During our Experiments we used abreviations to simplify the results annotation. MN = Microneedle FD = Flavescence Dorée BN = Bois Noir EC = Endogeneous Control (DNA from grapevines)
To test our method of extraction, we used non-infected grapevine leaf.
To detect the product of the extraction, we performed an amplification by PCR. We compared the extraction by microneedles
to a traditional kit-based extraction. The control is synthetic endogeneous control DNA sequence (EC sequence).
We used the Nanodrop to analyze the DNA extracted with a microneedle patch (red). The positive control (green) is EC synthetic DNA sequence and the negative control (blue) is the elution buffer applied on an unused microneedle patch. As expected, the negative control absorption at 260nm is close to zero, no DNA is present in the microneedle patch. In contrast, the positive control has the typical DNA Nanodrop readout. The graph of DNA extracted by microneedle patch shows a peak of absorption at 260nm, we can also see that that values absorption values at 230nm and 280nm are lower that the value at 260nm. Overall, the graph is similar to the positive control but with lower values. This indicates that DNA was extracted by the microneedle patch.