Team:UM Macau/Part Collection
Part Collection
Basic Parts
| Part Number | Name | Type | Description | Length(bp) |
|---|---|---|---|---|
| BBa_K3033000 | T7 promoter | Regulatory | Derived from pET11a vector; IPTG inducible promoter for protein expression. | 19 |
| BBa_K3033001 | Arabad promoter | Regulatory | Derived from pBAD24 vector; Arabinose inducible promoter for protein expression | 285 |
| BBa_K3033002 | Ribosome binding site (Pet11a) | RBS | Derived from pET11a vector; ribosome binding site for protein translation | 23 |
| BBa_K3033003 | Ribosome binding site (pBAD24) | RBS | Derived from pBAD24 vector; ribosome binding site for protein translation | 31 |
| BBa_K3033004 | Outer membrane protein W | Coding | Derived from E.coli; sequence was extracted from NCBI. It is for carrying our adhesion system to the surface of E.coli | 573 |
| BBa_K3033005 | Perhydrolase | Coding | Derived from Pseudomonas aeruginosa; sequence was extracted from NCBI. It enhances the function of our adhesion system through epoxidation. | 828 |
| BBa_K3033006 | 6 Histidine tag | Tag | This tag was used for protein detection through western blotting and protein purification | 18 |
| BBa_K3033007 | 2-Tyrosine | Coding | This codes for the two tyrosine at the end of our adhesion system | 6 |
| BBa_K3033008 | 6-Tyrosine | Coding | This codes for the six tyrosine at the end of our adhesion system. | 18 |
| BBa_K3033009 | eGFP | Coding | This codes for the green fluorescent protein which is used to tag our fusion protein for us to see its localization | 717 |
| BBa_K3033010 | Mefp-5 | Coding | This codes for the protein Mefp-5 which would be displayed on the surface of our E.coli | 297 |
| BBa_K3033011 | 2-Asp | Coding | This codes for two aspartic acid at the end of our capturing system. | 6 |
| BBa_K3033012 | NSP4 | Signaling | Derived from E.coli. This codes for a secretion signal sequence which would help our tyrosinase enzyme to be transported into the medium | 60 |
| BBa_K3033013 | Tyrosinase | Coding | Derived from bacillus megaterium. This codes for the enzyme which would be secreted into the extracellular matrix | 891 |
| BBa_K3033014 (Characterized Part) | FtnA | Coding | Derived from E.coli. This codes for the ferratin which would be storing ferric ions for our magnetization function | 516 |
| BBa_K3033015 | T7 terminator | Terminator | Derived from pet11a. This codes for a T7 terminator site. | 48 |
| BBa_K3033016 | rrnB T1 Terminator | Terminator | Derived from pBAD24. This codes for a rrnB T1 Terminator site. | 87 |
| BBa_K3033017 | Flag tag | Tag | For the checking of protein expression. | 24 |
Composite Parts
| Part Number | Name | Component Parts | Description | Length(bp) |
|---|---|---|---|---|
| BBa_K3033018 | OPHT Capture system version 1: 2-Tyrosine capturing domain | BBa_K3033000, BBa_K3033002, BBa_K3033004,BBa_K3033005,BBa_K3033006, BBa_K3033007, BBa_K3033015 | Surface capture system variant which contains 2-Tyrosine for its capturing domain. | 1643 |
| BBa_K3033019 | OPHTII Capture system version 2: eGFP tagged at the N-terminal 2-Tyrosine capturing domain | BBa_K3033000, BBa_K3033002, BBa_K3033009, BBa_K3033004,BBa_K3033005,BBa_K3033006, BBa_K3033007, BBa_K3033015 | Surface capture system variant which contains eGFP tag at the N-terminal and 2-Tyrosine for its capturing domain. | 2360 |
| BBa_K3033020 | OPHTIII Capture system version 3: 6-Tyrosine capturing domain | BBa_K3033000, BBa_K3033002, BBa_K3033009, BBa_K3033004, BBa_K3033005, BBa_K3033006, BBa_K3033007, BBa_K3033015 | Surface capture system variant which contains 6-Tyrosine for its capturing domain. | 1655 |
| BBa_K3033021 | OPHM Capture system version 4: Mefp-5 capturing domain. | BBa_K3033000, BBa_K3033002, BBa_K3033004,BBa_K3033005,BBa_K3033006, BBa_K3033010, BBa_K3033015 | Surface capture system variant which contains Mefp-5 for its capturing domain. | 1931 |
| BBa_K3033022 | OPHA Capture system version 5: 2-Aspartic acid capturing domain | BBa_K3033000, BBa_K3033002, BBa_K3033004,BBa_K3033005,BBa_K3033006, BBa_K3033011, BBa_K3033015 | Surface capture system variant which contains 2-Aspartic acid for its capturing domain | 1643 |
| BBa_K3033023 (Validated Part) | NSP4_Tyrosinase Secretable tyrosinase system | BBa_K3033001, BBa_K3033003, BBa_K3033012,BBa_K3033013,BBa_K3033006, BBa_K3033016 | Secretable tyrosinase enyzme with NSP4 as the secretion signal | 1825 |
| BBa_K3033024 | FtnA overexpression | BBa_K3033001, BBa_K3033003, BBa_K3033014,BBa_K3033017, BBa_K3033016 | Overexpression part of the FtnA part | 1152 |