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− | <p> In our system we exploit a commonly applied control system known as an Incoherent Feed Forward Loop (iFFL), in which an activator regulates both a gene and a repressor of the gene ... . This control system is established through the expression of a Transcription activator-like effector (TALE) protein. TALE proteins recognize DNA by a simple DNA-binding mechanism which can be altered to recognize any sequence you want … . In our system the TALE protein binds to the promoter of a gene of interest and thus respresses the expression of it. .... has previously described this system and showed how it results in independence of copy number for a gene of interest | + | <p> I In our system we exploit a commonly applied control system known as an Incoherent Feed Forward Loop (iFFL), in which an activator regulates both a gene and a repressor of the gene ... . This control system is established through the expression of a Transcription activator-like effector (TALE) protein. TALE proteins recognize DNA by a simple DNA-binding mechanism which can be altered to recognize any sequence you want … . In our system the TALE protein binds to the promoter of a gene of interest and thus respresses the expression of it. .... has previously described this system and showed how it results in independence of copy number for a gene of interest. |
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Revision as of 16:53, 4 September 2019
![Protocol Experiments](https://static.igem.org/mediawiki/2019/0/03/T--TUDelft--Modeling_logo.png)
Overview - Kinetic Model
I In our system we exploit a commonly applied control system known as an Incoherent Feed Forward Loop (iFFL), in which an activator regulates both a gene and a repressor of the gene ... . This control system is established through the expression of a Transcription activator-like effector (TALE) protein. TALE proteins recognize DNA by a simple DNA-binding mechanism which can be altered to recognize any sequence you want … . In our system the TALE protein binds to the promoter of a gene of interest and thus respresses the expression of it. .... has previously described this system and showed how it results in independence of copy number for a gene of interest.