Team:TUDelft/Test
Engineering organism specific parts and utilizing existing parts across different species is extremely laborious and expensive
which restricts the scope of synthetic biology to a small subset of the bacterial cosmos.
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In our project, we developed the Sci-Phi 29 platform that aims to expand the repertoire of bacterial species and broaden the range of substrates and environmental conditions which is currently applied in synthetic biology.
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How does Sci-Phi 29 work?
Sci-Phi 29
is a standardized and user-friendly platform which allows expressing of your gene of interest in a controllable manner across different bacterial species and independently of the host.
To express genetic tools across multiple bacterial species, we were inspired by the replication machinery of the phi29 bacteriophage. Its unique protein-primed based replication greatly simplifies the design of replication systems. This system is able to replicate a linear piece of DNA by using only four proteins.
To tackle the issue of variation in expression across species, we took our platform to the next level by integrating the concept of controllability, which is based on a systems engineering approach.
We included the following variables in our model and experiments:
To envision a future where our Sci-Phi 29 platform can be used to tackle a real world problem, we created a hypothetical use-case scenario. We theoretically applied Sci-Phi 29 to engineer P. putida to be able to convert microplastics from waste water streams.
Explore the microbial world with us!
We as a team are fascinated by this microbial diversity and wanted to share our fascination with the rest of the world. That is why our goal this year was to introduce the general public, from children to your neighbor to your teacher, to the hidden world of microbes. We organized multiple events because we wanted to make sure that everyone has access to the invisible microbial world.
Find out more about other aspects of our project
