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Team:EPFL/Parts


Parts

Our parts collection consists of thirty two basic parts and fifty five composite parts. All parts were generated during our project. Some of them are related to the PURE cell-free system, while others are application of Toehold switches.

The table below contains all the composite parts. The information provided on the table are: part name that links to the part page in the registry, part type, short description, and length.

Name Type Description Length
BBa_K2916014 Composite Expression of AlaRS in E.coli 2888
BBa_K2916015 Composite Expression of ArgRS in E.coli 1950
BBa_K2916016 Composite Expression of AsnRS in E.coli 1658
BBa_K2916017 Composite Expression of AspRS in E.coli 1968
BBa_K2916018 Composite Expression of CysRS in E.coli 1581
BBa_K2916019 Composite Expression of GlnRS in E.coli 1856
BBa_K2916020 Composite Expression of GluRS in E.coli 1607
BBa_K2916021 Composite Expression of GlyRS in E.coli 3182
BBa_K2916022 Composite Expression of HisRS in E.coli 1470
BBa_K2916023 Composite Expression of IleRS in E.coli 3044
BBa_K2916024 Composite Expression of LeuRS in E.coli 2774
BBa_K2916025 Composite Expression of LysRS in E.coli 1709
BBa_K2916026 Composite Expression of MetRS in E.coli 2229
BBa_K2916027 Composite Expression of PheRS in E.coli 3695
BBa_K2916028 Composite Expression of ProRS in E.coli 1914
BBa_K2916029 Composite Expression of SerRS in E.coli 1488
BBa_K2916030 Composite Expression of ThrRS in E.coli 2186
BBa_K2916031 Composite Expression of ThrRS in E.coli 1200
BBa_K2916032 Composite Expression of ThrRS in E.coli 1470
BBa_K2916033 Composite Expression of ValRS in E.coli 3051
BBa_K2916034 Composite Expression of IF1 in E.colI 482
BBa_K2916035 Composite Expression of IF2 in E.colI 2930
BBa_K2916036 Composite Expression of IF3 in E.colI 800
BBa_K2916037 Composite Expression of EF-G in E.coli 2360
BBa_K2916038 Composite Expression of EF-Tu in E.coli 2360
BBa_K2916039 Composite Expression of EF-Ts in E.coli 1109
BBa_K2916040 Composite Expression of RF1 in E.coli 1340
BBa_K2916041 Composite Expression of RF2 in E.coli 1293
BBa_K2916042 Composite Expression of RF3 in E.coli 1847
BBa_K2916043 Composite Expression of RFF in E.coli 815
BBa_K2916044 Composite Expression of MTF in E.coli 1143
BBa_K2916045 Composite Expression of CK in E.coli 1403
BBa_K2916046 Composite Expression of MK in E.coli 777
BBa_K2916047 Composite Expression of NDK in E.coli 713
BBa_K2916048 Composite Expression of PPiase in E.coli 1055
BBa_K2916049 Composite Expression of T7 RNA in E.coli 2912
BBa_K2916068 Composite Toehold switch for Grapevine Endogenous control 1.1 with superfolding GFP 988
BBa_K2916069 Composite Toehold switch for Bois Noir 1.1 with superfolding GFP 988
BBa_K2916070 Composite Toehold switch for Bois Noir 1.2 with superfolding GFP 988
BBa_K2916071 Composite Toehold switch for Bois Noir 1.3 with superfolding GFP 988
BBa_K2916072 Composite Toehold switch for Bois Noir 14 with superfolding GFP 988
BBa_K2916073 Composite Toehold switch for Bois Noir 2.1 with superfolding GFP 985
BBa_K2916074 Composite Toehold switch for Bois Noir 2.2 with superfolding GFP 985
BBa_K2916075 Composite Toehold switch for Bois Noir 2.3 with superfolding GFP 985
BBa_K2916076 Composite Toehold switch for Bois Noir 2.4 with superfolding GFP 985
BBa_K2916077 Composite Toehold switch for Flavescence Doree 1.1 with superfolding GFP 988
BBa_K2916078 Composite Toehold switch for Grapevine Endogenous control 2.1 with CDO 1052
BBa_K2916079 Composite Toehold switch for Grapevine Endogenous control 2.2 with CDO 1052
BBa_K2916080 Composite Toehold switch for Grapevine Endogenous control 2.3 with CDO 1052
BBa_K2916081 Composite Toehold switch for Grapevine Endogenous control 2.4 with CDO 1052
BBa_K2916082 Composite Toehold switch for Bois Noir 2.1 with CDO 1052
BBa_K2916083 Composite Toehold switch for Flavescence Doree 2.1 with CDO 1055
BBa_K2916084 Composite Toehold switch for Flavescence Doree 2.2 with CDO 1052
BBa_K2916085 Composite Toehold switch for Flavescence Doree 2.3 with CDO 1052
BBa_K2916086 Composite Toehold switch for Flavescence Doree 2.4 with CDO 1052

Best Basic Part

EF-Tu protein: BBa_K2916009

We have chosen to nominate EF-Tu as our best basic part. It is an indispensable component in the PURE cell-free translation-transcription system. The main function of the EF-Tu (elongation factor-thermo unstable) is to catalyze the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosomes. It plays a crucial role in the OnePot PURE cell-free system as it accounts for 47% of the total protein concentration in the protein solution. The sequence, apart from the gene encoding, also includes a 6x HIS affinity tag, to allow the purification of the expressed protein.

Best Composite Part

Toehold switch for Bois Noir 2.1 with superfolding GFP : BBa_K2916073

The ultimate goal of our project is to detect specific sequences of the two diseases in question, so for Best Composite Part we nominate Toehold switch for Bois Noir 2.1 with superfolding GFP. This part, although it is quite particular to our project may act as a proof of concept for future iGEM teams that also want to implement toehold switches in their project. More quantitative and qualitative information about this part can be found in its registry page

References