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Best Basic Part﹀
♥ Name Description Designer ♥ BBa_K2918000 blablablablablablablablablablablabla TU Delft 2019
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Best Composite Part﹀
♥ Name Description Designer ♥ BBa_K2918000 blablablablablablablablablablablabla TU Delft 2019 -
Best Part Collection﹀
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Engineering non-model organisms requires discovery of organism-specific parts or re-tuning of parts characterized in model organisms. Our part collection provides modular cloning (MoClo) compatible parts that can be used for predictable expression of genes across different bacterial species.
To overcome the need to identify parts for different bacterial hosts, our collection provides parts of the phi29 replication machinery to establish orthogonal replication. Furthermore, the collection consists of regulatory elements (promoters, RBSs, terminators) that work in a plethora of bacterial species.
To insulate the behavior of genetic circuits from variations associated with change in bacterial context, our collection also offers parts essential to append an incoherent feed forward loop (iFFL) to any genetic circuit. iFFL ensures that the gene of interest expression is impervious to changes in copy number, transcriptional and translational rates.
Our collection acts as a platform to future iGEM teams for effortless engineering of their bacteria of choice.
Basic Parts
Name Type Description Designer Length BBa_K2918005 Regulatory 0.1 T7 promoter TUDelft2019 23 BBa_K2918006 Regulatory 0.5 T7 promoter TUDelft2019 23 BBa_K2918007 Regulatory Wild type T7 promoter TUDelft2019 23 BBa_K2918009 Regulatory 0.5 T7 promoter variant with TALEsp1 binding site TUDelft 2019 41 BBa_K2918035 RBS BBa_B0032 RBS and RiboJ TUDelft 2019 94 BBa_K2918013 RBS BBa_B0032 RBS and SarJ TUDelft 2019 98 BBa_K2918034 Regulatory Broad host range promoter TUDelft 2019 56 BBa_K2918011 Regulatory Broad host range promoter with TALEsp1 binding site TUDelft 2019 56 BBa_K2918037 Coding Cross-species harmonized GFP TUDelft 2019 678 BBa_K2918061 Other RBS (B0032) adapted for modular cloning with Ribozymes TUDelft 2019 25 BBa_K2918038 RBS RiboJ and universal binding site TUDelft 2019 85 BBa_K2918012 Other Ribozyme J (Ribo J) TUDelft 2019 75 BBa_K2918036 RBS SarJ and universal ribosome binding site TUDelft 2019 89 BBa_K2918010 Regulatory T7 promoter with TALEsp1 binding site TUDelft 2019 41 BBa_K2918016 Terminator T7 terminator variant TUDelft 2019 48 BBa_K2918008 Coding Transcription Activator like Effector protein (TALEsp1) TUDelft2019 2640 BBa_K2918014 RBS Universal RBS TUDelft2019 10 BBa_K2918062 RBS Universal RBS site adapted for modular cloning with ribozymes TUDelft 2019 16 BBa_K2918015 Terminator Wild type T7 terminator TUDelft2019 48 BBa_K2918000 Coding Φ29 DNA polymerase (DNAP/p2) TUDelft2019 1719 BBa_K2918003 Coding Φ29 Double Strand Binding Protein (DSB/p6) TUDelft2019 315 BBa_K2918002 Coding Φ29 Single Stranded Binding Protein (SSB/p5) TUDelft2019 375 BBa_K2918001 Coding Φ29 Terminal Protein (TP/p3) TUDelft2019 801
Composite Parts
Name Type Description Designer Length BBa_K2918017 Composite 0.1 T7 promoter - Universal RBS - Φ29 9 TP - WT T7 terminator TUDelft2019 890 BBa_K2918018 Composite 0.5 T7 promoter - Universal RBS - Φ29 TP - T7 terminator TUDelft2019 904 BBa_K2918019 Composite WT T7 promoter - Universal RBS - Φ29 TP - T7 terminator TUDelft2019 904 BBa_K2918020 Composite 0.1 T7 promoter - Universal RBS - Φ29 DNAP - T7 terminator TUDelft2019 1822 BBa_K2918021 Composite 0.5 T7 promoter - Universal RBS - Φ29 DNAP - T7 terminator TUDelft2019 1822 BBa_K2918022 Composite WT T7 promoter - Universal RBS - Φ29 DNAP - WT T7 terminator TUDelft2019 1899 BBa_K2918023 Composite 0.1 T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator TUDelft2019 519 BBa_K2918024 Composite 0.5 T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator TUDelft2019 519 BBa_K2918025 Composite WT T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator TUDelft2019 519 BBa_K2918026 Composite 0.1 T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator TUDelft2019 579 BBa_K2918027 Composite 0.5 T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator TUDelft2019 579 BBa_K2918028 Composite WT T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator TUDelft2019 579 BBa_K2918030 Composite WT T7 promoter - Universal RBS - GFP - T7 terminator TUDelft2019 811 BBa_K2918031 Composite 0.1 T7 promoter - Universal RBS - GFP - T7 terminator TUDelft2019 811 BBa_K2918032 Composite 0.5 T7 promoter - Universal RBS - GFP - T7 terminator TUDelft2019 811
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