Difference between revisions of "Team:TUDelft/Notebook"

Line 570: Line 570:
 
                     <td>The following constructs were expressed in vitro and run on a protein gel to determine whether there was amplification of the gene:
 
                     <td>The following constructs were expressed in vitro and run on a protein gel to determine whether there was amplification of the gene:
 
                       <ul>
 
                       <ul>
−
                        <li>0.1 T7 promoter - U RBS - DNAP - WT T7 terminator</li>
+
                    <li><a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918034">DNAP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>0.5 T7 promoter - U RBS - DNAP - WT T7 terminator</li>
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918034">DNAP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>WT T7 promoter - U RBS - DNAP - WT T7 terminator</li>
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918034">DNAP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>0.1 T7 promoter - U RBS - TP - WT T7 terminator</li>
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918001">TP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>0.5 T7 promoter - U RBS - TP - WT T7 terminator</li>
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918001">TP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>WT T7 promoter - U RBS - TP - WT T7 terminator</li>
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918001">TP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>0.1 T7 promoter - U RBS - p5 - WT T7 terminator</li>
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918002">p5</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>0.5 T7 promoter - U RBS - p5 - WT T7 terminator</li>  
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918002">p5</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>WT T7 promoter - U RBS - p5 - WT T7 terminator</li>
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918002">p5</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>0.1 T7 promoter - U RBS - p6 - WT T7 terminator</li>
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918003">p6</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>0.5 T7 promoter - U RBS - p6 - WT T7 terminator</li>
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918003">p6</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>WT T7 promoter - U RBS - p6 - WT T7 terminator</li></ul>
+
                                <li><a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918014">Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918003">p6</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
 +
                       
 
                       <br>
 
                       <br>
 
                       Subsequently, a fraction of the sample was analyzed with Mass Spectrometry.
 
                       Subsequently, a fraction of the sample was analyzed with Mass Spectrometry.
Line 996: Line 997:
 
                       Here a quick reminder of what the constructs were:
 
                       Here a quick reminder of what the constructs were:
 
                       <ul>
 
                       <ul>
−
                        <li>L1_A: Promoter<sub>BHR</sub>sp1- RiboJ- Uni RBS - Juniper GFP- T7 terminator</li>
+
                      <li>L1_A: <a href="http://parts.igem.org/Part:BBa_K2918011">PromoterBHRsp1</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_J97005">Juniper GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>L1_B: T7_sp1 promoter - RiboJ-UniRBS - Harmonized GFP - T7 terminator</li>
+
                                <li>L1_B: <a href="http://parts.igem.org/Part:BBa_K2918010">T7sp1 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918037">Harmonized GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>L1_C: Promoter<sub>BHR</sub> - Sarj-UniRBS - TALEsp1 - T7 terminator variance</li>
+
                                <li>L1_C: <a href="http://parts.igem.org/Part:BBa_K2918000">Pbhr</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a> -<a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li>
−
                        <li>L1_D: T7 promoter - SarJ-UniRBS- TALEsp1 - T7 terminator variance </li>
+
                                <li>L1_D: <a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a> - <a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li>
−
                        <li>L1_E: T7 promoter - RiboJ-UniRBS - Harmonized GFP -T7 terminator</li>
+
                                <li>L1_E: <a href="http://parts.igem.org/Part:BBa_K2918007">WT T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918037">Harmonized GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>L1_F: 0.5 T7 promoter - SarJ-UniRBS- TALEsp1 - T7 terminator variance</li>
+
                                <li>L1_F: <a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a> - <a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li>
−
                        <li>L1_G: 0.1 T7 promoter - SarJ-UniRBS - TALEsp1- T7 terminator</li>
+
                                <li>L1_G: <a href="http://parts.igem.org/Part:BBa_K2918005">Weak T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918036">SarJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918008">TALEsp1</a>- <a href="http://parts.igem.org/Part:BBa_K2918015">WT T7 terminator</a></li>
−
                        <li>L1_H: 0.5 T7 promoter - RiboJ-UniRBS- Harmonized GFP - T7 terminator</li>
+
                                <li>L1_H: <a href="http://parts.igem.org/Part:BBa_K2918006">Medium T7 promoter</a> - <a href="http://parts.igem.org/Part:BBa_K2918038">RiboJ-Universal RBS</a> - <a href="http://parts.igem.org/Part:BBa_K2918037">Harmonized GFP</a> - <a href="http://parts.igem.org/Part:BBa_K2918016">WT T7 terminator variant</a></li>
 
                       </ul>
 
                       </ul>
 
                       <br>
 
                       <br>

Revision as of 22:24, 21 October 2019

Sci-Phi 29

Orthogonal Replication


Cloning Testing of Plasmid
April
May
June
July
August
September
October


Controllable Expression


Cloning Testing of Plasmid
April
May
June
July
August
September
October