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Revision as of 15:37, 21 October 2019


Protocols

General Protocols

Agarose Gel Electrophoresis
SDS-Page Protein electrophoresis
Transformation

DNA Extraction

Microneedle Patches Fabrication
DNA extraction

RPA Amplification

RPA from banana DNA
RPA Amplification with Agdia AmplifyRP® kit
RPA Amplification with TwistAmp kit

Toeholds

Toehold switch assembly
Toehold testing
Toehold plate reader settings

Signal Generation

2-step PCR
CDO Expression in a cell-free system

Cell-Free Systems

Protein Purification
Ribosome Purification
Buffer preparation
Energy Solution preparation
Protein expression