Team:EPFL/Parts

ViTEST

• Overview
  • Description
  • Design
  • Results
• Project
  • Project
  • DNA Extraction
  • DNA Amplification
  • Detection
  • OnePot Pure
  • Hardware
  • Demonstrate
  • Protocols
  • Notebook
• Parts
  • Parts Overview
  • Part Collection
  • Improve/Contribution
• Human Practices
  • Integrated Human Practices
  • Education and Public Engagement
• Team
  • Team Members
  • Collaborations
  • Attributions
• Awards
• Safety
• Judging Form

Parts

Our parts collection consists of thirty two basic parts and fifty five composite parts. All parts were generated during our project. Some of them are related to the PURE cell-free system, while others are application of Toehold switches.

Basic Parts

The table below contains all the basic parts. The information provided on the table are: part name that links to the part page in the registry, part type, short description, and length.

Name	Type	Description	Length
BBa_K2916000	Coding	ArgRS protein equipped with a 10x HIS affinity tag	1800
BBa_K2916001	Coding	GlnRS protein equipped with a 6x HIS affinity tag	1706
BBa_K2916002	Coding	GluRS protein equipped with a 6x HIS affinity tag	1457
BBa_K2916003	Coding	GluRS protein equipped with a 6x HIS affinity tag	3032
BBa_K2916004	Coding	IleRS protein equipped with a 6x HIS affinity tag	2894
BBa_K2916005	Coding	LeuRS protein equipped with a 6x HIS affinity tag	2624
BBa_K2916006	Coding	LysRS protein equipped with a 6x HIS affinity tag	1559
BBa_K2916007	Coding	PheRS protein equipped with a 6x HIS affinity tag	3483
BBa_K2916008	Coding	EF-G protein equipped with a 6x HIS affinity tag	2148
BBa_K2916009	Coding	EF-Tu protein equipped with a 6x HIS affinity tag	1218
BBa_K2916010	Coding	CK protein equipped with a 6x HIS affinity tag	1185
BBa_K2916011	Coding	MK protein equipped with a 6x HIS affinity tag	627
BBa_K2916012	Coding	NDK protein eqquiped with a 6x HIS affinity tag	501
BBa_K2916013	Coding	PPiase protein equipped with a 6x HIS affinity tag	905
BBa_K2916050	Regulatory	Toehold for Grapevine Endogenous Control 1.1	125
BBa_K2916051	Regulatory	Toehold for Grapevine Endogenous Control 2.1	122
BBa_K2916052	Regulatory	Toehold for Grapevine Endogenous Control 2.2	122
BBa_K2916053	Regulatory	Toehold for Grapevine Endogenous Control 2.3	122
BBa_K2916054	Regulatory	Toehold for Grapevine Endogenous Control 2.4	122
BBa_K2916055	Regulatory	Toehold for Bois Noir 1.1	125
BBa_K2916056	Regulatory	Toehold for Bois Noir 1.2	125
BBa_K2916057	Regulatory	Toehold for Bois Noir 1.3	125
BBa_K2916058	Regulatory	Toehold for Bois Noir 1.4	125
BBa_K2916059	Regulatory	Toehold for Bois Noir 2.1	122
BBa_K2916060	Regulatory	Toehold for Bois Noir 2.2	122
BBa_K2916061	Regulatory	Toehold for Bois Noir 2.3	122
BBa_K2916062	Regulatory	Toehold for Bois Noir 2.4	122
BBa_K2916063	Regulatory	Toehold for Flavescence Doree 1.1	125
BBa_K2916064	Regulatory	Toehold for Flavescence Doree 2.1	122
BBa_K2916065	Regulatory	Toehold for Flavescence Doree 2.2	122
BBa_K2916066	Regulatory	Toehold for Flavescence Doree 2.3	122
BBa_K2916067	Regulatory	Toehold for Flavescence Doree 2.4	122

Composite Parts

The table below contains all the composite parts. The information provided on the table are: part name that links to the part page in the registry, part type, short description, and length.

Name	Type	Description	Length
BBa_K2916014	Composite	Expression of AlaRS in E.coli	2888
BBa_K2916015	Composite	Expression of ArgRS in E.coli	1950
BBa_K2916016	Composite	Expression of AsnRS in E.coli	1658
BBa_K2916017	Composite	Expression of AspRS in E.coli	1968
BBa_K2916018	Composite	Expression of CysRS in E.coli	1581
BBa_K2916019	Composite	Expression of GlnRS in E.coli	1856
BBa_K2916020	Composite	Expression of GluRS in E.coli	1607
BBa_K2916021	Composite	Expression of GlyRS in E.coli	3182
BBa_K2916022	Composite	Expression of HisRS in E.coli	1470
BBa_K2916023	Composite	Expression of IleRS in E.coli	3044
BBa_K2916024	Composite	Expression of LeuRS in E.coli	2774
BBa_K2916025	Composite	Expression of LysRS in E.coli	1709
BBa_K2916026	Composite	Expression of MetRS in E.coli	2229
BBa_K2916027	Composite	Expression of PheRS in E.coli	3695
BBa_K2916028	Composite	Expression of ProRS in E.coli	1914
BBa_K2916029	Composite	Expression of SerRS in E.coli	1488
BBa_K2916030	Composite	Expression of ThrRS in E.coli	2186
BBa_K2916031	Composite	Expression of ThrRS in E.coli	1200
BBa_K2916032	Composite	Expression of ThrRS in E.coli	1470
BBa_K2916033	Composite	Expression of ValRS in E.coli	3051
BBa_K2916034	Composite	Expression of IF1 in E.colI	482
BBa_K2916035	Composite	Expression of IF2 in E.colI	2930
BBa_K2916036	Composite	Expression of IF3 in E.colI	800
BBa_K2916037	Composite	Expression of EF-G in E.coli	2360
BBa_K2916038	Composite	Expression of EF-Tu in E.coli	2360
BBa_K2916039	Composite	Expression of EF-Ts in E.coli	1109
BBa_K2916040	Composite	Expression of RF1 in E.coli	1340
BBa_K2916041	Composite	Expression of RF2 in E.coli	1293
BBa_K2916042	Composite	Expression of RF3 in E.coli	1847
BBa_K2916043	Composite	Expression of RFF in E.coli	815
BBa_K2916044	Composite	Expression of MTF in E.coli	1143
BBa_K2916045	Composite	Expression of CK in E.coli	1403
BBa_K2916046	Composite	Expression of MK in E.coli	777
BBa_K2916047	Composite	Expression of NDK in E.coli	713
BBa_K2916048	Composite	Expression of PPiase in E.coli	1055
BBa_K2916049	Composite	Expression of T7 RNA in E.coli	2912
BBa_K2916068	Composite	Toehold switch for Grapevine Endogenous control 1.1 with superfolding GFP	988
BBa_K2916069	Composite	Toehold switch for Bois Noir 1.1 with superfolding GFP	988
BBa_K2916070	Composite	Toehold switch for Bois Noir 1.2 with superfolding GFP	988
BBa_K2916071	Composite	Toehold switch for Bois Noir 1.3 with superfolding GFP	988
BBa_K2916072	Composite	Toehold switch for Bois Noir 14 with superfolding GFP	988
BBa_K2916073	Composite	Toehold switch for Bois Noir 2.1 with superfolding GFP	985
BBa_K2916074	Composite	Toehold switch for Bois Noir 2.2 with superfolding GFP	985
BBa_K2916075	Composite	Toehold switch for Bois Noir 2.3 with superfolding GFP	985
BBa_K2916076	Composite	Toehold switch for Bois Noir 2.4 with superfolding GFP	985
BBa_K2916077	Composite	Toehold switch for Flavescence Doree 1.1 with superfolding GFP	988
BBa_K2916078	Composite	Toehold switch for Grapevine Endogenous control 2.1 with CDO	1052
BBa_K2916079	Composite	Toehold switch for Grapevine Endogenous control 2.2 with CDO	1052
BBa_K2916080	Composite	Toehold switch for Grapevine Endogenous control 2.3 with CDO	1052
BBa_K2916081	Composite	Toehold switch for Grapevine Endogenous control 2.4 with CDO	1052
BBa_K2916082	Composite	Toehold switch for Bois Noir 2.1 with CDO	1052
BBa_K2916083	Composite	Toehold switch for Flavescence Doree 2.1 with CDO	1055
BBa_K2916084	Composite	Toehold switch for Flavescence Doree 2.2 with CDO	1052
BBa_K2916085	Composite	Toehold switch for Flavescence Doree 2.3 with CDO	1052
BBa_K2916086	Composite	Toehold switch for Flavescence Doree 2.4 with CDO	1052

Best Basic Part

EF-Tu protein: BBa_K2916009

We have chosen to nominate EF-Tu as our best basic part. It is an indispensable component in the PURE cell-free translation-transcription system. The main function of the EF-Tu (elongation factor-thermo unstable) is to catalyze the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosomes. It plays a crucial role in the OnePot PURE cell-free system as it accounts for 47% of the total protein concentration in the protein solution. The sequence, apart from the gene encoding, also includes a 6x HIS affinity tag, to allow the purification of the expressed protein.

Best Composite Part

Toehold switch for Bois Noir 2.1 with superfolding GFP : BBa_K2916073

The ultimate goal of our project is to detect specific sequences of the two diseases in question, so for Best Composite Part we nominate Toehold switch for Bois Noir 2.1 with superfolding GFP. This part, although it is quite particular to our project may act as a proof of concept for future iGEM teams that also want to implement toehold switches in their project. More quantitative and qualitative information about this part can be found in its registry page

References