Difference between revisions of "Team:TUDelft/PartsTest"

Line 315: Line 315:
 
                                  
 
                                  
  
−
                                 <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53816"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918009">BBa_K2918009</a></td><td>Regulatory</td><td>0.5 T7 promoter variant with TALEsp1 binding site</td><td width="100px">TUDelft 2019</td><td align="right">41</td>
+
                                 <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53816"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918009">BBa_K2918009</a></td><td>Regulatory</td><td>0.5 T7sp1 promoter </td><td width="100px">TUDelft 2019</td><td align="right">41</td>
  
−
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54192"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918035">BBa_K2918035</a></td><td>RBS</td><td>BBa_B0032 RBS and RiboJ</td><td width="100px">TUDelft 2019</td><td align="right">94</td>
+
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54192"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918035">BBa_K2918035</a></td><td>5'UTR</td><td>BBa_B0032 RBS and RiboJ</td><td width="100px">TUDelft 2019</td><td align="right">94</td>
  
−
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54374"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918013">BBa_K2918013</a></td><td>RBS</td><td>BBa_B0032 RBS and SarJ</td><td width="100px">TUDelft 2019</td><td align="right">98</td>
+
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54374"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918013">BBa_K2918013</a></td><td>5'UTR</td><td>BBa_B0032 RBS and SarJ</td><td width="100px">TUDelft 2019</td><td align="right">98</td>
  
−
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53815"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>&hearts;</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918034">BBa_K2918034</a></td><td>Regulatory</td><td>Broad host range promoter</td><td width="100px">TUDelft 2019</td><td align="right">56</td>
+
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53815"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>&hearts;</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918034">BBa_K2918034</a></td><td>Regulatory</td><td>Broad Host Range Promoter (P<sub>BHR</sub>)</td><td width="100px">TUDelft 2019</td><td align="right">56</td>
  
−
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54383"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918011">BBa_K2918011</a></td><td>Regulatory</td><td>Broad host range promoter with TALEsp1 binding site</td><td width="100px">TUDelft 2019</td><td align="right">56</td>
+
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54383"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918011">BBa_K2918011</a></td><td>Regulatory</td><td>(P<sub>BHR</sub>sp1)</td><td width="100px">TUDelft 2019</td><td align="right">56</td>
  
  
−
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55155"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918038">BBa_K2918038</a></td><td>RBS</td><td>RiboJ and universal binding site</td><td width="100px">TUDelft 2019</td><td align="right">85</td>
+
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55155"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918038">BBa_K2918038</a></td><td>5'UTR</td><td>Universal RBS and RiboJ </td><td width="100px">TUDelft 2019</td><td align="right">85</td>
  
−
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55150"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918012">BBa_K2918012</a></td><td>Other</td><td>Ribozyme J (Ribo J)</td><td width="100px">TUDelft 2019</td><td align="right">75</td>
+
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55150"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918012">BBa_K2918012</a></td><td>Ribozyme</td><td>Ribo J</td><td width="100px">TUDelft 2019</td><td align="right">75</td>
  
−
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54379"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918036">BBa_K2918036</a></td><td>RBS</td><td>SarJ and universal ribosome binding site </td><td width="100px">TUDelft 2019</td><td align="right">89</td>
+
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54379"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918036">BBa_K2918036</a></td><td>5'UTR</td><td>SarJ and Universal RBS</td><td width="100px">TUDelft 2019</td><td align="right">89</td>
  
−
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54352"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918010">BBa_K2918010</a></td><td>Regulatory</td><td>T7 promoter with TALEsp1 binding site</td><td width="100px">TUDelft 2019</td><td align="right">41</td>
+
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54352"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918010">BBa_K2918010</a></td><td>Regulatory</td><td>T7sp1</td><td width="100px">TUDelft 2019</td><td align="right">41</td>
  
 
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54349"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918016">BBa_K2918016</a></td><td>Terminator</td><td>T7 terminator variant</td><td width="100px">TUDelft 2019</td><td align="right">48</td>
 
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54349"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918016">BBa_K2918016</a></td><td>Terminator</td><td>T7 terminator variant</td><td width="100px">TUDelft 2019</td><td align="right">48</td>
Line 340: Line 340:
 
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54344"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918014">BBa_K2918014</a></td><td>RBS</td><td>Universal RBS</td><td width="100px">TUDelft2019</td><td align="right">10</td>
 
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54344"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918014">BBa_K2918014</a></td><td>RBS</td><td>Universal RBS</td><td width="100px">TUDelft2019</td><td align="right">10</td>
  
−
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53929"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918062">BBa_K2918062</a></td><td>RBS</td><td>Universal RBS site adapted for modular cloning with ribozymes</td><td width="100px">TUDelft 2019</td><td align="right">16</td>
+
                              
−
 
+
  <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54344"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918062">BBa_K2918062</a></td><td>Composite</td><td>TALEsp1 stablized P<sub>BHR</sub> promoter</td><td width="100px">TUDelft2019</td><td align="right">10</td>
 
                          
 
                          
  
Line 352: Line 352:
 
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>&hearts;</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918001">BBa_K2918001</a></td><td>Coding</td><td>Φ29 Terminal Protein (TP/p3)</td><td width="100px">TUDelft2019</td><td align="right">801</td>
 
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>&hearts;</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918001">BBa_K2918001</a></td><td>Coding</td><td>Φ29 Terminal Protein (TP/p3)</td><td width="100px">TUDelft2019</td><td align="right">801</td>
  
−
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>&hearts;</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918033">BBa_K2918033</a></td><td>Coding</td><td>Φ29 Left origin of replication (Ori-L)</td><td width="100px">TUDelft2019</td><td align="right">801</td>
+
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>&hearts;</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918033">BBa_K2918033</a></td><td>ORI</td><td>Φ29 Left origin of replication (Ori-L)</td><td width="100px">TUDelft2019</td><td align="right">801</td>
  
−
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>&hearts;</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918061">BBa_K2918061</a></td><td>Coding</td><td>Φ29 Right origin of replication (Ori-R)</td><td width="100px">TUDelft2019</td><td align="right">801</td>
+
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>&hearts;</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918061">BBa_K2918061</a></td><td>ORI</td><td>Φ29 Right origin of replication (Ori-R)</td><td width="100px">TUDelft2019</td><td align="right">801</td>
  
−
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>&hearts;</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918039">BBa_K2918039</a></td><td>Coding</td><td>SarJ</td><td width="100px">TUDelft2019</td><td align="right">801</td>
+
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>&hearts;</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918039">BBa_K2918039</a></td><td>Ribozyme</td><td>SarJ</td><td width="100px">TUDelft2019</td><td align="right">801</td>
  
 
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53857"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr></tbody></table>                               
 
                             <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&amp;group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53857"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr></tbody></table>                               

Revision as of 20:32, 17 October 2019

Sci-Phi 29

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  • Our Best Basic Part﹀
    ♥ Name Description
    ♥ BBa_K2918000 Φ29 DNA polymerase (DNAP/p2)

    Hello

  • Our Best Composite Part﹀
    ♥ Name Description Designer
    ♥ BBa_K2918000 blablablablablablablablablablablabla TU Delft 2019
  • Our Part Collection﹀

    Engineering non-model organisms requires discovery of organism-specific parts or re-tuning of parts characterized in model organisms. Our part collection provides modular cloning (MoClo) compatible parts that can be used for predictable expression of genes across different bacterial species.

    Our collections provides essential parts to overcome the need to identify parts for different bacterial hosts and insulate the parts from variations associated with microbial context. Our toolkit offers essential tools for effortless engineering of a range of bacterial species.


    ori

    The process of identification of origins of replication for different bacterial species is laborious and broad host range plasmids, due to their size, are a resource burden to the bacterial host (Jain and Srivastava 2013). Our toolkit provides all the components of the Phi29 replication machinery necessary to establish host independent replication.


    promoter

    Non-model bacterial species have a limited collection of promoters (Yang, Liu et al. 2018) . Our toolkit provides T7 promoter variants of different strengths which in combination with a T7 RNA polymerase mixed forward loop (UBER,hyperlink to design page), can be used for gene expression across different bacterial species. In addition, the collection consists of a broad host range promoter (Pbhr) based on conserved regions of -10 box and -35 box sequences in E.coli and B.subtilis (Yang, Liu et al. 2018) .


    ribozyme

    To achieve modularity and predictability of synthetic parts, insulation from their genetic context needs to be established. Sequences downstream of the transcription start site (TSS) of promoters end up in transcripts and are shown to significantly affect translational rates (Lou et al. 2012). Our toolkit consists of ribozymes which when included in 5’UTR sequences, due to their self cleavage properties, cleave any artifacts left behind in the transcripts upstream of the RBS.


    RBS

    Our toolkit contains an RBS (universal RBS) predicted to work (RBS calculator 2.0, link to www.denovodna.com) across a range of bacterial species.


    CDS

    Toolkit consists of coding sequence for eGFP generated using our cross-species codon harmonizer (2019.igem.org/Team:TUDelft/Model). This tool allows you to generate a coding sequence with equal codon usage across a range of organisms. eGFP sequence was predicted for equal codon usage in E.coli, B.subtilis and V.natriegens.


    terminator

    Toolkit consists of variants of T7 terminators. Use of these variants reduces chances of homologous recombination between transcriptional units.


    TALE

    Behaviour of parts is influenced by microbial context dependent variations (eg. copy number, transcription rates and translation rates) and to overcome the need to re-characterize parts for different bacterial species, an incoherent feed forward loop (iFFL) can be implemented in a genetic circuit (Segall-Shapiro et al. 2018). To establish an iFFL genetic circuit, TALE (Transcriptional Activator Like Effector molecule) proteins can be used as repressors. Our toolkit contains TALE protein designed to target a specific DNA sequence. Furthermore, we engineered a series of promoters that can be targeted by the TALE protein.

    Part Collection

    ♥NameTypeDescriptionDesignerLength
    BBa_K2918005Regulatory0.1 T7 promoter TUDelft201923
    BBa_K2918006Regulatory0.5 T7 promoterTUDelft201923
    BBa_K2918009Regulatory0.5 T7sp1 promoter TUDelft 201941
    BBa_K29180355'UTRBBa_B0032 RBS and RiboJTUDelft 201994
    BBa_K29180135'UTRBBa_B0032 RBS and SarJTUDelft 201998
    ♥BBa_K2918034RegulatoryBroad Host Range Promoter (PBHR)TUDelft 201956
    BBa_K2918011Regulatory(PBHRsp1)TUDelft 201956
    BBa_K29180385'UTRUniversal RBS and RiboJ TUDelft 201985
    BBa_K2918012RibozymeRibo JTUDelft 201975
    BBa_K29180365'UTRSarJ and Universal RBSTUDelft 201989
    BBa_K2918010RegulatoryT7sp1TUDelft 201941
    BBa_K2918016TerminatorT7 terminator variantTUDelft 201948
    BBa_K2918008CodingTranscription Activator like Effector protein (TALEsp1)TUDelft20192640
    BBa_K2918014RBSUniversal RBSTUDelft201910
    BBa_K2918062CompositeTALEsp1 stablized PBHR promoterTUDelft201910
    ♥BBa_K2918000CodingΦ29 DNA polymerase (DNAP/p2)TUDelft20191719
    BBa_K2918003CodingΦ29 Double Strand Binding Protein (DSB/p6)TUDelft2019315
    BBa_K2918002CodingΦ29 Single Stranded Binding Protein (SSB/p5)TUDelft2019375
    ♥BBa_K2918001CodingΦ29 Terminal Protein (TP/p3)TUDelft2019801
    ♥BBa_K2918033ORIΦ29 Left origin of replication (Ori-L)TUDelft2019801
    ♥BBa_K2918061ORIΦ29 Right origin of replication (Ori-R)TUDelft2019801
    ♥BBa_K2918039RibozymeSarJTUDelft2019801
  • All parts﹀
    ♥NameTypeDescriptionDesignerLength
    BBa_K2918005Regulatory0.1 T7 promoter TUDelft201923
    BBa_K2918006Regulatory0.5 T7 promoterTUDelft201923
    BBa_K2918007RegulatoryWild type T7 promoter TUDelft201923
    BBa_K2918009Regulatory0.5 T7 promoter variant with TALEsp1 binding siteTUDelft 201941
    BBa_K2918035RBSBBa_B0032 RBS and RiboJTUDelft 201994
    BBa_K2918013RBSBBa_B0032 RBS and SarJTUDelft 201998
    ♥BBa_K2918034RegulatoryBroad host range promoterTUDelft 201956
    BBa_K2918011RegulatoryBroad host range promoter with TALEsp1 binding siteTUDelft 201956
    BBa_K2918037CodingCross-species harmonized GFPTUDelft 2019678
    BBa_K2918061OtherRBS (B0032) adapted for modular cloning with RibozymesTUDelft 201925
    BBa_K2918038RBSRiboJ and universal binding siteTUDelft 201985
    BBa_K2918012OtherRibozyme J (Ribo J)TUDelft 201975
    BBa_K2918036RBSSarJ and universal ribosome binding site TUDelft 201989
    BBa_K2918010RegulatoryT7 promoter with TALEsp1 binding siteTUDelft 201941
    BBa_K2918016TerminatorT7 terminator variantTUDelft 201948
    BBa_K2918008CodingTranscription Activator like Effector protein (TALEsp1)TUDelft20192640
    BBa_K2918014RBSUniversal RBSTUDelft201910
    BBa_K2918062RBSUniversal RBS site adapted for modular cloning with ribozymesTUDelft 201916
    BBa_K2918015TerminatorWild type T7 terminatorTUDelft201948
    ♥BBa_K2918000CodingΦ29 DNA polymerase (DNAP/p2)TUDelft20191719
    BBa_K2918003CodingΦ29 Double Strand Binding Protein (DSB/p6)TUDelft2019315
    BBa_K2918002CodingΦ29 Single Stranded Binding Protein (SSB/p5)TUDelft2019375
    ♥BBa_K2918001CodingΦ29 Terminal Protein (TP/p3)TUDelft2019801
    BBa_K2918017Composite0.1 T7 promoter - Universal RBS - Φ29 9 TP - WT T7 terminatorTUDelft2019890
    BBa_K2918018Composite0.5 T7 promoter - Universal RBS - Φ29 TP - T7 terminatorTUDelft2019904
    ♥BBa_K2918019CompositeWT T7 promoter - Universal RBS - Φ29 TP - T7 terminatorTUDelft2019904
    BBa_K2918020Composite0.1 T7 promoter - Universal RBS - Φ29 DNAP - T7 terminatorTUDelft20191822
    BBa_K2918021Composite0.5 T7 promoter - Universal RBS - Φ29 DNAP - T7 terminatorTUDelft20191822
    BBa_K2918022CompositeWT T7 promoter - Universal RBS - Φ29 DNAP - WT T7 terminatorTUDelft20191899
    BBa_K2918023Composite0.1 T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminatorTUDelft2019519
    BBa_K2918024Composite0.5 T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminatorTUDelft2019519
    BBa_K2918025CompositeWT T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminatorTUDelft2019519
    BBa_K2918026Composite0.1 T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminatorTUDelft2019579
    BBa_K2918027Composite0.5 T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminatorTUDelft2019579
    BBa_K2918028CompositeWT T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminatorTUDelft2019579
    BBa_K2918030CompositeWT T7 promoter - Universal RBS - GFP - T7 terminatorTUDelft2019811
    BBa_K2918031Composite0.1 T7 promoter - Universal RBS - GFP - T7 terminatorTUDelft2019811
    BBa_K2918032Composite0.5 T7 promoter - Universal RBS - GFP - T7 terminatorTUDelft2019811