• 
  Best Environment Project

• 
  Best Integrated Human Practices

Bronze Medal

Silver Medal

Gold Medal

• Registration and Giant Jamboree Attendance

Our team participated in the 2019 Giant Jamboree!

• Competition Deliverables

We gave our presentation and showed our poster at the Jamboree

• Attributions

We would like to thank all the people who helped us with our project.

• Project inspiration and Description

Flavescence Dorée is a big problem in vineyards in Europe, the biggest issue at this point is the detection takes a long time.

• Characterization/ contribution

Our project is mainly based on homemade cell-free system, OnePot PURE. We wanted to prove that the gene XylE (part Bba_I746909) could be successfully expressed in OnePot PURE. The expression of this gene creates the enzyme catechol 2,3-dioxygenase(CDO) which creates a yellow color by reacting with the substrate catechol .

• Validated part

We created new parts, that are necessary to produce OnePot PURE

• Collaboration

We collaborated with different teams such as Thessaly, UZurich, ETH Zurich and UniGE. We also participated in a few meetups across Europe.

• Human practices

As an iGEM team we had the opportunity to teach high schoolers about synthetic biology. We also presented our project to the public during EPFL’s open doors and created a tutorial on how to make OnePot PURE.

• Integrated Human practices

Integrated Human practices shaped our project. We worked with all the organizations involved in the detection of Flavescence Dorée.

• Improve a Previous part/project


We improved the part sfGFP BBa_I746916 by adding toehold switches to it. This protein can be expressed only if the specific trigger mRNA is also present in the cell free system.

• Demonstration of your work

We demonstrate how we managed to link each parts of the project, creating a proof or concept of our work.