Team:TUDelft/DennisModel
Overview
The main goal of our project revolves around predictability. Without predictability, we cannot reliably design and implement synthetic biology solutions. However, when transferring genetic circuits between different organisms, many variables change. This dramatically increases the uncertainty of the outcome. With our modeling, we aim to demonstrate ways to make a genetic circuit independent of some of these variables, thereby decreasing this uncertainty. We couple our model with a software tool that determines a coding sequence for each gene involved in the circuit in such a way that they have similar codon usage bias across species of interest.
TALE - Incoherent Feed Forward Loop to control gene expression
In our system we make use of a commonly applied control system called an Incoherent Feed Forward Loop developed by ... . This control system is established through the expression of a TALE protein which binds to the promoter of a gene of interest and thus repressing the expression.