References
[under construction]
Difference between revisions of "Team:EPFL/Experiments"
| Line 141: | Line 141: | ||
<div class="demo-item"><a href="https://www.protocols.io/view/toehold-testing-pure-system-protein-production-73ehqje"><img src="https://static.igem.org/mediawiki/2019/a/a6/T--EPFL--Toehold_icon.png""></a> | <div class="demo-item"><a href="https://www.protocols.io/view/toehold-testing-pure-system-protein-production-73ehqje"><img src="https://static.igem.org/mediawiki/2019/a/a6/T--EPFL--Toehold_icon.png""></a> | ||
<h6>Toehold functionality test</h6> | <h6>Toehold functionality test</h6> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="col-md-2"> | ||
| + | <div class="demo-item"><a href="https://www.protocols.io/view/plate-reader-setting-8grhtv6"><img src="https://static.igem.org/mediawiki/2019/a/a6/T--EPFL--Toehold_icon.png""></a> | ||
| + | <h6>Toehold plat reader setting</h6> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 11:20, 19 October 2019
Protocols
General Protocols
2-step PCR
Agarose Gel Electrophoresis
SDS Page Protein electrophoresis
Transformation
DNA Extraction
Microneedle Fabrication
Buffer Extraction
Microneedle Extraction
RPA Amplification
RPA from banana DNA
TO ADD
TO ADD
Toeholds
Toehold switch assembly
Toehold functionality test
Toehold plat reader setting
Signal Generation
sfGFP gene assembly
CDO gene
CDO Expression in a cell-free system
Cell-Free System
Ribosome Purification
Buffer preparation