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Revision as of 15:55, 18 November 2019


Achievements

Grand Prize

Photo credit : iGEM Foundation - Justin Knight




Grand Prize Winner


Our team was announced as the Grand Prize Winner in the Overgrad category !

Awards





Best Environment Project

Best Integrated Human Practices



Nominations




Best Part Collection

Best Presentation

Best Wiki

Medal




Medal Requirements


Bronze Medal

Silver Medal

Gold Medal




  • Registration and Giant Jamboree Attendance

    • Our team participated in the 2019 Giant Jamboree!

  • Competition Deliverables

    • We gave our presentation and showed our poster at the Jamboree

  • Attributions

    • We would like to thank all the people who helped us with our project.

  • Project inspiration and Description

    • Flavescence Dorée is a big problem in vineyards in Europe, the biggest issue at this point is the detection takes a long time.

  • Characterization/ contribution

    • Our project is mainly based on homemade cell-free system, OnePot PURE. We wanted to prove that the gene XylE (part Bba_I746909) could be successfully expressed in OnePot PURE. The expression of this gene creates the enzyme catechol 2,3-dioxygenase(CDO) which creates a yellow color by reacting with the substrate catechol .




  • Validated part

    • We created new parts, that are necessary to produce OnePot PURE

  • Collaboration

    • We collaborated with different teams such as Thessaly, UZurich, ETH Zurich and UniGE. We also participated in a few meetups across Europe.

  • Human practices

    • As an iGEM team we had the opportunity to teach high schoolers about synthetic biology. We also presented our project to the public during EPFL’s open doors and created a tutorial on how to make OnePot PURE.




  • Integrated Human practices

    • Integrated Human practices shaped our project. We worked with all the organizations involved in the detection of Flavescence Dorée.

  • Improve a Previous part/project


    • We improved the part sfGFP BBa_I746916 by adding toehold switches to it. This protein can be expressed only if the specific trigger mRNA is also present in the cell free system.

  • Demonstration of your work

    • We demonstrate how we managed to link each parts of the project, creating a proof or concept of our work.






© iGEM EPFL 2019