Team:TUDelft/DennisModel
Overview - The Model
I In our system we exploit a commonly applied control system known as an Incoherent Feed Forward Loop (iFFL), in which an activator regulates both a gene and a repressor of the gene ... . This control system is established through the expression of a Transcription activator-like effector (TALE) protein. TALE proteins recognize DNA by a simple DNA-binding mechanism which can be altered to recognize any sequence you want … . In our system the TALE protein binds to the promoter of a gene of interest and thus represses the expression of it. .... has previously described this system and showed how it results in independence of copy number for a gene of interest. Our further analyzation of this system has revealed the system to be independent of many other variables. We exploit this robustness of the system to show how it can yield predictable expression when transferring your genetic circuit between prokaryotes.