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• Best Basic Part﹀

  ♥	Name	Description	Designer
  ♥	BBa_K2918000	blablablablablablablablablablablabla	TU Delft 2019

  
  

  Hello

• Best Composite Part﹀

  ♥	Name	Description	Designer
  ♥	BBa_K2918000	blablablablablablablablablablablabla	TU Delft 2019

• Best Part Collection﹀

  Engineering non-model organisms requires discovery of organism-specific parts or re-tuning of parts characterized in model organisms. Our part collection provides modular cloning (MoClo) compatible parts that can be used for predictable expression of genes across different bacterial species.
  
  To overcome the need to identify parts for different bacterial hosts, our collection provides parts of the phi29 replication machinery to establish orthogonal replication. Furthermore, the collection consists of regulatory elements (promoters, RBSs, terminators) that work in a plethora of bacterial species.
  
  To insulate the behavior of genetic circuits from variations associated with change in bacterial context, our collection also offers parts essential to append an incoherent feed forward loop (iFFL) to any genetic circuit. iFFL ensures that the gene of interest expression is impervious to changes in copy number, transcriptional and translational rates.
  
  Our collection acts as a platform to future iGEM teams for effortless engineering of their bacteria of choice.

  
  

  Basic Parts

  ♥	Name	Type	Description	Designer	Length
  	BBa_K2918005	Regulatory	0.1 T7 promoter	TUDelft2019	23
  	BBa_K2918006	Regulatory	0.5 T7 promoter	TUDelft2019	23
  	BBa_K2918007	Regulatory	Wild type T7 promoter	TUDelft2019	23
  	BBa_K2918009	Regulatory	0.5 T7 promoter variant with TALEsp1 binding site	TUDelft 2019	41
  	BBa_K2918035	RBS	BBa_B0032 RBS and RiboJ	TUDelft 2019	94
  	BBa_K2918013	RBS	BBa_B0032 RBS and SarJ	TUDelft 2019	98
  ♥	BBa_K2918034	Regulatory	Broad host range promoter	TUDelft 2019	56
  	BBa_K2918011	Regulatory	Broad host range promoter with TALEsp1 binding site	TUDelft 2019	56
  	BBa_K2918037	Coding	Cross-species harmonized GFP	TUDelft 2019	678
  	BBa_K2918061	Other	RBS (B0032) adapted for modular cloning with Ribozymes	TUDelft 2019	25
  	BBa_K2918038	RBS	RiboJ and universal binding site	TUDelft 2019	85
  	BBa_K2918012	Other	Ribozyme J (Ribo J)	TUDelft 2019	75
  	BBa_K2918036	RBS	SarJ and universal ribosome binding site	TUDelft 2019	89
  	BBa_K2918010	Regulatory	T7 promoter with TALEsp1 binding site	TUDelft 2019	41
  	BBa_K2918016	Terminator	T7 terminator variant	TUDelft 2019	48
  	BBa_K2918008	Coding	Transcription Activator like Effector protein (TALEsp1)	TUDelft2019	2640
  	BBa_K2918014	RBS	Universal RBS	TUDelft2019	10
  	BBa_K2918062	RBS	Universal RBS site adapted for modular cloning with ribozymes	TUDelft 2019	16
  	BBa_K2918015	Terminator	Wild type T7 terminator	TUDelft2019	48
  ♥	BBa_K2918000	Coding	Φ29 DNA polymerase (DNAP/p2)	TUDelft2019	1719
  	BBa_K2918003	Coding	Φ29 Double Strand Binding Protein (DSB/p6)	TUDelft2019	315
  	BBa_K2918002	Coding	Φ29 Single Stranded Binding Protein (SSB/p5)	TUDelft2019	375
  ♥	BBa_K2918001	Coding	Φ29 Terminal Protein (TP/p3)	TUDelft2019	801

  
  

  Composite Parts

  ♥	Name	Type	Description	Designer	Length
  	BBa_K2918017	Composite	0.1 T7 promoter - Universal RBS - Φ29 9 TP - WT T7 terminator	TUDelft2019	890
  	BBa_K2918018	Composite	0.5 T7 promoter - Universal RBS - Φ29 TP - T7 terminator	TUDelft2019	904
  ♥	BBa_K2918019	Composite	WT T7 promoter - Universal RBS - Φ29 TP - T7 terminator	TUDelft2019	904
  	BBa_K2918020	Composite	0.1 T7 promoter - Universal RBS - Φ29 DNAP - T7 terminator	TUDelft2019	1822
  	BBa_K2918021	Composite	0.5 T7 promoter - Universal RBS - Φ29 DNAP - T7 terminator	TUDelft2019	1822
  	BBa_K2918022	Composite	WT T7 promoter - Universal RBS - Φ29 DNAP - WT T7 terminator	TUDelft2019	1899
  	BBa_K2918023	Composite	0.1 T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator	TUDelft2019	519
  	BBa_K2918024	Composite	0.5 T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator	TUDelft2019	519
  	BBa_K2918025	Composite	WT T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator	TUDelft2019	519
  	BBa_K2918026	Composite	0.1 T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator	TUDelft2019	579
  	BBa_K2918027	Composite	0.5 T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator	TUDelft2019	579
  	BBa_K2918028	Composite	WT T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator	TUDelft2019	579
  	BBa_K2918030	Composite	WT T7 promoter - Universal RBS - GFP - T7 terminator	TUDelft2019	811
  	BBa_K2918031	Composite	0.1 T7 promoter - Universal RBS - GFP - T7 terminator	TUDelft2019	811
  	BBa_K2918032	Composite	0.5 T7 promoter - Universal RBS - GFP - T7 terminator	TUDelft2019	811