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</style> | </style> | ||
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− | + | <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/c/c8/T--TUDelft--HomeAfter.png" alt="slide 1" > | |
− | + | </div> | |
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+ | <div class="mySlides fade"> | ||
+ | <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/5/5b/T--TUDelft--HomeAfter5.png" alt="slide 2" > | ||
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+ | </div> | ||
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+ | <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/e/e4/T--TUDelft--HomeAfter3.png" alt="slide 5" > | ||
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+ | </div> | ||
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+ | <div class="mySlides fade"> | ||
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+ | <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/9/95/T--TUDelft--HomeAfter7.png" alt="slide 6" > | ||
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+ | <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/8/85/T--TUDelft--HomeAfter6.png" alt="slide 7" > | ||
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+ | <img class="centerfoto" src = "https://static.igem.org/mediawiki/2019/e/e3/T--TUDelft--HomeAfter2.png" alt="slide 8" > | ||
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+ | </div> | ||
+ | </div> | ||
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+ | <div style="text-align:center"> | ||
+ | <span class="dot" onclick="currentSlide(1)"></span> | ||
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<div style="background-color:#fbfbfb;"> | <div style="background-color:#fbfbfb;"> | ||
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<div class="centerjustify2"> | <div class="centerjustify2"> | ||
+ | <div id="ScrollDown1"></div> | ||
<div class="rowhm"> | <div class="rowhm"> | ||
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<div class="centerjustify2" style="text-align:center;"> | <div class="centerjustify2" style="text-align:center;"> | ||
<br> | <br> | ||
− | <p style="font-size:xx-large;"> <b> Engineering organism specific parts and utilizing existing parts across different species is extremely laborious and expensive </b> </p> | + | <p style="font-size:xx-large;"> <b> Engineering organism-specific parts and utilizing existing parts across different species is extremely laborious and expensive </b> </p> |
<p style="font-size:large;">which restricts the scope of synthetic biology to a small subset of the bacterial cosmos.</p> | <p style="font-size:large;">which restricts the scope of synthetic biology to a small subset of the bacterial cosmos.</p> | ||
<br> | <br> | ||
− | <img class=" | + | <img class="banneroh" src = "https://static.igem.org/mediawiki/2019/8/84/T--TUDelft--steps_parts.png" alt="Parts road"> |
</div> | </div> | ||
<br> | <br> | ||
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<div class="right"> | <div class="right"> | ||
<div class="centerjustify2"> | <div class="centerjustify2"> | ||
− | <p style="font-size:large; line-height: 200%;"> | + | <p style="font-size:large; line-height: 200%;"> We developed <b style="font-size:xx-large;color:#0072b2;">Sci-Phi 29</b>, a platform that expands the scope of synthetic biology by providing essential tools required to engineer a repertoire of bacterial species.</p> |
<br> | <br> | ||
<br> | <br> | ||
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<div style="text-align:center; background-color:#fbfbfb;" > | <div style="text-align:center; background-color:#fbfbfb;" > | ||
<br> | <br> | ||
− | <img src = "https://static.igem.org/mediawiki/2019/1/1c/T--TUDelft--Bacteria_home.png" alt="Different Bacteria" | + | <img class="banneroh40" src = "https://static.igem.org/mediawiki/2019/1/1c/T--TUDelft--Bacteria_home.png" alt="Different Bacteria" > |
</div> | </div> | ||
</div> | </div> | ||
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<div style="text-align:center; background-color:#fbfbfb;" > | <div style="text-align:center; background-color:#fbfbfb;" > | ||
− | <img src = "https://static.igem.org/mediawiki/2019/d/de/T--TUDelft--ArrowPattern.png" alt="Arrow" | + | <img class="banneroh10" src = "https://static.igem.org/mediawiki/2019/d/de/T--TUDelft--ArrowPattern.png" alt="Arrow"> |
</div> | </div> | ||
</div> | </div> | ||
<div data-aos="fade-up" data-aos-duration="3000"> | <div data-aos="fade-up" data-aos-duration="3000"> | ||
− | <div style="text-align:center; background-color:#fbfbfb;cursor: pointer;" onclick="window.location='https://2019.igem.org/Team:TUDelft/ | + | <div style="text-align:center; background-color:#fbfbfb;cursor: pointer;" onclick="window.location='https://2019.igem.org/Team:TUDelft/Design';"> |
− | <img src = "https://static.igem.org/mediawiki/2019/d/d3/T--TUDelft--Sci-PhiKit.png" alt="Different Bacteria" | + | <img class="banneroh18" src = "https://static.igem.org/mediawiki/2019/d/d3/T--TUDelft--Sci-PhiKit.png" alt="Different Bacteria"> |
<p><b style="font-size:xx-large;color:#0072b2;">Sci-Phi 29</b></p> | <p><b style="font-size:xx-large;color:#0072b2;">Sci-Phi 29</b></p> | ||
− | <p style="width:60%;margin-left:auto; margin-right:auto;font-size:large;"> | + | <p style="width:60%;margin-left:auto; margin-right:auto;font-size:x-large;"> A user-friendly platform that allows engineering your bacteria of choice through:</p><br> |
</div> | </div> | ||
</div> | </div> | ||
<div data-aos="fade-up" data-aos-duration="3000"> | <div data-aos="fade-up" data-aos-duration="3000"> | ||
<div style="text-align:center; background-color:#fbfbfb;" > | <div style="text-align:center; background-color:#fbfbfb;" > | ||
− | <img src = "https://static.igem.org/mediawiki/2019/9/9e/T--TUDelft--Arrowpatternsplit.png" alt="Arrow split" | + | <img class="banneroh" src = "https://static.igem.org/mediawiki/2019/9/9e/T--TUDelft--Arrowpatternsplit.png" alt="Arrow split"> |
<br> | <br> | ||
<br> | <br> | ||
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<br> | <br> | ||
<center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Design" class="buttontu "><b>Read More</b></a></center> | <center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Design" class="buttontu "><b>Read More</b></a></center> | ||
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<div class="centerjustify2"> | <div class="centerjustify2"> | ||
<center><img src = "https://static.igem.org/mediawiki/2019/5/56/T--TUDelft--logocontrol.png" alt="Controllability" style="width:49.7%;"></center> | <center><img src = "https://static.igem.org/mediawiki/2019/5/56/T--TUDelft--logocontrol.png" alt="Controllability" style="width:49.7%;"></center> | ||
− | <p style="font-size:large; line-height: 200%;"> To tackle the issue of <b style="font-size:x-large;color:#0072b2;">variation in expression</b> across species, we | + | <p style="font-size:large; line-height: 200%;"> To tackle the issue of <b style="font-size:x-large;color:#0072b2;">variation in expression</b> across bacterial species, we applied <b style="font-size:x-large;color:#0072b2;">control systems</b> thinking to achieve controllability. </p> |
<br> | <br> | ||
<p style="font-size:large; line-height: 200%;">We included the following variables in our <a href="https://2019.igem.org/Team:TUDelft/Model"><u style="font-size:x-large;color:#0072b2;">model</u></a> and <a href="https://2019.igem.org/Team:TUDelft/Results"><u style="font-size:x-large;color:#0072b2;">experiments</u></a>:</p> | <p style="font-size:large; line-height: 200%;">We included the following variables in our <a href="https://2019.igem.org/Team:TUDelft/Model"><u style="font-size:x-large;color:#0072b2;">model</u></a> and <a href="https://2019.igem.org/Team:TUDelft/Results"><u style="font-size:x-large;color:#0072b2;">experiments</u></a>:</p> | ||
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<div class="boxx3 columnhm" > | <div class="boxx3 columnhm" > | ||
− | <img src = "https://static.igem.org/mediawiki/2019/3/33/T--TUDelft--logoplasmidcopynumber.png" alt="Logo Plasmid Copy Number" style="width:100%;"> | + | <a href="https://2019.igem.org/Team:TUDelft/Model#PlasmidCopyNumber"> <img src = "https://static.igem.org/mediawiki/2019/3/33/T--TUDelft--logoplasmidcopynumber.png" alt="Logo Plasmid Copy Number" style="width:100%;"></a> |
</div> | </div> | ||
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<div class="boxx3 columnhm" > | <div class="boxx3 columnhm" > | ||
− | <img src = "https://static.igem.org/mediawiki/2019/6/6f/T--TUDelft--logo_transcription2.png" alt="Logo Transcriptional Variations" style="width:100%;"> | + | <a href="https://2019.igem.org/Team:TUDelft/Model#TranscriptionalVariations"><img src = "https://static.igem.org/mediawiki/2019/6/6f/T--TUDelft--logo_transcription2.png" alt="Logo Transcriptional Variations" style="width:100%;"></a> |
</div> | </div> | ||
<div class="boxx3 columnhm"> | <div class="boxx3 columnhm"> | ||
− | <img src = "https://static.igem.org/mediawiki/2019/b/b4/T--TUDelft--logo_transcription.png" alt="Logo Translational Variations" style="width:100%;"> | + | <a href="https://2019.igem.org/Team:TUDelft/Model#TranslationalVariations"><img src = "https://static.igem.org/mediawiki/2019/b/b4/T--TUDelft--logo_transcription.png" alt="Logo Translational Variations" style="width:100%;"></a> |
</div> | </div> | ||
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<br> | <br> | ||
<br> | <br> | ||
− | <img src = "https://static.igem.org/mediawiki/2019/2/21/T--TUDelft--sci-phitogether.png" alt="Sci-Phi 29 applications" style=" | + | <img class="banneroh18" src = "https://static.igem.org/mediawiki/2019/2/21/T--TUDelft--sci-phitogether.png" alt="Sci-Phi 29 applications" style="align:center;"> |
<p style="font-size:large;">To envision a future where our Sci-Phi 29 platform can be used to tackle a real world problem, we created a <b style="font-size:x-large;color:#0072b2;">hypothetical use-case scenario</b>. We theoretically applied Sci-Phi 29 to engineer <b style="font-size:x-large;color:#0072b2;"><i>P. putida</i></b> to be able to convert <b style="font-size:x-large;color:#0072b2;">microplastics</b> from waste water streams.</p> | <p style="font-size:large;">To envision a future where our Sci-Phi 29 platform can be used to tackle a real world problem, we created a <b style="font-size:x-large;color:#0072b2;">hypothetical use-case scenario</b>. We theoretically applied Sci-Phi 29 to engineer <b style="font-size:x-large;color:#0072b2;"><i>P. putida</i></b> to be able to convert <b style="font-size:x-large;color:#0072b2;">microplastics</b> from waste water streams.</p> | ||
− | <br><br> <center style="font-size:x-large;"><i>Are you | + | <br><br> <center style="font-size:x-large;"><i>Are you curious about the impact of our Sci-Phi 29 platform?</i></center> |
<br> | <br> | ||
<center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Human_Practices" class="buttontu2"><b>Click Here</b></a></center> | <center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Human_Practices" class="buttontu2"><b>Click Here</b></a></center> | ||
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<div data-aos="fade-up" data-aos-duration="3000"> | <div data-aos="fade-up" data-aos-duration="3000"> | ||
− | <div style="background-color:# | + | <div style="background-color:#e1e1e1;" > |
<br> | <br> | ||
<div class="centerjustify2" style="text-align:center;"> | <div class="centerjustify2" style="text-align:center;"> | ||
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<p style=" font-size:xx-large;color:#0072b2;"> <b>Explore the microbial world with us! </b> </p> | <p style=" font-size:xx-large;color:#0072b2;"> <b>Explore the microbial world with us! </b> </p> | ||
<br> | <br> | ||
− | <p style="font-size:large;"> We as a team are <b style="font-size:x-large;color:#0072b2;">fascinated by this microbial diversity</b> and wanted to share our fascination with the rest of the world. That is why our goal this year was to introduce the general public, from children to your neighbor to your teacher, to the hidden world of microbes. We organized <b style="font-size:x-large;color:#0072b2;">multiple events</b> because we wanted to make sure that everyone has <b style="font-size:x-large;color:#0072b2;"> | + | <p style="font-size:large;"> We as a team are <b style="font-size:x-large;color:#0072b2;">fascinated by this microbial diversity</b> and wanted to share our fascination with the rest of the world. That is why our goal this year was to introduce the general public, from children to your neighbor to your teacher, to the hidden world of microbes. We organized <b style="font-size:x-large;color:#0072b2;">multiple events</b> because we wanted to make sure that everyone has <b style="font-size:x-large;color:#0072b2;">access</b> to the invisible microbial world.</p> |
− | + | <br> | |
<center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Public_Engagement" class="buttontu2"><b>Read More</b></a></center> | <center><a target="_blank" href="https://2019.igem.org/Team:TUDelft/Public_Engagement" class="buttontu2"><b>Read More</b></a></center> | ||
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<br> | <br> | ||
<br> | <br> | ||
− | <p style=" font-size:xx-large;text-align:center;"> <b>Find more about | + | <p style=" font-size:xx-large;text-align:center;"> <b>Find out more about other aspects of our project</b> </p> |
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<div class="rowhm"> | <div class="rowhm"> | ||
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</div></a> | </div></a> | ||
− | <a href="https://2019.igem.org/Team:TUDelft/ | + | <a href="https://2019.igem.org/Team:TUDelft/Safety"><div class="boxx columnhm"> |
<img src = "https://static.igem.org/mediawiki/2019/8/8e/T--TUDelft--Safety_mini_logo.png" alt="Results logo" style="width:100%;"> | <img src = "https://static.igem.org/mediawiki/2019/8/8e/T--TUDelft--Safety_mini_logo.png" alt="Results logo" style="width:100%;"> | ||
</div></a> | </div></a> | ||
<a href="https://2019.igem.org/Team:TUDelft/Collaborations"><div class="boxx columnhm"> | <a href="https://2019.igem.org/Team:TUDelft/Collaborations"><div class="boxx columnhm"> | ||
− | <img src = "https://static.igem.org/mediawiki/2019/ | + | <img src = "https://static.igem.org/mediawiki/2019/b/bd/T--TUDelft--Collaborations_mini_logo.png" alt="Parts logo" style="width:100%;"> |
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+ | <p style="font-size:xx-large;color:#0072b2;text-align: center;"><a href="https://2019.igem.org/Team:TUDelft/Team" ><b>Click here</b></a> to meet our team!</p> | ||
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Latest revision as of 06:41, 6 December 2019
Engineering organism-specific parts and utilizing existing parts across different species is extremely laborious and expensive
which restricts the scope of synthetic biology to a small subset of the bacterial cosmos.
![Parts road](https://static.igem.org/mediawiki/2019/8/84/T--TUDelft--steps_parts.png)
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![Sci-Phi 29](https://static.igem.org/mediawiki/2019/e/e7/T--TUDELFT--logonavbar-white.png)
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We developed Sci-Phi 29, a platform that expands the scope of synthetic biology by providing essential tools required to engineer a repertoire of bacterial species.
Read More
How does Sci-Phi 29 work?
![Different Bacteria](https://static.igem.org/mediawiki/2019/1/1c/T--TUDelft--Bacteria_home.png)
![Arrow](https://static.igem.org/mediawiki/2019/d/de/T--TUDelft--ArrowPattern.png)
![Different Bacteria](https://static.igem.org/mediawiki/2019/d/d3/T--TUDelft--Sci-PhiKit.png)
Sci-Phi 29
A user-friendly platform that allows engineering your bacteria of choice through:
![Arrow split](https://static.igem.org/mediawiki/2019/9/9e/T--TUDelft--Arrowpatternsplit.png)
![Orthogonality](https://static.igem.org/mediawiki/2019/f/f0/T--TUDelft--logorepli.png)
To express genetic tools across multiple bacterial species, we were inspired by the replication machinery of the phi29 bacteriophage. Its unique protein-primed based replication greatly simplifies the design of replication systems. This system is able to replicate a linear piece of DNA by using only four proteins.
![Orthogonal Replication System](https://static.igem.org/mediawiki/2019/2/2d/T--TUDelft--orthoreplihome.png)
![Controllability](https://static.igem.org/mediawiki/2019/5/56/T--TUDelft--logocontrol.png)
To tackle the issue of variation in expression across bacterial species, we applied control systems thinking to achieve controllability.
We included the following variables in our model and experiments:
![Sci-Phi 29 applications](https://static.igem.org/mediawiki/2019/2/21/T--TUDelft--sci-phitogether.png)
To envision a future where our Sci-Phi 29 platform can be used to tackle a real world problem, we created a hypothetical use-case scenario. We theoretically applied Sci-Phi 29 to engineer P. putida to be able to convert microplastics from waste water streams.
Explore the microbial world with us!
We as a team are fascinated by this microbial diversity and wanted to share our fascination with the rest of the world. That is why our goal this year was to introduce the general public, from children to your neighbor to your teacher, to the hidden world of microbes. We organized multiple events because we wanted to make sure that everyone has access to the invisible microbial world.
Click here to meet our team!
![Team Picture](https://static.igem.org/mediawiki/2019/2/23/T--TUDelft--Team2.png)