DNA Extraction
To test our method of extraction, we decided to try it on a non-infected grapevine leaf. To detect the extraction product, we performed a PCR We compared it to a traditional kit-based extraction, and to our synthetic endogeneous control sequence (EC sequence). The kit we used is DNeasy Plant Pro Kit by Qiagen.
DNA Extraction:
Here we performed a Nanodrop UV absorption spectra with the DNA extracted with the micro needle patches and an endogeneous control :
The red Line corresponds to the endogeneous control (EC);
The black Line corresponds to the Micro Needle extraction.
2nd graph. Green Line corresponds to gBlock;
Red Line corresponds to the Micro Needle extraction;
Blue Line corresponds to control (no DNA).