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</tr> | </tr> | ||
</table> | </table> | ||
+ | <h2>Predictable and Transferable Expression</h2> | ||
+ | <table id="tabletu" class="notebook"> | ||
+ | <tr id="category"> | ||
+ | <th style="background-color:transparent; color:black;"></th> | ||
+ | <th style="background-color:transparent; color:black;">Cloning</th> | ||
+ | <th style="background-color:transparent; color:black;" colspan="3">Testing of Plasmid</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="month">April</td> | ||
+ | <td></td> | ||
+ | <td colspan="3"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="month">May</td> | ||
+ | <td></td> | ||
+ | <td colspan="3"></td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="month">June</td> | ||
+ | <td class="modalTD" > | ||
+ | <!-- Trigger/Open The Modal --> | ||
+ | <button class="block modal-button" href="#myModal10">Uber Plasmid</button> | ||
+ | |||
+ | <!-- The Modal --> | ||
+ | <div id="myModal10" class="modal"> | ||
+ | |||
+ | <!-- Modal content --> | ||
+ | <div class="modal-content"> | ||
+ | <div class="modal-header"> | ||
+ | <span class="close">×</span> | ||
+ | <h2>Uber Plasmid</h2> | ||
+ | </div> | ||
+ | <div class="modal-body"> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>Goal:</td> | ||
+ | <td>Construction of the Uber Plasmid</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Protocols Used:</td> | ||
+ | <td> <a href="https://2019.igem.org/Team:TUDelft/Experiments">Nanodrop DNA quantification</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments">Plasmid Isolation</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments">Phusion PCR</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments">Restriction Digestion</a>, | ||
+ | <a href="https://2019.igem.org/Team:TUDelft/Experiments"><i>E.coli</i> Heat Shock Transformation</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments">Ligation</a>, | ||
+ | <a href="https://2019.igem.org/Team:TUDelft/Experiments">Colony Polymerase PCR (GoTaq)</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments"><i>E. coli</i> Glycerol Stock</a>, | ||
+ | <a href="https://2019.igem.org/Team:TUDelft/Experiments">DNA Gel Purification</a>, | ||
+ | <a href="https://2019.igem.org/Team:TUDelft/Experiments">DNA Gel Electrophoresis</a> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Plan:</td> | ||
+ | <td>To digest the UBER plasmid and insert a casette with GFP to make the UBER plasmid fluorescence.</td></tr> | ||
+ | <tr> | ||
+ | <td>Results: | ||
+ | </td> | ||
+ | <td>Check PCR did not give the right size on the gel.</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="modal-footer"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </td> | ||
+ | <td colspan="3"></td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="month">July</td> | ||
+ | |||
+ | <td></td> | ||
+ | <td colspan="3"></td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="month">August</td> | ||
+ | <td rowspan=3 class="modalTD"> | ||
+ | <!-- Trigger/Open The Modal --> | ||
+ | <button class="block modal-button" href="#myModal11">MoClo Assembly</button> | ||
+ | |||
+ | <!-- The Modal --> | ||
+ | <div id="myModal11" class="modal"> | ||
+ | |||
+ | <!-- Modal content --> | ||
+ | <div class="modal-content"> | ||
+ | <div class="modal-header"> | ||
+ | <span class="close">×</span> | ||
+ | <h2>MoClo Assembly</h2> | ||
+ | </div> | ||
+ | <div class="modal-body"> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>Goal:</td> | ||
+ | <td>Assembly all the different parts with MoClo</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Protocols Used:</td> | ||
+ | <td> <a href="https://2019.igem.org/Team:TUDelft/Experiments">Nanodrop DNA quantification</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments">Plasmid Isolation</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments">MoClo</a>, | ||
+ | <a href="https://2019.igem.org/Team:TUDelft/Experiments"><i>E.coli</i> Heat Shock Transformation</a>, | ||
+ | <a href="https://2019.igem.org/Team:TUDelft/Experiments">Colony Polymerase PCR (GoTaq)</a>, <a href="https://2019.igem.org/Team:TUDelft/Experiments"><i>E. coli</i> Glycerol Stock</a>, | ||
+ | <a href="https://2019.igem.org/Team:TUDelft/Experiments">DNA Gel Electrophoresis</a> | ||
+ | <a href="https://2019.igem.org/Team:TUDelft/Experiments">Sequencing</a> | ||
+ | <a href="https://2019.igem.org/Team:TUDelft/Experiments">Xgal Stock</a> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Plan:</td> | ||
+ | <td>For Predictable & Transferable Expression, first, all the level 0's were constructed and then assembled in level 1 to make the following constructs: | ||
+ | <ul> | ||
+ | <li>0.5 T7 p - SarJ + B0032 - TALEsp1 - WT T7 t</li> | ||
+ | <li>T7 p - SarJ + B0032 - TALEsp1 - WT T7 t</li> | ||
+ | <li>0.5 T7 p - RiboJ + B0032 - Juniper GFP - WT T7 t</li> | ||
+ | <li>T7_sp1 p - RiboJ + B0032 - Juniper GFP - WT T7 t</li> | ||
+ | <li>T7 p - RiboJ + B0032 - Juniper GFP - WT T7 t</li> | ||
+ | <li>0.5 T7_sp1 p - RiboJ + B0032 - Juniper GFP - WT T7 t</li> | ||
+ | <li>Pbhr - SarJ + B0032 - TALEsp1 - WT T7 t</li> | ||
+ | <li>Pbhr - RiboJ + B0032 - Juniper GFP - WT T7 t</li> | ||
+ | <li>0.5 T7 p - SarJ + B0032 - TALEsp1 - WT T7 t</li> | ||
+ | <li>T7 p - SarJ + B0032 - TALEsp1 - WT T7 t</li> | ||
+ | <li>Pbhr - Sarj + B0032 - TALEsp1 - WT T7 t</li> | ||
+ | <li>0.5 T7 p - RiboJ + B0032 - GFP - WT T7 t</li> | ||
+ | <li>0.1 T7 p - RiboJ + B0032 - GFP - WT T7 t</li> | ||
+ | <li>19bp T7 p - RiboJ + B0032 - GFP - WT T7 t</li> | ||
+ | <li>19bp 0.5 T7 p - RiboJ + B0032 - GFP - WT T7 t</li> | ||
+ | <li>19bp 0.1 T7 p - RiboJ + B0032 - GFP - WT T7 t</li> | ||
+ | <li>T7sp_sp1 p - RiboJ + B0032 - GFP - WT T7 t</li> | ||
+ | <li>Pbhr_sp1 - RiboJ + Universal RBS - GFP - WT T7 t</li> | ||
+ | <li>T7 p - SarJ + Universal RBS - GFP - WT T7 t</li> | ||
+ | |||
+ | </ul> | ||
+ | |||
+ | After assembling the level 1's, the following level M's were assembled: | ||
+ | <ul> | ||
+ | <li>T7p TALEsp1 - Dummy 2 - Dummy 3 - T7p TALEsp1</li> | ||
+ | <li>T7p TALEsp1 - Dummy 2 - Dummy 3 - T7p GFP</li> | ||
+ | <li>0.5 T7p TALEsp1 - Dummy 2 - Dummy 3 - 0.5 T7_sp1 p GFP</li> | ||
+ | <li>0.5 T7p TALEsp1 - Dummy 2 - Dummy 3 - 0.5 T7p GFP</li> | ||
+ | <li>0.5 T7p TALEsp1 - Dummy 2 - Dummy 3 - T7_sp1 p GFP</li> | ||
+ | <li>T7p TALEsp1 - Dummy 2 - Dummy 3 - 0.5 T7_sp1 p GFP</li> | ||
+ | <li>T7p TALEsp1 - T7p TALEsp1</li> | ||
+ | <li>T7p TALEsp1 - T7p GFP</li> | ||
+ | <li>0.5 T7p TALEsp1 - 0.5 T7_sp1 p GFP</li> | ||
+ | <li>T7p Universal RBS TALEsp1 - T7_sp1 p Universal RBS GFP</li> | ||
+ | <li>Pbhr Universal RBS TALEsp1 - Pbhr_sp1 Universal RBS GFP</li> | ||
+ | <li>Pbhr Universal RBS TALEsp1 - Pbhr_sp1 v2 Universal RBS GFP</li> | ||
+ | <li>Pbhr Universal RBS TALEsp1 - Pbhr Universal RBS GFP</li> | ||
+ | <li>Pbhr SarJ B00032 TALEsp1 - Pbhr Universal GFP</li> | ||
+ | </ul> | ||
+ | |||
+ | </td></tr> | ||
+ | <tr> | ||
+ | <td>Results: | ||
+ | </td> | ||
+ | <td>To be added</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="modal-footer"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <td colspan="3"></td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="month">September</td> | ||
+ | |||
+ | <td rowspan=2 class="modalTD"> | ||
+ | <!-- Trigger/Open The Modal --> | ||
+ | <button class="block modal-button" href="#myModal12">Copy Number Independence</button> | ||
+ | |||
+ | <!-- The Modal --> | ||
+ | <div id="myModal12" class="modal"> | ||
+ | |||
+ | <!-- Modal content --> | ||
+ | <div class="modal-content"> | ||
+ | <div class="modal-header"> | ||
+ | <span class="close">×</span> | ||
+ | <h2>Copy Number Independence</h2> | ||
+ | </div> | ||
+ | <div class="modal-body"> | ||
+ | <p>Some text in the Modal Body</p> | ||
+ | <p>Some other text...</p> | ||
+ | </div> | ||
+ | <div class="modal-footer"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </td> | ||
+ | <td rowspan=2 class="modalTD"> | ||
+ | <!-- Trigger/Open The Modal --> | ||
+ | <button class="block modal-button" href="#myModal13">Transcriptional Variance Independence</button> | ||
+ | |||
+ | <!-- The Modal --> | ||
+ | <div id="myModal13" class="modal"> | ||
+ | |||
+ | <!-- Modal content --> | ||
+ | <div class="modal-content"> | ||
+ | <div class="modal-header"> | ||
+ | <span class="close">×</span> | ||
+ | <h2>Transcriptional Variance Independence</h2> | ||
+ | </div> | ||
+ | <div class="modal-body"> | ||
+ | <p>Some text in the Modal Body</p> | ||
+ | <p>Some other text...</p> | ||
+ | </div> | ||
+ | <div class="modal-footer"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </td> | ||
+ | <td rowspan=2 class="modalTD"> | ||
+ | <!-- Trigger/Open The Modal --> | ||
+ | <button class="block modal-button" href="#myModal14">Translation Variance Independence</button> | ||
+ | |||
+ | <!-- The Modal --> | ||
+ | <div id="myModal14" class="modal"> | ||
+ | |||
+ | <!-- Modal content --> | ||
+ | <div class="modal-content"> | ||
+ | <div class="modal-header"> | ||
+ | <span class="close">×</span> | ||
+ | <h2>Translation Variance Independence</h2> | ||
+ | </div> | ||
+ | <div class="modal-body"> | ||
+ | <p>Some text in the Modal Body</p> | ||
+ | <p>Some other text...</p> | ||
+ | </div> | ||
+ | <div class="modal-footer"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="month">October</td> | ||
+ | |||
+ | </tr> | ||
+ | </table> | ||
</div> | </div> | ||
Revision as of 13:55, 27 September 2019
![Design](https://static.igem.org/mediawiki/2019/e/e4/T--TUDelft--Notebook_logo.png)
Orthogonal Replication
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Predictable and Transferable Expression
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