In 2019, SMMU-China has submitted 29 parts. We divide our parts into 3 groups, basic parts, component parts and an improvement part. We also established two parts collections respectively collected synthetic receptors and synthetic transcription factor (SynTF) - synthetic promoter (SynPro) pairs we have constructed.
We have constructed a cellular cascade device consists of many basic parts. First, we set several receptors. In the core device, we used synNotch and CAR as our synthetic receptors, while GEMS and MESA was used in the peripherals. All of the receptors consist of several structural domains, with each of them has their own characteristics. Second, we chose some synthetic transcription factors to activate expression of target genes. Moreover, there were soluble molecules to connect the two devices.
|Synthetic transcription factors fused to intracellular domain of synNotch.|
|BBa_K3132004||MinimalCMV||Fused to promoters to enhance priming activity.|
NL domains through cytoplasmic
|The extracellular and transmembrane domains of synNotch.|
|BBa_K3132020||SynNotch-anti-HER2||Characterization of synNotch|
|The extracellular domain of GEMS|
|BBa_K3132016||Generalized extracellular molecule sensor platform (GEMS)||Scaffold and characterization of GEMS|
|PIP-Elk1 transcription factor
|Transcription factor (SynTF) - synthetic promoter (SynPro) pair for MAPK pathway activated by GEMS|
|Tag proteins used as soluble signal molecules|
|Two structure domains of CAR|
To enhance the activity of promoters, we fused a MinimalCMV to each promoters, and constructed four types of component parts.
|Activate expression of CARs and soluble|
|Molecules in the core device|
Based on the transcription factor PIP-KRAB (BBa_K2446045) which has inhibitory function that have been submitted by Fudan 2017 and a new TF Gal4-VP64 (BBa_K3132000) which has activation function, we fused both of them and got a bifunctional transcription factor that is able to both inhibit and activate the expression of target genes.
|BBa_K3132105||PIP-KRAB-Gal4-VP64||Bifunctional transcription factor|
We collected four types of synthetic receptors and four pairs of synTF-synPro. More information on Parts collection.