Difference between revisions of "Team:TUDelft/Notebook"

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                       <a href="https://2019.igem.org/Team:TUDelft/Experiments"><i>E. coli</i> Heat Shock Transformation</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments">KOD Extreme PCR</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments">Colony Polymerase PCR (GoTaq)</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments"><i>E. coli</i> Glycerol Stock</a>,
 
                       <a href="https://2019.igem.org/Team:TUDelft/Experiments"><i>E. coli</i> Heat Shock Transformation</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments">KOD Extreme PCR</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments">Colony Polymerase PCR (GoTaq)</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments"><i>E. coli</i> Glycerol Stock</a>,
 
                       <a href="https://2019.igem.org/Team:TUDelft/Experiments">DNA Gel Electrophoresis</a>,
 
                       <a href="https://2019.igem.org/Team:TUDelft/Experiments">DNA Gel Electrophoresis</a>,
−
                       <a href="https://2019.igem.org/Team:TUDelft/Experiments">Sequencing</a>
+
                       <a href="https://2019.igem.org/Team:TUDelft/Experiments">Sequencing</a>,
 +
<a href="https://2019.igem.org/Team:TUDelft/Experiments">IPTG stock</a>
 
                     </td>
 
                     </td>
 
                   </tr>
 
                   </tr>
Line 387: Line 388:
 
                         Subsequently, the level 0's were used to assembly the following level 1 constructs:
 
                         Subsequently, the level 0's were used to assembly the following level 1 constructs:
 
                       <ul>
 
                       <ul>
−
                         <li><a href="https://2019.igem.org/Team:TUDelft/Experiments">IPTG stock</a> 0.1 T7 promoter - Uni RBS - DNAP - WT T7 terminator</li>
+
                         <li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918034">DNAP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918034">DNAP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918034">DNAP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918001">TP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918001">TP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918001">TP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918002">p5</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918002">p5</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918002">p5</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918003">p6</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918003">p6</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918003">p6</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - GFP - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - GFP - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - GFP - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
 
 +
 
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918020">OriL-GFP-Kan-OriR</a></li>
 +
                            </ul>
 +
0.1 T7 promoter - Uni RBS - DNAP - WT T7 terminator</li>
 
                         <li>0.5 T7 promoter - Uni RBS - DNAP - WT T7 terminator</li>
 
                         <li>0.5 T7 promoter - Uni RBS - DNAP - WT T7 terminator</li>
 
                         <li>WT T7 promoter - Uni RBS - DNAP - WT T7 terminator</li>
 
                         <li>WT T7 promoter - Uni RBS - DNAP - WT T7 terminator</li>
Line 786: Line 807:
 
                       <br>
 
                       <br>
 
                       <ul>
 
                       <ul>
−
                         <li>WT T7 promoter - Uni RBS - Harmonized GFP - WT T7 terminator</li>
+
                         <li><a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918037">Harmonized GFP</a> -<a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>L1_A: Promoter<sub>BHR</sub>sp1-RiboJ - Uni RBS - Juniper GFP - WT T7 terminator</li>
+
                                <li>L1_A: <a href="http://parts.igem.org/Part:BBa_K2918011">PromoterBHRsp1</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_J97005">Juniper GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>L1_B: T7_sp1 promoter - RiboJ - Uni RBS - Harmonized GFP - WT T7 terminator</li>
+
                                <li>L1_B: <a href="http://parts.igem.org/Part:BBa_K2918010">T7sp1 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918037">Harmonized GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>L1_C: Promoter<sub>BHR</sub> - Sarj-Uni RBS - TALEsp1 - WT T7 terminator variant</li>
+
                                <li>L1_C: <a href="http://parts.igem.org/Part:BBa_K2918000">Pbhr</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a> -<a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li>
−
                        <li>L1_D: T7 promoter - SarJ-Uni RBS - TALEsp1 - WT T7 terminator variant </li>
+
                                <li>L1_D: <a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a> - <a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li>
−
                        <li>L1_E: T7 promoter - RiboJ-Uni RBS - Harmonized GFP - WT T7 terminator</li>
+
                                <li>L1_E: <a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918037">Harmonized GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>L1_F: 0.5 T7 promoter - SarJ-Uni RBS- TALEsp1 - WT T7 terminator variant</li>
+
                                <li>L1_F: <a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a> - <a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li>
−
                        <li>L1_G: 0.1 T7 promoter - SarJ-Uni RBS - TALEsp1- WT T7 terminator</li>
+
                                <li>L1_G: <a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a>- <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>L1_H: 0.5 T7 promoter - RiboJ-Uni RBS - Harmonized GFP - WT T7 termniator</li>
+
                                <li>L1_H: <a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918037">Harmonized GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li>
−
                      </ul>
+
                            </ul>  
−
                      <br>
+
                            <br>
−
                      The following multiple transcriptional units were assembled in MoClo level M backbones:
+
                            <p>The following multiple transcriptional units were assembled in MoClo level M backbones:</p>
−
                      <ul>
+
−
                        <li>L1_C+L1_A </li>
+
−
                        <li>L1_D+L1_B </li>
+
−
                        <li>L1_D+L1_E </li>
+
−
                        <li>L1_F+L1_H </li>
+
−
                        <li>L1_G+L1_B </li>
+
  
−
                      </ul>
+
                            <ul>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918050">L1_C+L1_A</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918046">L1_D+L1_B</a></li>
 +
                                <li><a href="#">L1_D+L1_E</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918048">L1_F+L1_H</a></li>
 +
                                <li><a href="http://parts.igem.org/Part:BBa_K2918040">L1_G+L1_B</a></li>
 +
                            </ul>
  
 
                     </td></tr>
 
                     </td></tr>

Revision as of 22:18, 21 October 2019

Sci-Phi 29

Orthogonal Replication


Cloning Testing of Plasmid
April
May
June
July
August
September
October


Controllable Expression


Cloning Testing of Plasmid
April
May
June
July
August
September
October