Difference between revisions of "Team:EPFL/Experiments"
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| − | <div class="demo-item"><a href=" | + | <div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.8cchssw" target="_blank"><img src="https://static.igem.org/mediawiki/2019/4/42/T--EPFL--DNAextraction_icon.png" alt=""></a> |
| − | <h6>Microneedle Fabrication</h6> | + | <h6>Microneedle Patches Fabrication</h6> |
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| − | <div class="demo-item"><a href=" | + | <div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.8ephtdn" target="_blank"><img src="https://static.igem.org/mediawiki/2019/a/ad/T--EPFL--RPA_icon.png" alt=""></a> |
| − | <h6> | + | <h6>RPA Amplification with Agdia AmplifyRP® kit</h6> |
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| − | <div class="demo-item"><a href=" | + | <div class="demo-item"><a href="https://dx.doi.org/10.17504/protocols.io.8erhtd6"><img src="https://static.igem.org/mediawiki/2019/a/ad/T--EPFL--RPA_icon.png" alt=""></a> |
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<div class="demo-item"><a href="https://www.protocols.io/view/toehold-testing-pure-system-protein-production-73ehqje"><img src="https://static.igem.org/mediawiki/2019/a/a6/T--EPFL--Toehold_icon.png""></a> | <div class="demo-item"><a href="https://www.protocols.io/view/toehold-testing-pure-system-protein-production-73ehqje"><img src="https://static.igem.org/mediawiki/2019/a/a6/T--EPFL--Toehold_icon.png""></a> | ||
| − | <h6>Toehold | + | <h6>Toehold testing</h6> |
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Revision as of 10:03, 21 October 2019
Protocols
General Protocols
2-step PCR
Agarose Gel Electrophoresis
SDS-Page Protein electrophoresis
Transformation
DNA Extraction
Microneedle Patches Fabrication
DNA extraction
RPA Amplification
RPA from banana DNA
RPA Amplification with Agdia AmplifyRP® kit
RPA Amplification with TwistAmp kit
Toeholds
Toehold switch assembly
Toehold testing
Toehold plate reader settings
Signal Generation
sfGFP gene assembly
CDO gene
CDO Expression in a cell-free system
Cell-Free System
Ribosome Purification
Buffer preparation