The experiments were based on Biobits Explorer¹, we adapted them to fit with our project. We started by expressing sfGFP into OnePot PURE which allowed us to teach them about protein expression, as well as cell-free systems. Then, we isolated DNA from banana and strawberry, this simple experiment taught them about DNA and cell lysis. The last experiment was to amplify the banana DNA by RPA, they learned how DNA amplification works and how RPA differs from PCR.

The feedback was very positive, the students were happy to have some independence in their experiments and to observe fluorescent proteins. It was also an enriching experience for us, as it was our first time working as a team and performing in front of an audience. It highlighted our strength and areas of improvement in presentation skills.