Difference between revisions of "Team:TUDelft"

 
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         <style>
 
         <style>
  
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            .containerhome {
 
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                width: 100%;
 
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                height: 100%;
 
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                margin: 0 auto 40px;
 
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            .cardhome {
 
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                width: 100%;
 
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                height: 100%;
 
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            .cardhome div {
 
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                height: 100%;
 
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                width: 100%;
 
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                color: black;
 
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                text-align: center;
 
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                font-weight: bold;
 
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            .cardhome .front {
 
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                background: black;
 
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                justify-content: center;
 
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                align-items: center;
 
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            .cardhome .front p {
 
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                margin:auto;
 
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            .cardhome .back p {
 
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                margin: auto;
 
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            .cardhome .back {
 
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                -webkit-transform: rotateY( 180deg);
 
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                -moz-transform: rotateY( 180deg);
 
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                -o-transform: rotateY( 180deg);
 
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                transform: rotateY( 180deg);
 
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                display: flex;
 
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                justify-content: center;
 
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                align-items: center;
 
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            }
 
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            .homepage {
 
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                height:100%;
 
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                width:100%;
 
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             body{
 
             body{
 
                 background-color:#ffffff}
 
                 background-color:#ffffff}
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            .as_hero{position:relative;background-size:cover;background-repeat:no-repeat;background-attachment:fixed;background-position:top;height:100vh}
 
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            video{min-width:100%;max-width:none!important;min-height:100%;position:absolute;top:50%;left:50%;transform:translateX(-50%) translateY(-50%)}
 
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            .video-container{height:100%;width:100%;overflow:hidden;position:relative}
 
  
  
 
             .Banner{
 
             .Banner{
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                background: linear-gradient( rgba(255, 255, 255,1), rgba(0, 0, 0,1));
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              background: linear-gradient( rgba(188, 233, 246,1), rgba(251, 251, 251,1));
 
                 background-repeat: no-repeat;
 
                 background-repeat: no-repeat;
 
                 background-size:100% 100%;  
 
                 background-size:100% 100%;  
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             }
 
             }
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             .hero-text {
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             .hero-home {
 
                 text-align: center;
 
                 text-align: center;
 
                 position: absolute;
 
                 position: absolute;
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                 border-style: solid;
 
                 border-style: solid;
 
                 border-width:medium;
 
                 border-width:medium;
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                 color: #0072b2 !important;
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                 color: #0072b2!important;
 
                 padding: 1%;
 
                 padding: 1%;
 
                 text-align: center;
 
                 text-align: center;
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             }
 
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             .buttontu2 {
 
             .buttontu2 {
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                border-color: #0072b2;
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                border-style: solid;
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                border-width:medium;
 +
                color: #0072b2;!important;
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                padding: 0.5%;
 +
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 +
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                 border-color: #ffffff;
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             }
 
             }
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             .buttontu2:hover {
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             .buttontu3:hover {
 
                 background-color: #ffffff;
 
                 background-color: #ffffff;
 
                 opacity: 0.75;  
 
                 opacity: 0.75;  
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                 color:#000000!important;
 
                 color:#000000!important;
 
             }
 
             }
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             .centerjustify2 {
 
             .centerjustify2 {
 
                 margin-left: auto;
 
                 margin-left: auto;
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                 max-width: 100%;
 
                 max-width: 100%;
 
                 position: relative;
 
                 position: relative;
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                margin:auto;
 
             }
 
             }
  
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                 height: 20px;
 
                 height: 20px;
 
                 width: 20px;
 
                 width: 20px;
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                 margin: 0 2px;
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                 margin: 0.6%;
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                 border-radius: 50%;
 
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                 transition: background-color 0.6s ease;
 
                 transition: background-color 0.6s ease;
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             width:80%;
 
             width:80%;
 
             }  
 
             }  
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  .containerhome {
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.containerhome {
 
                 width: 100%;
 
                 width: 100%;
 
                 height: auto;
 
                 height: auto;
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             }
 
             }
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.cardhome {
 
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}
 
}
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.cardhome div {
 
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                width: 100%;
 
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}}
 
 
#my_centered_buttons { display: flex; justify-content: center; }
 
#my_centered_buttons { display: flex; justify-content: center; }
  
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.centerfoto {
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  display: block;
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  margin-left: auto;
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  margin-right: auto;
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  margin-top:4%;
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  width: 80%;
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}
 +
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@media screen and (max-width: 800px) {
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.centerfoto {
 +
  display: block;
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  margin-left: auto;
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  margin-right: auto;
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  margin-top:18%;
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  margin-bottom:6%;
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  width: 85%;
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}
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}
 
         </style>
 
         </style>
  
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     <body>
 
     <body>
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        <div class="as_hero">
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        <div class="Banner">
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             <section class="containerhome">
+
 
 +
             <div class="display-2 mb-0">
 +
              <div class="slideshow-container">
 +
<br>
 +
<div class="mySlides fade">
 +
  <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/c/c8/T--TUDelft--HomeAfter.png" alt="slide 1" >
  
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                <div class="cardhome">
 
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                    <div class="front">
 
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                        <img src="https://static.igem.org/mediawiki/2019/a/a9/T--TUDelft--homepagefront.jpeg" alt="bacteria1" class="homepage">
 
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                    </div>
 
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                    <div class="back id="my_centered_buttons">
 
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<div class="hero-home">
 
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                        <img src="https://static.igem.org/mediawiki/2019/6/68/T--TUDelft--achterkant.jpg" alt="bacteria2" class="homepage">
 
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<a href="#ScrollDown1" class="buttontu2 jump"><p style="font-size:xx-large; "> <b> <i class='fas fa-angle-double-down'></i></b></p></a>
 
 
</div>
 
</div>
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                    </div>
 
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                </div>
 
  
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            </section>
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<div class="mySlides fade">
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  <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/5/5b/T--TUDelft--HomeAfter5.png" alt="slide 2" >
  
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</div>
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<div class="mySlides fade">
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  <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/3/3b/T--TUDelft--HomeAfter8.png" alt="slide 3" >
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<div class="mySlides fade">
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  <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/2/2e/T--TUDelft--HomeAfter4.png" alt="slide 4" >
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  <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/e/e4/T--TUDelft--HomeAfter3.png" alt="slide 5" >
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<div class="mySlides fade">
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  <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/9/95/T--TUDelft--HomeAfter7.png" alt="slide 6" >
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<div class="mySlides fade">
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  <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/8/85/T--TUDelft--HomeAfter6.png" alt="slide 7" >
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  <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/e/e3/T--TUDelft--HomeAfter2.png" alt="slide 8" >
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</div>
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<div style="text-align:center">
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  <span class="dot" onclick="currentSlide(1)"></span>
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            </div>           
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        </div>
  
 
         <div style="background-color:#fbfbfb;">
 
         <div style="background-color:#fbfbfb;">
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                 </div>
 
                 </div>
  
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                <br>
 
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                <br>
 
 
                 <br>
 
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                 <div class="centerjustify2" style="text-align:center;">
 
                 <div class="centerjustify2" style="text-align:center;">
 
                     <br>
 
                     <br>
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                     <p style="font-size:xx-large;"> <b> Engineering organism specific parts and utilizing existing parts across different species is extremely laborious and expensive </b> </p>
+
                     <p style="font-size:xx-large;"> <b> Engineering organism-specific parts and utilizing existing parts across different species is extremely laborious and expensive </b> </p>
 
                         <p style="font-size:large;">which restricts the scope of synthetic biology to a small subset of the bacterial cosmos.</p>
 
                         <p style="font-size:large;">which restricts the scope of synthetic biology to a small subset of the bacterial cosmos.</p>
 
                     <br>  
 
                     <br>  
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             </div>
 
             </div>
 
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        <br>
 
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                 <div class="left" style="text-align:center;">
 
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                         <p>hm</p>
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                         <p style="color:rgba(0,0,0,0);">.</p>
 
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                         <p>hm</p>
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                         <p style="color:rgba(0,0,0,0);">.</p>
  
 
                     </div>  
 
                     </div>  
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                     <div class="right">
 
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                         <div class="centerjustify2">
 
                         <div class="centerjustify2">
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                             <p style="font-size:large; line-height: 200%;"> In our project, we developed the <b style="font-size:xx-large;color:#0072b2;">Sci-Phi 29 platform</b> that aims to expand the repertoire of bacterial species and broaden the range of substrates and environmental conditions which is currently applied in synthetic biology.</p>
+
                             <p style="font-size:large; line-height: 200%;"> We developed <b style="font-size:xx-large;color:#0072b2;">Sci-Phi 29</b>, a platform that expands the scope of synthetic biology by providing essential tools required to engineer a repertoire of bacterial species.</p>
 
                             <br>
 
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                             <br>
 
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                 <p><b style="font-size:xx-large;color:#0072b2;">Sci-Phi 29</b></p>  
 
                 <p><b style="font-size:xx-large;color:#0072b2;">Sci-Phi 29</b></p>  
  
−
                 <p style="width:60%;margin-left:auto; margin-right:auto;font-size:large;">is a standardized and user-friendly platform which allows expressing of your gene of interest in a controllable manner across different bacterial species and independently of the host.</p><br>
+
                 <p style="width:60%;margin-left:auto; margin-right:auto;font-size:x-large;"> A user-friendly platform that allows engineering your bacteria of choice through:</p><br>
 
             </div>
 
             </div>
 
         </div>
 
         </div>
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                         <center style="font-size:large;"><b>Find more about how we applied this unique system in our project!</b></center>
 
                         <center style="font-size:large;"><b>Find more about how we applied this unique system in our project!</b></center>
 
                         <br>
 
                         <br>
−
                         <center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Design" class="buttontu "><b>Read More</b></a></center>                       
+
                         <center><a href="https://2019.igem.org/Team:TUDelft/Design#orthorep" class="buttontu "><b>Read More</b></a></center>                       
 
                     </div>  
 
                     </div>  
 
                 </div>   
 
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                     <div class="centerjustify2">
 
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                         <center><img src = "https://static.igem.org/mediawiki/2019/5/56/T--TUDelft--logocontrol.png" alt="Controllability" style="width:49.7%;"></center>
 
                         <center><img src = "https://static.igem.org/mediawiki/2019/5/56/T--TUDelft--logocontrol.png" alt="Controllability" style="width:49.7%;"></center>
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                         <p style="font-size:large; line-height: 200%;"> To tackle the issue of <b style="font-size:x-large;color:#0072b2;">variation in expression</b> across species, we took our platform to the next level by integrating the concept of controllability, which is based on a <b style="font-size:x-large;color:#0072b2;">systems engineering</b> approach. </p>
+
                         <p style="font-size:large; line-height: 200%;"> To tackle the issue of <b style="font-size:x-large;color:#0072b2;">variation in expression</b> across bacterial species, we applied <b style="font-size:x-large;color:#0072b2;">control systems</b> thinking to achieve controllability. </p>
 
                         <br>
 
                         <br>
 
                         <p style="font-size:large; line-height: 200%;">We included the following variables in our <a href="https://2019.igem.org/Team:TUDelft/Model"><u style="font-size:x-large;color:#0072b2;">model</u></a> and <a href="https://2019.igem.org/Team:TUDelft/Results"><u style="font-size:x-large;color:#0072b2;">experiments</u></a>:</p>
 
                         <p style="font-size:large; line-height: 200%;">We included the following variables in our <a href="https://2019.igem.org/Team:TUDelft/Model"><u style="font-size:x-large;color:#0072b2;">model</u></a> and <a href="https://2019.igem.org/Team:TUDelft/Results"><u style="font-size:x-large;color:#0072b2;">experiments</u></a>:</p>
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                                 <a href="https://2019.igem.org/Team:TUDelft/Model"> <img src = "https://static.igem.org/mediawiki/2019/3/33/T--TUDelft--logoplasmidcopynumber.png" alt="Logo Plasmid Copy Number" style="width:100%;"></a>
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                                 <a href="https://2019.igem.org/Team:TUDelft/Model#TranslationalVariations"><img src = "https://static.igem.org/mediawiki/2019/b/b4/T--TUDelft--logo_transcription.png" alt="Logo Translational Variations" style="width:100%;"></a>
 
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                         <br><br> <center style="font-size:large;"><b>Do you want to learn more about the details?</b></center>
 
                         <br><br> <center style="font-size:large;"><b>Do you want to learn more about the details?</b></center>
 
                         <br>
 
                         <br>
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                         <center><a href="https://2019.igem.org/Team:TUDelft/Design" class="buttontu"><b>Click here</b></a></center>  
+
                         <center><a href="https://2019.igem.org/Team:TUDelft/Design#control" class="buttontu"><b>Click here</b></a></center>  
  
 
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                     </div>         
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                     <br><br> <center style="font-size:x-large;"><i>Are you curious about the impact of our Sci-Phi 29 platform?</i></center>
 
                     <br><br> <center style="font-size:x-large;"><i>Are you curious about the impact of our Sci-Phi 29 platform?</i></center>
 
                     <br>
 
                     <br>
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                     <center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Human_Practices" class="buttontu2"><b>Click Here</b></a></center>
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                     <center><a href="https://2019.igem.org/Team:TUDelft/Human_Practices" class="buttontu2"><b>Click Here</b></a></center>
 
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                     <br>
 
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<p style=" font-size:xx-large;color:#0072b2;"> <b>Explore the microbial world with us! </b> </p>
 
<p style=" font-size:xx-large;color:#0072b2;"> <b>Explore the microbial world with us! </b> </p>
 
                         <br>
 
                         <br>
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                         <p style="font-size:large;"> We as a team are <b style="font-size:x-large;color:#0072b2;">fascinated by this microbial diversity</b> and wanted to share our fascination with the rest of the world. That is why our goal this year was to introduce the general public, from children to your neighbor to your teacher, to the hidden world of microbes. We organized <b style="font-size:x-large;color:#0072b2;">multiple events</b> because we wanted to make sure that everyone has <b style="font-size:x-large;color:#0072b2;">access</b> to the invisible microbial world.</p>
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                         <p style="font-size:large;"> We as a team are <b style="font-size:x-large;color:#0072b2;">fascinated by this microbial diversity</b> and wanted to share our fascination with the rest of the world. That is why our goal was to introduce the hidden world of microbes to the general public, from children to your neighbor to your teacher. We organized <b style="font-size:x-large;color:#0072b2;">multiple events</b> because we wanted to make sure that everyone has <b style="font-size:x-large;color:#0072b2;">access</b> to the invisible microbial world.</p>
 
<br>
 
<br>
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                         <center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Public_Engagement" class="buttontu2"><b>Read More</b></a></center>
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                         <center><a href="https://2019.igem.org/Team:TUDelft/Public_Engagement" class="buttontu2"><b>Read More</b></a></center>
 
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Latest revision as of 04:43, 14 December 2019

Sci-Phi 29


Project logo
Results logo
Parts logo
Model logo
Human Pratices logo



Engineering organism-specific parts and utilizing existing parts across different species is extremely laborious and expensive

which restricts the scope of synthetic biology to a small subset of the bacterial cosmos.


Parts road




.


Sci-Phi 29

.

We developed Sci-Phi 29, a platform that expands the scope of synthetic biology by providing essential tools required to engineer a repertoire of bacterial species.



Read More


How does Sci-Phi 29 work?



Different Bacteria
Arrow
Different Bacteria

Sci-Phi 29

A user-friendly platform that allows engineering your bacteria of choice through:


Arrow split

Orthogonality

To express genetic tools across multiple bacterial species, we were inspired by the replication machinery of the phi29 bacteriophage. Its unique protein-primed based replication greatly simplifies the design of replication systems. This system is able to replicate a linear piece of DNA by using only four proteins.



Orthogonal Replication System


Find more about how we applied this unique system in our project!

Read More
Controllability

To tackle the issue of variation in expression across bacterial species, we applied control systems thinking to achieve controllability.


We included the following variables in our model and experiments:



Logo Plasmid Copy Number
Logo Transcriptional Variations
Logo Translational Variations


Do you want to learn more about the details?

Click here


Sci-Phi 29 applications

To envision a future where our Sci-Phi 29 platform can be used to tackle a real world problem, we created a hypothetical use-case scenario. We theoretically applied Sci-Phi 29 to engineer P. putida to be able to convert microplastics from waste water streams.



Are you curious about the impact of our Sci-Phi 29 platform?

Click Here


Explore the microbial world with us!


We as a team are fascinated by this microbial diversity and wanted to share our fascination with the rest of the world. That is why our goal was to introduce the hidden world of microbes to the general public, from children to your neighbor to your teacher. We organized multiple events because we wanted to make sure that everyone has access to the invisible microbial world.


Read More
Fold Scope Event




Find out more about other aspects of our project

Project logo
Results logo
Parts logo

Click here to meet our team!


Team Picture