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<center style="font-size:large;"><b>Find more about how we applied this unique system in our project!</b></center> | <center style="font-size:large;"><b>Find more about how we applied this unique system in our project!</b></center> | ||
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− | <center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Design" class="buttontu "><b>Read More</b></a></center> | + | <center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Design#orthorep" class="buttontu "><b>Read More</b></a></center> |
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<br><br> <center style="font-size:large;"><b>Do you want to learn more about the details?</b></center> | <br><br> <center style="font-size:large;"><b>Do you want to learn more about the details?</b></center> | ||
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− | <center><a href="https://2019.igem.org/Team:TUDelft/Design" class="buttontu"><b>Click here</b></a></center> | + | <center><a href="https://2019.igem.org/Team:TUDelft/Design#control" class="buttontu"><b>Click here</b></a></center> |
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Revision as of 04:36, 14 December 2019
Engineering organism-specific parts and utilizing existing parts across different species is extremely laborious and expensive
which restricts the scope of synthetic biology to a small subset of the bacterial cosmos.
![Parts road](https://static.igem.org/mediawiki/2019/8/84/T--TUDelft--steps_parts.png)
.
![Sci-Phi 29](https://static.igem.org/mediawiki/2019/e/e7/T--TUDELFT--logonavbar-white.png)
.
We developed Sci-Phi 29, a platform that expands the scope of synthetic biology by providing essential tools required to engineer a repertoire of bacterial species.
Read More
How does Sci-Phi 29 work?
![Different Bacteria](https://static.igem.org/mediawiki/2019/1/1c/T--TUDelft--Bacteria_home.png)
![Arrow](https://static.igem.org/mediawiki/2019/d/de/T--TUDelft--ArrowPattern.png)
![Different Bacteria](https://static.igem.org/mediawiki/2019/d/d3/T--TUDelft--Sci-PhiKit.png)
Sci-Phi 29
A user-friendly platform that allows engineering your bacteria of choice through:
![Arrow split](https://static.igem.org/mediawiki/2019/9/9e/T--TUDelft--Arrowpatternsplit.png)
![Orthogonality](https://static.igem.org/mediawiki/2019/f/f0/T--TUDelft--logorepli.png)
To express genetic tools across multiple bacterial species, we were inspired by the replication machinery of the phi29 bacteriophage. Its unique protein-primed based replication greatly simplifies the design of replication systems. This system is able to replicate a linear piece of DNA by using only four proteins.
![Orthogonal Replication System](https://static.igem.org/mediawiki/2019/2/2d/T--TUDelft--orthoreplihome.png)
![Controllability](https://static.igem.org/mediawiki/2019/5/56/T--TUDelft--logocontrol.png)
To tackle the issue of variation in expression across bacterial species, we applied control systems thinking to achieve controllability.
We included the following variables in our model and experiments:
![Sci-Phi 29 applications](https://static.igem.org/mediawiki/2019/2/21/T--TUDelft--sci-phitogether.png)
To envision a future where our Sci-Phi 29 platform can be used to tackle a real world problem, we created a hypothetical use-case scenario. We theoretically applied Sci-Phi 29 to engineer P. putida to be able to convert microplastics from waste water streams.
Explore the microbial world with us!
We as a team are fascinated by this microbial diversity and wanted to share our fascination with the rest of the world. That is why our goal this year was to introduce the general public, from children to your neighbor to your teacher, to the hidden world of microbes. We organized multiple events because we wanted to make sure that everyone has access to the invisible microbial world.
Click here to meet our team!
![Team Picture](https://static.igem.org/mediawiki/2019/2/23/T--TUDelft--Team2.png)