Difference between revisions of "Team:SMMU-China/Parts"

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      href="https://2019.igem.org/wiki/index.php?title=Template:SMMU-China/CSS_main&action=raw&ctype=text/css"/>
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<!--<link rel="stylesheet" href="main.css" />-->
  
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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. The iGEM provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<div class="highlight decoration_background">
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<h3>Note</h3>
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<p>Note that parts must be well documented on each part's <a href="http://parts.igem.org/Main_Page">Main Page on the Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
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This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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</div>
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</div>
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<div class="clear extra_space"></div>
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    <div class="big_framework">
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<div class="line_divider"></div>
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        <div class="index-nav">
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<div class="clear extra_space"></div>
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            <div class="index-nav-frame clearfix">
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                <div class="nav-line">
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                    <div class="index-nav-frame-logo-mobile">
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                            <img src="https://static.igem.org/mediawiki/2019/0/0f/T--SMMU-China--wukong_logo.svg" alt="Wukong">
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                        </div>
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                </div>
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                <div class="nav-small" tabindex="-1">
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                    <div class="nav-small-focus" tabindex="-1">
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                    </div>
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                    <img src="https://static.igem.org/mediawiki/2019/7/70/T--SMMU-China--header_icon.png"/>
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                </div>
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                <div class="index-nav-frame-logo">
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                    <img src="https://static.igem.org/mediawiki/2019/0/0f/T--SMMU-China--wukong_logo.svg" alt="Wukong">
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                </div>
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                <div class="index-nav-frame-line first-one" tabindex="-1">
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                    <a href="https://2019.igem.org/Team:SMMU-China">Home</a>
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                    <div class="index-nav-frame-line-center need-to-be-hidden">
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China">Home</a>
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                        </div>
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                    </div>
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                    <div class="index-nav-frame-line-focus" tabindex="-1"></div>
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                </div>
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                <div class="index-nav-frame-line" tabindex="-1">
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                    PROJECT
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                    <div class="index-nav-frame-line-center">
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Description">Description</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Design">Design</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Model">Model</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Software">Software</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Notebook">Notebook</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Demonstrate">Demonstrate</a>
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                        </div>
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                    </div>
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                    <div class="index-nav-frame-line-focus" tabindex="-1"></div>
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                </div>
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                <div class="index-nav-frame-line active" tabindex="-1">
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                    PARTS
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                    <div class="index-nav-frame-line-center">
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Parts">Parts Overview</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Basic_Part">Basic Parts</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Composite_Part">Composite Parts</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Part_Collection">Part Collection</a>
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                        </div>
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                    </div>
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                    <div class="index-nav-frame-line-focus" tabindex="-1"></div>
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                </div>
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                <div class="index-nav-frame-line" tabindex="-1">
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                    <a href="https://2019.igem.org/Team:SMMU-China/Safety">Safety</a>
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                    <div class="index-nav-frame-line-center need-to-be-hidden">
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Safety">Safety</a>
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                        </div>
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                    </div>
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                    <div class="index-nav-frame-line-focus" tabindex="-1"></div>
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                </div>
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                <div class="index-nav-frame-line HP-button" tabindex="-1">
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                    HUMAN PRACTICES
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                    <div class="index-nav-frame-line-center">
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Human_Practices">HP overview</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Human_Practices#to_ihp">Integrated HP</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Public_Engagement">Education & Engagement</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Collaborations">Collaborations</a>
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                        </div>
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                    </div>
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                    <div class="index-nav-frame-line-focus" tabindex="-1"></div>
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                </div>
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                <div class="index-nav-frame-line" tabindex="-1">
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                    Team
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                    <div class="index-nav-frame-line-center">
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Team">Team</a>
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                        </div>
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                        <div class="index-nav-frame-line-li">
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                            <a href="https://2019.igem.org/Team:SMMU-China/Attributions">Attributions</a>
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                        </div>
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                    </div>
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                    <div class="index-nav-frame-line-focus" tabindex="-1"></div>
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                </div>
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            </div>
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        </div>
  
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    <div class="blank_framework"></div>
  
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    <div class="page_banner">
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        <img src="https://static.igem.org/mediawiki/2019/2/24/T--SMMU-China--banner_parts.jpg"
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            alt="page_banner">
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    </div>
  
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    <div class="page_big_title_box">
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        <div class="page_big_title">Parts Overview</div>
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        <div class="page_big_subtitle">The detail of parts.</div>
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    </div>
  
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    <div class="medium_framework">
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        <br><br><h2 style="text-align: left">In 2019, SMMU-China has submitted 29 parts. We divide our parts into 3 groups, basic parts, component parts
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            and an improvement part. We also established two parts collections respectively collected synthetic
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            receptors and synthetic transcription factor (SynTF) - synthetic promoter (SynPro) pairs we have
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            constructed.</h2>
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    </div>
  
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<div class="column two_thirds_size">
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    <div class="medium_framework">
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<div class="highlight decoration_B_full">
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        <h1>Basic Parts</h1>
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        <p>We have constructed a cellular cascade device consists of many basic parts. First, we set several receptors.
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            In the core device, we used synNotch and CAR as our synthetic receptors, while GEMS and MESA was used in the
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            peripherals. All of the receptors consist of several structural domains, with each of them has their own
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            characteristics. Second, we chose some synthetic transcription factors to activate expression of target
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            genes. Moreover, there were soluble molecules to connect the two devices.</p>
 +
        <div align="center">
 +
            <table class="smmu-table">
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                <thead>
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                <tr>
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                    <th>No.</th>
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                    <th>Biobrick Name</th>
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                    <th>Description</th>
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                </tr>
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                </thead>
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                <tbody>
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                <tr>
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                    <td data-label="No.">BBa_K3132000<br>
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                        BBa_K3132001<br>
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                        BBa_K3132002<br>
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                        BBa_K3132003
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                    </td>
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                    <td data-label="Biobrick Name">Gal4-VP64<br>
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                        ZF43-8-VP64<br>
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                        ZF21-16-VP64<br>
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                        PIP-VP64
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                    </td>
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                    <td data-label="Description">Synthetic transcription factors fused to intracellular domain of synNotch.</td>
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                </tr>
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                <tr>
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                    <td data-label="No.">BBa_K3132004</td>
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                    <td data-label="Biobrick Name">MinimalCMV</td>
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                    <td data-label="Description">Fused to promoters to enhance priming activity.</td>
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                </tr>
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                <tr>
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                    <td data-label="No.">BBa_K3132005<br>
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                        BBa_K3132006
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                    </td>
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                    <td data-label="Biobrick Name">anti-HER2 scFV<br>
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                        NL domains through cytoplasmic
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                    </td>
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                    <td data-label="Description">The extracellular and transmembrane domains of synNotch.</td>
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                </tr>
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                <tr>
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                    <td data-label="No.">BBa_K3132020</td>
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                    <td data-label="Biobrick Name">SynNotch-anti-HER2 </td>
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                    <td data-label="Description">Characterization of synNotch</td>
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                </tr>
 +
                <tr>
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                    <td data-label="No.">BBa_K3132007<br>
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                        BBa_K3132008<br>
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                        BBa_K3132009
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                    </td>
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                    <td data-label="Biobrick Name">anti-GC4N scFV<br>
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                        anti-IL15 scFV<br>
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                        anti-Flag scFV
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                    </td>
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                    <td data-label="Description">The extracellular domain of GEMS</td>
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                </tr>
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                <tr>
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                    <td data-label="No.">BBa_K3132016</td>
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                    <td data-label="Biobrick Name">Generalized extracellular molecule sensor platform (GEMS)</td>
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                    <td data-label="Description">Scaffold and characterization of GEMS</td>
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                </tr>
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                <tr>
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                    <td data-label="No.">BBa_K3132012<br>
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                        BBa_K3132013
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                    </td>
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                    <td data-label="Biobrick Name">PIP-Elk1 transcription factor<br>
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                        PIP-Elk1 promoter
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                    </td>
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                    <td data-label="Description">Transcription factor (SynTF) - synthetic promoter (SynPro) pair for MAPK pathway activated by GEMS</td>
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                </tr>
 +
                <tr>
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                    <td data-label="No.">BBa_K3132018<br>
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                        BBa_K3132019
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                    </td>
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                    <td data-label="Biobrick Name">8×suntag<br>
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                        8×Flag
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                    </td>
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                    <td data-label="Description">Tag proteins used as soluble signal molecules</td>
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                </tr>
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                <tr>
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                    <td data-label="No.">BBa_K3132021<br>
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                        BBa_K3132022
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                    </td>
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                    <td data-label="Biobrick Name">Cetuximab scFv-CAR<br>
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                        trastuzumab4D5-5 scFv-CAR
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                    </td>
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                    <td data-label="Description">Two structure domains of CAR</td>
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                </tr>
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                </tbody>
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            </table>
 +
        </div>
  
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<h3>Adding parts to the registry</h3>
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    </div>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Documentation includes the characterization data of your parts.</p>
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    <div class="medium_framework">
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<div class="button_link">
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        <h1>Component parts</h1>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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        <p>To enhance the activity of promoters, we fused a MinimalCMV to each promoters, and constructed four types of
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ADD PARTS
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            component parts.</p>
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</a>
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        <div align="center">
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</div>
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            <table class="smmu-table">
 +
                <thead>
 +
                <tr>
 +
                    <th>No.</th>
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                    <th>Biobrick Name</th>
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                    <th>Description</th>
 +
                </tr>
 +
                </thead>
 +
                <tbody>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132100<br>
 +
                        BBa_K3132101
 +
                    </td>
 +
                    <td data-label="Biobrick Name">UAS_MinimalCMV Promotor<br>
 +
                        PIP_MinimalCMV Promotor
 +
                    </td>
 +
                    <td data-label="Description">Activate expression of CARs and soluble </td>
 +
                </tr>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132102<br>
 +
                        BBa_K3132103
 +
                    </td>
 +
                    <td data-label="Biobrick Name">ZF21-16_MinimalCMV Promotor<br>
 +
                        ZF43-8_MinimalCMV Promotor
 +
                    </td>
 +
                    <td data-label="Description">Molecules in the core device</td>
 +
                </tr>
 +
                </tbody>
 +
            </table>
 +
        </div>
 +
    </div>
  
−
</div>
+
    <div class="medium_framework">
−
</div>
+
        <h1>Improvement parts</h1>
 +
        <p>Based on the transcription factor PIP-KRAB (BBa_K2446045) which has inhibitory function that have been
 +
            submitted by Fudan 2017 and a new TF Gal4-VP64 (BBa_K3132000) which has activation function, we fused both
 +
            of them and got a bifunctional transcription factor that is able to both inhibit and activate the expression
 +
            of target genes.</p>
 +
        <div align="center">
 +
            <table class="smmu-table">
 +
                <thead>
 +
                <tr>
 +
                    <th>No.</th>
 +
                    <th>Biobrick Name</th>
 +
                    <th>Description</th>
 +
                </tr>
 +
                </thead>
 +
                <tbody>
 +
                <tr>
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                    <td data-label="No.">BBa_K3132105</td>
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                    <td data-label="Biobrick Name">PIP-KRAB-Gal4-VP64</td>
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                    <td data-label="Description">Bifunctional transcription factor</td>
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        <h1>Parts collection</h1>
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        <p>We collected four types of synthetic receptors and four pairs of synTF-synPro. More information on
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            <a href="https://2019.igem.org/Team:SMMU-China/Part_Collection"
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              style="text-decoration: underline; color: #3b52b6; font-weight: bold">Parts collection.</a></p><br><br>
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            <div class="footer_title">Contact Us</div>
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                E-Mail: igem-smmu@smmu.edu.cn<br>
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                Phone: +86 021-81870925
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                © 2019. SMMU. All rights reserved.
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<h3>Inspiration</h3>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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<h3>What information do I need to start putting my parts on the Registry?</h3>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<li>Creator</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Design considerations</li>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
 
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
 
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Latest revision as of 23:04, 21 October 2019

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Parts Overview
The detail of parts.


In 2019, SMMU-China has submitted 29 parts. We divide our parts into 3 groups, basic parts, component parts and an improvement part. We also established two parts collections respectively collected synthetic receptors and synthetic transcription factor (SynTF) - synthetic promoter (SynPro) pairs we have constructed.

Basic Parts

We have constructed a cellular cascade device consists of many basic parts. First, we set several receptors. In the core device, we used synNotch and CAR as our synthetic receptors, while GEMS and MESA was used in the peripherals. All of the receptors consist of several structural domains, with each of them has their own characteristics. Second, we chose some synthetic transcription factors to activate expression of target genes. Moreover, there were soluble molecules to connect the two devices.

No. Biobrick Name Description
BBa_K3132000
BBa_K3132001
BBa_K3132002
BBa_K3132003 		Gal4-VP64
ZF43-8-VP64
ZF21-16-VP64
PIP-VP64 		Synthetic transcription factors fused to intracellular domain of synNotch.
BBa_K3132004 MinimalCMV Fused to promoters to enhance priming activity.
BBa_K3132005
BBa_K3132006 		anti-HER2 scFV
NL domains through cytoplasmic 		The extracellular and transmembrane domains of synNotch.
BBa_K3132020 SynNotch-anti-HER2 Characterization of synNotch
BBa_K3132007
BBa_K3132008
BBa_K3132009 		anti-GC4N scFV
anti-IL15 scFV
anti-Flag scFV 		The extracellular domain of GEMS
BBa_K3132016 Generalized extracellular molecule sensor platform (GEMS) Scaffold and characterization of GEMS
BBa_K3132012
BBa_K3132013 		PIP-Elk1 transcription factor
PIP-Elk1 promoter 		Transcription factor (SynTF) - synthetic promoter (SynPro) pair for MAPK pathway activated by GEMS
BBa_K3132018
BBa_K3132019 		8×suntag
8×Flag 		Tag proteins used as soluble signal molecules
BBa_K3132021
BBa_K3132022 		Cetuximab scFv-CAR
trastuzumab4D5-5 scFv-CAR 		Two structure domains of CAR

Component parts

To enhance the activity of promoters, we fused a MinimalCMV to each promoters, and constructed four types of component parts.

No. Biobrick Name Description
BBa_K3132100
BBa_K3132101 		UAS_MinimalCMV Promotor
PIP_MinimalCMV Promotor 		Activate expression of CARs and soluble
BBa_K3132102
BBa_K3132103 		ZF21-16_MinimalCMV Promotor
ZF43-8_MinimalCMV Promotor 		Molecules in the core device

Improvement parts

Based on the transcription factor PIP-KRAB (BBa_K2446045) which has inhibitory function that have been submitted by Fudan 2017 and a new TF Gal4-VP64 (BBa_K3132000) which has activation function, we fused both of them and got a bifunctional transcription factor that is able to both inhibit and activate the expression of target genes.

No. Biobrick Name Description
BBa_K3132105 PIP-KRAB-Gal4-VP64 Bifunctional transcription factor

Parts collection

We collected four types of synthetic receptors and four pairs of synTF-synPro. More information on Parts collection.



Contact Us
E-Mail: igem-smmu@smmu.edu.cn
Phone: +86 021-81870925
© 2019. SMMU. All rights reserved.