Difference between revisions of "Team:SMMU-China/Basic Part"
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China">Home</a> | |
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| − | + | PROJECT | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Description">Description</a> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Design">Design</a> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Model">Model</a> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Software">Software</a> | |
| − | + | </div> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Notebook">Notebook</a> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Demonstrate">Demonstrate</a> | |
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| − | + | PARTS | |
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| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Parts">Parts Overview</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Basic_Part">Basic Parts</a> | |
| − | + | </div> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Part_Collection">Part Collection</a> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Safety">Safety</a> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Safety">Safety</a> | |
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| − | + | </div> | |
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| − | + | HUMAN PRACTICES | |
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| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Human_Practices">HP overview</a> | |
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| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Human_Practices#to_ihp">Integrated HP</a> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Public_Engagement">Education & Engagement</a> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Collaborations">Collaborations</a> | |
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| − | + | Team | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Team">Team</a> | |
| − | + | </div> | |
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| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Attributions">Attributions</a> | |
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| − | + | <div class="page_banner"> | |
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| + | </div> | ||
| − | + | <div class="page_big_title_box"> | |
| − | + | <div class="page_big_title">Basic Part</div> | |
| − | + | <div class="page_big_subtitle">The detail of basic part.</div> | |
| − | + | </div> | |
| − | + | <div class="medium_framework"> | |
| − | + | <h1>Our favorite basic part - GEMS</h1> | |
| − | + | <p>This part is a highly generalizable synthetic receptor that has a wild sensing of input signals and effective | |
| − | + | activation of gene expression. As developed in 2015, this receptor still contains much possibility for | |
| + | transformation in synthetic biology. Our team determined to use this receptor, make some transformation as | ||
| + | interest and give it a new mission in our nanobots <a href="https://2019.igem.org/Team:SMMU-China" | ||
| + | style="text-decoration: underline">WuKong</a>.</p> | ||
| + | </div> | ||
| − | + | <div class="medium_framework"> | |
| − | + | <h1>Structure and mechanism of GEMS</h1> | |
| − | + | <p>GEMS is a dimer of two subunits, each was constructed by three domains, an extracellular domain as an | |
| − | + | anti-suntag scFv, a scaffold transmembrane domain derived from EpoR (erythropoietin receptor) and a | |
| + | FGFR1(fibroblast growth factor receptor 1) intracellular domain that induces MAPK pathway <a | ||
| + | style="text-decoration: underline">(Figure 1a)</a>. When | ||
| + | the MAPK-GEMS recognizes the ligand, each receptor subunit will rotate around its own axis for about 100°, | ||
| + | leads to an activation of transcription factor PIP-Elk which then modulate expression of reporter | ||
| + | gene <a style="text-decoration: underline">(Figure 1b)</a>.</p> | ||
| + | <img style="width: 75%;" src="https://static.igem.org/mediawiki/2019/5/53/T--SMMU-China--parts_basicparts_1.png" | ||
| + | alt="smmu_demonstrate_1" style="margin: 10px 0px;"> | ||
| + | <center>Figure 1. the structure and mechanism of MAPK-GEMS receptor. a.Three domains that mainly constructed | ||
| + | GEMS. b.Mechanism of MAPK-GEMS. | ||
| + | </center> | ||
| + | </div> | ||
| − | + | <div class="medium_framework"> | |
| − | + | <h1> See more </h1> | |
| − | + | <p>Integrated information about GEMS posted on <a href="https://2019.igem.org/Team:SMMU-China/Demonstrate" | |
| − | + | style="text-decoration: underline">Demonstrate</a> page, we | |
| + | also put the characterizations of GEMS | ||
| + | on the Registry of Standard Biological Parts website, welcome to check part <a | ||
| + | href="http://parts.igem.org/Part:BBa_K3132016" | ||
| + | style="text-decoration: underline">(BBa_K3132016)</a> on its page.</p> | ||
| + | <p style="text-decoration: underline; color: #b63b4d"><b>Here we listed other basic parts we have constructed below.</b></p> | ||
| + | <table class="smmu-table"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Gal4-VP64</th> | ||
| + | <th>ZF43-8-VP64</th> | ||
| + | <th>ZF21-16-VP64</th> | ||
| + | <th>PIP-VP64</th> | ||
| + | <th>MinimalCMV</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td data-label="Gal4-VP64"><a href="http://parts.igem.org/Part:BBa_K3132000" target="_blank">(BBa_K3132000)</a> | ||
| + | </td> | ||
| + | <td data-label="ZF43-8-VP64"><a href="http://parts.igem.org/Part:BBa_K3132001" target="_blank">(BBa_K3132001)</a> | ||
| + | </td> | ||
| + | <td data-label="ZF21-16-VP64"><a href="http://parts.igem.org/Part:BBa_K3132002" target="_blank">(BBa_K3132002)</a> | ||
| + | </td> | ||
| + | <td data-label="PIP-VP64"><a href="http://parts.igem.org/Part:BBa_K3132003" target="_blank">(BBa_K3132003)</a> | ||
| + | </td> | ||
| + | <td data-label="MinimalCMV"><a href="http://parts.igem.org/Part:BBa_K3132004" target="_blank">(BBa_K3132004)</a> | ||
| + | </td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>anti-GC4N(suntag) scFv</th> | ||
| + | <th>anti-flag scFv</th> | ||
| + | <th>anti-IL-15 scFv</th> | ||
| + | <th>anti-HER2 scFv</th> | ||
| + | <th>NL domains through cytoplasmic</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td data-label="anti-GC4N(suntag) scFv"><a href="http://parts.igem.org/Part:BBa_K3132007" | ||
| + | target="_blank">(BBa_K3132007)</a></td> | ||
| + | <td data-label="anti-flag scFv"><a href="http://parts.igem.org/Part:BBa_K3132009" target="_blank">(BBa_K3132009)</a> | ||
| + | </td> | ||
| + | <td data-label="anti-IL-15 scFv"><a href="http://parts.igem.org/Part:BBa_K3132008" target="_blank">(BBa_K3132008)</a> | ||
| + | </td> | ||
| + | <td data-label="anti-HER2 scFv"><a href="http://parts.igem.org/Part:BBa_K3132005" target="_blank">(BBa_K3132005)</a> | ||
| + | </td> | ||
| + | <td data-label="NL domains through cytoplasmic"><a href="http://parts.igem.org/Part:BBa_K3132006" | ||
| + | target="_blank">(BBa_K3132006)</a></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>STAT3 transcription factor</th> | ||
| + | <th>pSTAT promoter</th> | ||
| + | <th>PIP-Elk1 transcription factor</th> | ||
| + | <th>PIP-Elk1 promoter</th> | ||
| + | <th>4×UAS</th> | ||
| + | <th>CAR-IL-2-His tag</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td data-label="STAT3 transcription factor"><a href="http://parts.igem.org/Part:BBa_K3132010" | ||
| + | target="_blank">(BBa_K3132010)</a></td> | ||
| + | <td data-label="pSTAT promoter"><a href="http://parts.igem.org/Part:BBa_K3132011" target="_blank">(BBa_K3132011)</a> | ||
| + | </td> | ||
| + | <td data-label="PIP-Elk1 transcription factor"><a href="http://parts.igem.org/Part:BBa_K3132012" | ||
| + | target="_blank">(BBa_K3132012)</a></td> | ||
| + | <td data-label="PIP-Elk1 promoter"><a href="http://parts.igem.org/Part:BBa_K3132013" target="_blank">(BBa_K3132013)</a> | ||
| + | </td> | ||
| + | <td data-label="4×UAS"><a href="http://parts.igem.org/Part:BBa_K3132014" | ||
| + | target="_blank">(BBa_K3132014)</a></td> | ||
| + | <td data-label="CAR-IL-2-His tag"><a href="http://parts.igem.org/Part:BBa_K3132015" target="_blank">(BBa_K3132015)</a> | ||
| + | </td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>human interleukine 15(IL-15)</th> | ||
| + | <th>8×suntag</th> | ||
| + | <th>8×flag</th> | ||
| + | <th>synNotch-anti-HER2</th> | ||
| + | <th>Cetuximab scFv-CAR</th> | ||
| + | <th>trastuzumab4D5-5 scFv-CAR</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td data-label="human interleukine 15(IL-15)"><a href="http://parts.igem.org/Part:BBa_K3132017" | ||
| + | target="_blank">(BBa_K3132017)</a></td> | ||
| + | <td data-label="8×suntag"><a href="http://parts.igem.org/Part:BBa_K3132018" target="_blank">(BBa_K3132018)</a> | ||
| + | </td> | ||
| + | <td data-label="8×flag"><a href="http://parts.igem.org/Part:BBa_K3132019" | ||
| + | target="_blank">(BBa_K3132019)</a></td> | ||
| + | <td data-label="synNotch-anti-HER2"><a href="http://parts.igem.org/Part:BBa_K3132020" target="_blank">(BBa_K3132020)</a> | ||
| + | </td> | ||
| + | <td data-label="Cetuximab scFv-CAR"><a href="http://parts.igem.org/Part:BBa_K3132021" target="_blank">(BBa_K3132021)</a> | ||
| + | </td> | ||
| + | <td data-label="trastuzumab4D5-5 scFv-CAR"><a href="http://parts.igem.org/Part:BBa_K3132022" | ||
| + | target="_blank">(BBa_K3132022)</a></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
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| − | + | <div class="footer_title">Contact Us</div> | |
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| − | + | E-Mail: igem-smmu@smmu.edu.cn<br> | |
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Latest revision as of 13:07, 21 October 2019
Basic Part
The detail of basic part.
Our favorite basic part - GEMS
This part is a highly generalizable synthetic receptor that has a wild sensing of input signals and effective activation of gene expression. As developed in 2015, this receptor still contains much possibility for transformation in synthetic biology. Our team determined to use this receptor, make some transformation as interest and give it a new mission in our nanobots WuKong.
Structure and mechanism of GEMS
GEMS is a dimer of two subunits, each was constructed by three domains, an extracellular domain as an anti-suntag scFv, a scaffold transmembrane domain derived from EpoR (erythropoietin receptor) and a FGFR1(fibroblast growth factor receptor 1) intracellular domain that induces MAPK pathway (Figure 1a). When the MAPK-GEMS recognizes the ligand, each receptor subunit will rotate around its own axis for about 100°, leads to an activation of transcription factor PIP-Elk which then modulate expression of reporter gene (Figure 1b).
See more
Integrated information about GEMS posted on Demonstrate page, we also put the characterizations of GEMS on the Registry of Standard Biological Parts website, welcome to check part (BBa_K3132016) on its page.
Here we listed other basic parts we have constructed below.
| Gal4-VP64 | ZF43-8-VP64 | ZF21-16-VP64 | PIP-VP64 | MinimalCMV | |
|---|---|---|---|---|---|
| (BBa_K3132000) | (BBa_K3132001) | (BBa_K3132002) | (BBa_K3132003) | (BBa_K3132004) | |
| anti-GC4N(suntag) scFv | anti-flag scFv | anti-IL-15 scFv | anti-HER2 scFv | NL domains through cytoplasmic | |
| (BBa_K3132007) | (BBa_K3132009) | (BBa_K3132008) | (BBa_K3132005) | (BBa_K3132006) | |
| STAT3 transcription factor | pSTAT promoter | PIP-Elk1 transcription factor | PIP-Elk1 promoter | 4×UAS | CAR-IL-2-His tag |
| (BBa_K3132010) | (BBa_K3132011) | (BBa_K3132012) | (BBa_K3132013) | (BBa_K3132014) | (BBa_K3132015) |
| human interleukine 15(IL-15) | 8×suntag | 8×flag | synNotch-anti-HER2 | Cetuximab scFv-CAR | trastuzumab4D5-5 scFv-CAR |
| (BBa_K3132017) | (BBa_K3132018) | (BBa_K3132019) | (BBa_K3132020) | (BBa_K3132021) | (BBa_K3132022) |