Difference between revisions of "Team:UM Macau/Public Engagement"

 
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    <h1>Demonstrate</h1>
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<h1>Education & Public Engagement</h1>
 
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<p>The internal and external parts of our team collaborated to design and complete the tests and analysis to generate a practical and comprehensive demonstration for our project. The tests included two parts, realistic water test and bacteria viability test. We met some barriers in the process such as unexpected experiment results, obstacles in water samples acquisition, and limitation of time. We managed to break the barriers and bring about some convincing results. After analysis, we believe that our project can function well and be applied in the real situation. </p>
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<p>In our project, we conducted public engagement activities to achieve the goal of abridging synthetic biology to the public. Considering that people from different backgrounds have different pre-existing knowledge and interests in absorbing new information, we designed our activities in three levels referring to the education level of targeting population, with regards to middle/high school students, undergraduate students, and the general public. Different activities were designed based on their backgrounds in the formats of workshops, summer camps, lectures, and interviews respectively. By conducting the activities, we were able to spread the basic knowledge of synthetic biology with the example of how we adopted it in constructing OPHT protein complex for nanoparticle elimination. Besides, we were able to raise awareness of the public in the importance of protecting water resources. The goals of appealing more attention from the public and addressing potential effects of nanoparticle pollution were also achieved. </p>
 
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   <p>We chose two incubation time points: 6 minutes and 48 minutes to plot the graph. </p>
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   <p>We conducted the DIY water purification kit workshop in two local high schools, Hou Kong High School and Sam Yuk Middle School, the two of the best secondary schools in Macau. The activity helped to enrich local students’ knowledge of water pollution as well as nanoparticle pollution and helped to raise their awareness on protecting water resources and get interested in scientific innovations. The workshop contained two parts, an introduction to water pollution, and interesting experiments of dirty water purification. The basic water purification mechanisms were learned and comprehended by students in the progress of making their own kit.</p>
<p> In sample A obtained from Inner Harbour, we can see the concentration of nanoparticles captured by OPHT2 bacteria and wild-type bacteria has significant differences between each other. This river water sample contains heavy metals molecules and unknown dirty particles which might occupy the nanoparticles binding sites on the sticky protein of our bacteria SANCE. In terms of different time points incubation, OPHT2 shown able to capture more nanoparticles after 48 minutes incubation compared to 6 minutes. From Figure 2A, in fact we can see the concentration of nanoparticle captured by OPHT2 after 6 minutes and 48 minutes do not change, the significant increases due to the decrease of nanoparticle captured by wild-type bacteria after longer incubation time. Therefore, we can conclude that OPHT2 bacteria is able to capture nanoparticles even under the water sample A’s harsh environment due to the fact that there are significant differences in both time points. </p>
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<p>In sample B which is a reservoir used to store water, Figure 2B shows that the nanoparticle concentration differences between OPHT2 and wild-type are both significant at 6 and 48 minutes incubation, indicating that our OPHT2 construct works well in sample B. From another viewpoint, the data is more significant under 6 minutes incubation compared to 48 minutes. Along with the increase of incubation time, the nanoparticle binding sites are gradually occupied. Thus, the concentration of nanoparticle captured decreases after 48 minutes incubation. As there are significant differences in both time points, we can conclude that our bacteria with OPHT2 construct works in water sample B environment.</p>
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<p>From Figure 2C, it is shown that our bacteria SANCE performed the best capturing ability in sample C which is from the Outer Harbour. The nanoparticle concentration captured by OPHT2 bacteria is way higher than wild-type bacteria at 6 minutes incubation. At 48 minutes incubation time point, the significance decreases compared to the 6 minutes time point as the protein binding sites for nanoparticles will reach a saturation point along with the increase of incubation time. As this water source is from lake water, it makes sense that our bacteria SANCE can perform its sticky function normally in a cleaner water environment which has related normal pH and stable condition for bacteria in comparison to sample A and B. Therefore, it can be concluded that our bacteria able to capture nanoparticles in water sample C.</p>
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<p>Based on the results above, we can safely conclude that our SANCE is able to perform similarly in realistic water conditions.</p>
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<p>During our interview to Hong Kong experts, one of the interviewees from The University of Hong Kong, Professor Leung, Kenneth Mei Yee raised a concern that toxicity of nanoparticles might be harmful and powerful to kill our bacteria organisms. Therefore, we designed a survival test to investigate whether toxic nanoparticles can actually kill our bacteria. We have chosen two nanoparticles which is AgNP (20nm in size) and ZnO (30±10nm) respectively. 5 different concentrations of Zinc Oxide and Silver nanoparticles were prepared in LB broth solution with concentrations of  0.5 mg/L, 1 mg/L, 20 mg/L, 40 mg/L and 60 mg/L respectively. We chose this range because according to literature, the average concentration of AgNP in waste water treatment plants is 1 mg/L. </p>
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<p><img src="https://static.igem.org/mediawiki/2019/0/0e/T--UM_Macau--seamarket2.jpeg" style="float:right; width:40%; margin-left: 3%; margin-top: 1%;">Many studies claimed that nanoparticles in the marine environment can be absorbed by living organisms which later might become the dishes on the table of local residents and potentially influence their health. Seafood product sellers in markets are the people with closest contacts to seafood products. In order to investigate whether nanoparticles would affect the quality of seafood in Macau, and collect the understanding in nanoparticle pollution from the local residents, we arranged and conducted local market interviews targeting sellers and customers in Macau local seafood markets, and 29 valid replies were collected.</p>
 
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      <p>Synthetic biology is still a quite mysterious area for students who still study in high school or middle school. With this concern, we got involved in the high school summer camp as teacher assistants to enrich their knowledge of synthetic biology. The high school students who attended UM summer camp were invited to participate in the lecture on introducing nanoparticle pollution and synthetic biology. After the lecture, the students showed their awareness of nanoparticle pollution and raised some good questions.</p>
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Latest revision as of 20:24, 21 October 2019


Education & Public Engagement

Overview

In our project, we conducted public engagement activities to achieve the goal of abridging synthetic biology to the public. Considering that people from different backgrounds have different pre-existing knowledge and interests in absorbing new information, we designed our activities in three levels referring to the education level of targeting population, with regards to middle/high school students, undergraduate students, and the general public. Different activities were designed based on their backgrounds in the formats of workshops, summer camps, lectures, and interviews respectively. By conducting the activities, we were able to spread the basic knowledge of synthetic biology with the example of how we adopted it in constructing OPHT protein complex for nanoparticle elimination. Besides, we were able to raise awareness of the public in the importance of protecting water resources. The goals of appealing more attention from the public and addressing potential effects of nanoparticle pollution were also achieved.

Synthetic biology is one of the main methods adopted in solving biological issues. Applying the synthetic biology techniques in realistic problems is always the core of iGEM. Conducting the residential college lecture in university is one of the best ways of introducing synthetic biology to the general public. As an iGEM team formed by biomedical majored undergraduate students, we felt obliged in presenting and introducing synthetic biology to reveal its benefits and its affable face to university students. We held a residential college lecture in Ma Man Kei and Lo Pak Sam college at the University of Macau. By this chance, we also introduced nanoparticle pollution and our project as one potential solution. Students were interested in SANCE and actively in asking questions. Not only the students, the vice-rector of the University of Macau also found our project appealing and meaningful.



We conducted the DIY water purification kit workshop in two local high schools, Hou Kong High School and Sam Yuk Middle School, the two of the best secondary schools in Macau. The activity helped to enrich local students’ knowledge of water pollution as well as nanoparticle pollution and helped to raise their awareness on protecting water resources and get interested in scientific innovations. The workshop contained two parts, an introduction to water pollution, and interesting experiments of dirty water purification. The basic water purification mechanisms were learned and comprehended by students in the progress of making their own kit.



Many studies claimed that nanoparticles in the marine environment can be absorbed by living organisms which later might become the dishes on the table of local residents and potentially influence their health. Seafood product sellers in markets are the people with closest contacts to seafood products. In order to investigate whether nanoparticles would affect the quality of seafood in Macau, and collect the understanding in nanoparticle pollution from the local residents, we arranged and conducted local market interviews targeting sellers and customers in Macau local seafood markets, and 29 valid replies were collected.


The sea products they sell mostly come from the ocean potentially exposing to nanoparticle contamination. Many sellers have more than 20 years of experience, but they claimed that the quality and amount of sea product didn’t change within these 2 decades. After further inquiry, we found that this resulted from the reason that most of them have no idea that the quality of seafood products could be affected by water pollution, let alone the nanoparticle pollution. This result suggested that the potential hazard of nanoparticle pollution hasn’t been fully recognized and focused by local residents. By holding this local market interview, we were able to bring new perspectives on water pollution as well as the suggestions on seafood safety to local sellers and customers.


Synthetic biology is still a quite mysterious area for students who still study in high school or middle school. With this concern, we got involved in the high school summer camp as teacher assistants to enrich their knowledge of synthetic biology. The high school students who attended UM summer camp were invited to participate in the lecture on introducing nanoparticle pollution and synthetic biology. After the lecture, the students showed their awareness of nanoparticle pollution and raised some good questions.



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