During our Experiments we used abreviations to simplify the results annotation. MN = Microneedle FD = Flavescence Dorée BN = Bois Noir EC = Endogeneous Control
DNA Extraction
To test our method of extraction, we decided to try it on a non-infected grapevine leaf. To detect the extraction product, we performed a PCR We compared it to a traditional kit-based extraction, and to our synthetic endogeneous control sequence (EC sequence). The kit we used is DNeasy Plant Pro Kit by Qiagen.
DNA Extraction:
We performed a Nanodrop UV absorption spectra with the DNA extracted with the microneedle patches, an endegeneous control, and elution buffer applied on an unused microneedle patch as a control.
The green Line corresponds to the EC;
The red Line corresponds to the Microneedle extraction;
The blue Line corresponds to the elution buffer applied on an unused microneedle patch (no DNA).