBa_K2918053</a> Regulatory T7 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58623"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft">♥ <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918054">BBa_K2918054</a> Regulatory PBHR sp1 promoter <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="58624"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918055">BBa_K2918055</a> Regulatory PBHR promoter sp2 <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55150"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918061">BBa_K2918061</a> Other Φ29 Right origin of replication (Ori-R) <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55155"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918062">BBa_K2918062</a> Composite Universal RBS (adapted for modular cloning with ribozymes) </tbody></div> </div> </div> </li> </ul>
References
- <a id="Jain" href="https://academic.oup.com/femsle/article/348/2/87/731695" target="_blank"> Jain, A. and P. Srivastava (2013). "Broad host range plasmids." FEMS Microbiology Letters 348(2): 87-96. </a>
- <a id="Lou" href="http://www.ncbi.nlm.nih.gov/pubmed/23034349" target="_blank"> Lou, C., Stanton, B., Chen, Y., Munsky, B., & Voigt, C. (2012). Ribozyme-based insulator parts buffer synthetic circuits from genetic context. Nature Biotechnology, 30(11), 1137-1142. doi: 10.1038/nbt.2401 </a>
- <a id="Segall-Shapiro" href="http://doi.org/10.1038/nbt.4111" target="_blank"> Segall-Shapiro, T. H., et al. (2018). "Engineered promoters enable constant gene expression at any plasmid copy number in bacteria." Nature Biotechnology 36: 352. </a>
<a id="Temme" href="https://academic.oup.com/nar/article/40/17/8773/2411560" target="_blank"> Temme, K., Hill, R., Segall-Shapiro, T., Moser, F., & Voigt, C. (2012). Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research, 40(17), 8773-8781. doi: 10.1093/nar/gks597 </a>
- <a id="Yang" href="http://www.ncbi.nlm.nih.gov/pubmed/29061047" target="_blank"> Yang, S., et al. (2018). "Construction and Characterization of Broad-Spectrum Promoters for Synthetic Biology." ACS Synthetic Biology 7(1): 287-291. </a>
- <a id="Webber" href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0016765" target="_blank">Weber, E., Engler, C., Gruetzner, R., Werner, S., & Marillonnet, S. (2011). A Modular Cloning System for Standardized Assembly of Multigene Constructs. Plos ONE, 6(2), e16765. </a>
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