Weronika77 (Talk | contribs) |
Weronika77 (Talk | contribs) |
||
Line 926: | Line 926: | ||
<td>Level M constructs (L1D + L1E, L1G + L1B) were both cloned in low and medium copy backbones. The constructs were then co-transformed with <a href="https://www.addgene.org/71428/">MK1283</a> which contains a plasmid harbouring a T7 RNAP mixed forward loop. Fluorescence was detected by FACS. | <td>Level M constructs (L1D + L1E, L1G + L1B) were both cloned in low and medium copy backbones. The constructs were then co-transformed with <a href="https://www.addgene.org/71428/">MK1283</a> which contains a plasmid harbouring a T7 RNAP mixed forward loop. Fluorescence was detected by FACS. | ||
<ul> | <ul> | ||
− | <li>L1_A: | + | <li>L1_A: <a href="http://parts.igem.org/Part:BBa_K2918011">PromoterBHRsp1</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_J97005">Juniper GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li> |
− | + | <li>L1_B: <a href="http://parts.igem.org/Part:BBa_K2918010">T7sp1 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918037">Harmonized GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li> | |
− | + | <li>L1_C: <a href="http://parts.igem.org/Part:BBa_K2918000">Pbhr</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a> -<a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li> | |
− | + | <li>L1_D: <a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a> - <a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li> | |
− | + | <li>L1_E: <a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918037">Harmonized GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li> | |
− | + | <li>L1_F: <a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a> - <a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li> | |
− | + | <li>L1_G: <a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a>- <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li> | |
− | + | <li>L1_H: <a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918037">Harmonized GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li> | |
</ul> | </ul> | ||
</td></tr> | </td></tr> |
Revision as of 22:22, 21 October 2019
![Design](https://static.igem.org/mediawiki/2019/e/e4/T--TUDelft--Notebook_logo.png)
Orthogonal Replication
Cloning | Testing of Plasmid | |||
---|---|---|---|---|
April |
|
|
||
May | ||||
June | ||||
July |
|
|||
August |
|
|
||
September |
|
|
||
October |
|
Controllable Expression
Cloning | Testing of Plasmid | |||
---|---|---|---|---|
April | ||||
May | ||||
June |
|
|||
July | ||||
August |
|
|
||
September |
|
|
|
|
October |