Team:TUDelft/ResultsTest

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Project

Description Design Results Demonstrate

Wetlab

Experiment Protocols Safety Notebook

Modeling

Model

Reaction Kinetics

Copy Number independence Transcription Variation Translation Variation

Codon Harmonization

Parts

Parts Overview

Human Practices

Human Practices Education & Engagement

Team

Team Members Collaborations

Acknowledgements

Attributions Sponsors

Judging Form Medal Requirements

 

• Overview
• Parts Construction
• Parts Characterization
• Orthogonality
• Replication
• Toxicity Assay
• Controllability
• Plasmid Copy Number
• Transcriptional Variations
• Translational Variations
• Harmonization (Qualitative)
• Future Plans

Overview

Our Motivation

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Sci-Phi 29: Enabling orthogonal replication and predictable expression to expand the repertoire of engineerable bacteria

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Orthogonalibity

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Controllabillity

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Standardization of Sci-Phi 29

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Impact of Sci-Phi 29

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References

• Sun, D. (2018). Pull in and Push Out: Mechanisms of Horizontal Gene Transfer in Bacteria. Frontiers in Microbiology, 9, 2154. https://doi.org/10.3389/fmicb.2018.02154
• Weber, E., Engler, C., Gruetzner, R., Werner, S., & Marillonnet, S. (2011). A modular cloning system for standardized assembly of multigene constructs. PloS One, 6(2), e16765. http://doi.org/10.1371/journal.pone.0016765

Special thanks to our sponsors