Team:SMMU-China/Parts
In 2019, SMMU-China has submitted 29 parts. We divide our parts into 3 groups, basic parts, component parts and an improvement part. We also established two parts collections respectively collected synthetic receptors and synthetic transcription factor (SynTF) - synthetic promoter (SynPro) pairs we have constructed.
Basic Parts
We have constructed a cellular cascade device consists of many basic parts. First, we set several receptors. In the core device, we used synNotch and CAR as our synthetic receptors, while GEMS and MESA was used in the peripherals. All of the receptors consist of several structural domains, with each of them has their own characteristics. Second, we chose some synthetic transcription factors to activate expression of target genes. Moreover, there were soluble molecules to connect the two devices.
| No. | Biobrick Name | Description |
|---|---|---|
| BBa_K3132000 BBa_K3132001 BBa_K3132002 BBa_K3132003 |
Gal4-VP64 ZF43-8-VP64 ZF21-16-VP64 PIP-VP64 |
Synthetic transcription factors fused to intracellular domain of synNotch. |
| BBa_K3132004 | MinimalCMV | Fused to promoters to enhance priming activity. |
| BBa_K3132005 BBa_K3132006 |
anti-HER2 scFV NL domains through cytoplasmic |
The extracellular and transmembrane domains of synNotch. |
| BBa_K3132020 | SynNotch-anti-HER2 | Characterization of synNotch |
| BBa_K3132007 BBa_K3132008 BBa_K3132009 |
anti-GC4N scFV anti-IL15 scFV anti-Flag scFV |
The extracellular domain of GEMS |
| BBa_K3132016 | Generalized extracellular molecule sensor platform (GEMS) | Scaffold and characterization of GEMS |
| BBa_K3132012 BBa_K3132013 |
PIP-Elk1 transcription factor PIP-Elk1 promoter |
Transcription factor (SynTF) - synthetic promoter (SynPro) pair for MAPK pathway activated by GEMS |
| BBa_K3132018 BBa_K3132019 |
8×suntag 8×Flag |
Tag proteins used as soluble signal molecules |
| BBa_K3132021 BBa_K3132022 |
Cetuximab scFv-CAR trastuzumab4D5-5 scFv-CAR |
Two structure domains of CAR |
Component parts
To enhance the activity of promoters, we fused a MinimalCMV to each promoters, and constructed four types of component parts.
| No. | Biobrick Name | Description |
|---|---|---|
| BBa_K3132100 BBa_K3132101 |
UAS_MinimalCMV Promotor PIP_MinimalCMV Promotor |
Activate expression of CARs and soluble |
| BBa_K3132102 BBa_K3132103 |
ZF21-16_MinimalCMV Promotor ZF43-8_MinimalCMV Promotor |
Molecules in the core device |
Improvement parts
Based on the transcription factor PIP-KRAB (BBa_K2446045) which has inhibitory function that have been submitted by Fudan 2017 and a new TF Gal4-VP64 (BBa_K3132000) which has activation function, we fused both of them and got a bifunctional transcription factor that is able to both inhibit and activate the expression of target genes.
| No. | Biobrick Name | Description |
|---|---|---|
| BBa_K3132105 | PIP-KRAB-Gal4-VP64 | Bifunctional transcription factor |
Parts collection
We collected four types of synthetic receptors and four pairs of synTF-synPro. More information on Parts collection.