We decided to use Yarrowia lipolytica A101 yeast (GRAS status) and Escherichia coli DH5-Alpha. Both organisms are not dangerous to humans.
We worked in step with rigorous safety protocols for use of biological organisms.
Our project complies with iGEM's safety and security rules and policies.
Safety in Lab
We followed the good hygene practices (e.g disinfection and sterilization, separate garbage bins for contaminated waste that is autoclaved before disposal).
We received safety training (e.g good microbial technique, physical biosecurity, personnel biosecurity, chemicals, fire and electrical safety).
Biosafety level 1 laboratory.
We worked under the sterile laminar chamber using gloves and safety glasses when interacting with living oranisms.
Application safety issues
The genes which was implemented into Yarrowia lipolytica are commonly found in nature and do not encode any toxins or potentially harmful proteins.
No safety, ethical and security risks.
Ensuring that filters will be used correctly - they will also be recycled! :)
