Difference between revisions of "Team:EPFL/Experiments"
| Line 162: | Line 162: | ||
</div> | </div> | ||
</div> | </div> | ||
| − | + | <div class="col-md-12"> | |
<!--**************CELL-FREE SYSTEM**************--> | <!--**************CELL-FREE SYSTEM**************--> | ||
Revision as of 15:37, 21 October 2019
Protocols
General Protocols
Agarose Gel Electrophoresis
SDS-Page Protein electrophoresis
Transformation
DNA Extraction
Microneedle Patches Fabrication
DNA extraction
RPA Amplification
RPA from banana DNA
RPA Amplification with Agdia AmplifyRP® kit
RPA Amplification with TwistAmp kit
Toeholds
Toehold switch assembly
Toehold testing
Toehold plate reader settings
Signal Generation
2-step PCR
CDO Expression in a cell-free system
Cell-Free Systems
Protein Purification
Ribosome Purification
Buffer preparation
Energy Solution preparation