Difference between revisions of "Team:EPFL/Experiments"
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<div class="col-md-2"> | <div class="col-md-2"> | ||
| − | <div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.7zrhp56" target="_blank" ><img src="https://static.igem.org/mediawiki/2019/ | + | <div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.7zrhp56" target="_blank" ><img src="https://static.igem.org/mediawiki/2019/b/bd/T--EPFL--Signal_icon.png" alt=""></a> |
<h6>2-step PCR</h6> | <h6>2-step PCR</h6> | ||
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<h2 class="text-special text-center m-b-40" style="color:purple">Cell-Free System</h2> | <h2 class="text-special text-center m-b-40" style="color:purple">Cell-Free System</h2> | ||
<div class="row"><!--**************First Row**************--> | <div class="row"><!--**************First Row**************--> | ||
| + | <div class="col-md-2"> | ||
| + | <div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.5bbg2in" target="_blank"><img src="https://static.igem.org/mediawiki/2019/f/ff/T--EPFL--OnePot_icon.png" alt=""></a> | ||
| + | <h6>Protein Purification</h6> | ||
| + | </div> | ||
| + | </div> | ||
<div class="col-md-2"> | <div class="col-md-2"> | ||
<div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.5bbg2in" target="_blank"><img src="https://static.igem.org/mediawiki/2019/f/ff/T--EPFL--OnePot_icon.png" alt=""></a> | <div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.5bbg2in" target="_blank"><img src="https://static.igem.org/mediawiki/2019/f/ff/T--EPFL--OnePot_icon.png" alt=""></a> | ||
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| − | <div class="col-md-2"> | + | <div class="col-md-2"> |
<div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.8ayhsfw" target="_blank"><img src="https://static.igem.org/mediawiki/2019/f/ff/T--EPFL--OnePot_icon.png" alt=""></a> | <div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.8ayhsfw" target="_blank"><img src="https://static.igem.org/mediawiki/2019/f/ff/T--EPFL--OnePot_icon.png" alt=""></a> | ||
<h6>Energy Solution preparation</h6> | <h6>Energy Solution preparation</h6> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <div class="col-md-2"> | ||
| + | <div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.8avhse6" target="_blank"><img src="https://static.igem.org/mediawiki/2019/f/ff/T--EPFL--OnePot_icon.png" alt=""></a> | ||
| + | <h6>Protein expression</h6> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
</div> | </div> | ||
Revision as of 11:25, 21 October 2019
Protocols
General Protocols
Agarose Gel Electrophoresis
SDS-Page Protein electrophoresis
Transformation
DNA Extraction
Microneedle Patches Fabrication
DNA extraction
RPA Amplification
RPA from banana DNA
RPA Amplification with Agdia AmplifyRP® kit
RPA Amplification with TwistAmp kit
Toeholds
Toehold switch assembly
Toehold testing
Toehold plate reader settings
Signal Generation
2-step PCR
CDO Expression in a cell-free system
Cell-Free System
Protein Purification
Ribosome Purification
Buffer preparation
Energy Solution preparation