Team:UM Macau/Part Collection


Part Collection

Basic Parts


Part Number Name Type Description Length(bp)
BBa_K3033000 T7 promoter Regulatory Derived from pET11a vector; IPTG inducible promoter for protein expression. 19
BBa_K3033001 Arabad promoter Regulatory Derived from pBAD24 vector; Arabinose inducible promoter for protein expression 285
BBa_K3033002 Ribosome binding site (Pet11a) RBS Derived from pET11a vector; ribosome binding site for protein translation 23
BBa_K3033003 Ribosome binding site (pBAD24) RBS Derived from pBAD24 vector; ribosome binding site for protein translation 31
BBa_K3033004 Outer membrane protein W Coding Derived from E.coli; sequence was extracted from NCBI. It is for carrying our adhesion system to the surface of E.coli 573
BBa_K3033005 Perhydrolase Coding Derived from Pseudomonas aeruginosa; sequence was extracted from NCBI. It enhances the function of our adhesion system through epoxidation. 828
BBa_K3033006 6 Histidine tag Tag This tag was used for protein detection through western blotting and protein purification 18
BBa_K3033007 2-Tyrosine Coding This codes for the two tyrosine at the end of our adhesion system 6
BBa_K3033008 6-Tyrosine Coding This codes for the six tyrosine at the end of our adhesion system. 18
BBa_K3033009 eGFP Coding This codes for the green fluorescent protein which is used to tag our fusion protein for us to see its localization 717
BBa_K3033010 Mefp-5 Coding This codes for the protein Mefp-5 which would be displayed on the surface of our E.coli 297
BBa_K3033011 2-Asp Coding This codes for two aspartic acid at the end of our capturing system. 6
BBa_K3033012 NSP4 Signaling Derived from E.coli. This codes for a secretion signal sequence which would help our tyrosinase enzyme to be transported into the medium 60
BBa_K3033013 Tyrosinase Coding Derived from bacillus megaterium. This codes for the enzyme which would be secreted into the extracellular matrix 891
BBa_K3033014 (Characterized Part) FtnA Coding Derived from E.coli. This codes for the ferratin which would be storing ferric ions for our magnetization function 516
BBa_K3033015 T7 terminator Terminator Derived from pet11a. This codes for a T7 terminator site. 48
BBa_K3033016 rrnB T1 Terminator Terminator Derived from pBAD24. This codes for a rrnB T1 Terminator site. 87
BBa_K3033017 Flag tag Tag For the checking of protein expression. 24

Composite Parts


Part Number Name Component Parts Description Length(bp)
BBa_K3033018 OPHT Capture system version 1: 2-Tyrosine capturing domain BBa_K3033000, BBa_K3033002, BBa_K3033004,BBa_K3033005,BBa_K3033006, BBa_K3033007, BBa_K3033015 Surface capture system variant which contains 2-Tyrosine for its capturing domain. 1643
BBa_K3033019 OPHTII Capture system version 2: eGFP tagged at the N-terminal 2-Tyrosine capturing domain BBa_K3033000, BBa_K3033002, BBa_K3033009, BBa_K3033004,BBa_K3033005,BBa_K3033006, BBa_K3033007, BBa_K3033015 Surface capture system variant which contains eGFP tag at the N-terminal and 2-Tyrosine for its capturing domain. 2360
BBa_K3033020 OPHTIII Capture system version 3: 6-Tyrosine capturing domain BBa_K3033000, BBa_K3033002, BBa_K3033009, BBa_K3033004, BBa_K3033005, BBa_K3033006, BBa_K3033007, BBa_K3033015 Surface capture system variant which contains 6-Tyrosine for its capturing domain. 1655
BBa_K3033021 OPHM Capture system version 4: Mefp-5 capturing domain. BBa_K3033000, BBa_K3033002, BBa_K3033004,BBa_K3033005,BBa_K3033006, BBa_K3033010, BBa_K3033015 Surface capture system variant which contains Mefp-5 for its capturing domain. 1931
BBa_K3033022 OPHA Capture system version 5: 2-Aspartic acid capturing domain BBa_K3033000, BBa_K3033002, BBa_K3033004,BBa_K3033005,BBa_K3033006, BBa_K3033011, BBa_K3033015 Surface capture system variant which contains 2-Aspartic acid for its capturing domain 1643
BBa_K3033023 (Validated Part) NSP4_Tyrosinase Secretable tyrosinase system BBa_K3033001, BBa_K3033003, BBa_K3033012,BBa_K3033013,BBa_K3033006, BBa_K3033016 Secretable tyrosinase enyzme with NSP4 as the secretion signal 1825
BBa_K3033024 FtnA overexpression BBa_K3033001, BBa_K3033003, BBa_K3033014,BBa_K3033017, BBa_K3033016 Overexpression part of the FtnA part 1152

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