Team:YAU-China/Collaborations
Collaborations
Good ideas are often the result of a collision of ideas. In order to improve the background of the project, conduct the experiment more smoothly, and express more clearly in the PPT presentation, our YAU-China team has conducted very close contact and cooperated with XJTU-CHINA , NWU-China , SCU-China and JNFLS.
Three University Unions in the northwest of China
After the completion of the first part of our experimental project, we conducted many exchanges and cooperated with XJTU-CHINA and NWU- China.
On July 19, our team went to Xi 'an Jiaotong University to hold an exchange meeting with XJTU-CHINA and NWU-China. During this meeting, our three schools introduced our own respective projects to each other and put forward their views and suggestions. After a heated discussion, everyone had a deeper understanding of their own project. Most notably, in order to overcome the difficulty of obtaining the sequence in a timely manner, our three teams reached an agreement to build a PART library with the iGEM officials annual quota of 30kb parts provided for free synthesis. Currently, there are 26 parts in our part library. In addition, other two teams shared a lot of experience to us, so that we, as a new team, could make our goals and tasks clearer in the follow-up work. Here, I express my gratitude to the two teams for their care and hope that everyone can work together to create a better result in this iGEM.
https://2019.igem.org/Team:NWU-China/Collaborations.
https://2019.igem.org/Team:XJTU-CHINA/Collaborations.
Collaborations with NWU-China and SCU-China
When the experiment of the project was completed, we went to Chengdu together with NWU-China to conducte exchange and cooperate with SCU-China of Sichuan University. We visited their lab and they introduced us to their innovative board game of Human Practices, which inspired us to some extent in the later part of Human Practices. Unfortunately, our activity in this part could not be implemented. But, the ideas they brought to us are very useful. Thank you to them and hope them can learn something from this iGEM.
https://2019.igem.org/Team:SCU-China/Meetup
Collaboration with Tianjin
During the process of participating in CCiC, we got acquainted with Tianjin. They were very interested in Pseudomonas aeruginosa of our project, and proposed that 16 schools work together to make an animation to introduce the bacteria species, and we were responsible for the introduction of Pseudomonas aeruginosa .
https://2019.igem.org/Team:Tianjin/Collaborations
There are countless kinds of bacteria in nature, and each kind of bacteria is different in form, structure and function. Through such an animation, we believe that more people can understand the types of bacteria, and make use of them or prevent their harm, which is of great benefit to the whole society. Thanks to Tianjin, I hope they can have a greater breakthrough in this iGEM .
Collaboration with XJTU-CHINA
(1) We provided pSB1K3 plasmid for XJTU-CHINA. To prevent the loss of regulatory plasmids, they used kanamycin resistance, which is different from the precursor plasmid resistance (chloramphenicol), and found the vector plasmid pSB1K3 through kit search. However, the official kit for them did not arrive yet, so we provided help. Unfortunately, they successfully transformed the vector plasmid.
(2) XJTU-CHINA guided the modeling part of our project. As our team participates in the competition independently for the first time, we don't know anything about modeling at first. XJTU-CHINA provided a lot of materials for learning modeling. In view of the safety mechanism of our experiment, they had the question of toxicity of the engineered bacteria and whether it would be harmful to other bacteria after cleavage. Therefore, we adopted XJTU-CHINA's recommendation and knocked out the T3SS gene cluster. Here, we thank XJTU-CHINA for giving us a lot of views and hope that some of our suggestions can be of help to them. At the same time, I hope both teams can gain something in this iGEM.
Collaboration with NWU-China
(1) NWU-China provided us with pSB1K3 and pSB1C3 plasmids, which provided us with many conveniences for testing and improving the experiments of parts. In addition, NWU-China guided our modeling and proposed that the rate of Pf4 production is the difference between the two enzyme rates, which greatly inspired us.
(2) During several communication meetings, we questioned the application design of the project of NWU-China, and proposed to them the suggestion of adding nutrients to the filter paper of activated bacteria. Here, we thank NWU-China for their help and wish them a great success in this competition.
Collaboration with JNFLS
(1) During the CCiC, we encountered an outstanding high school term, JNFLS. This term wanted to using BFD-M4 and TALB-F178Y two mutant enzymes to convert the formaldehyde into XYLULOSE which can be used in dealing with diseases. This forward-looking attracted our term. After the long time contacting, we found that this method can not only be used in the lab, but can be used in the real life especially in the factories as well. So we help them to construct the hardware that can be used in our rooms after the brainstorm together.
(2) In the Oct, we found that there was a little difficulties to finish the wiki effectively, the leader of JNFLS gave us the direct guidance about how to using the procedure and draw a clear outline for us. During this process, both of us finished our project effectively, and established the profound friendship.