Team:Uppsala Universitet/Parts


Parts Overview

The basic parts used in the project were codon optimised for Pichia pastoris and synthesised by IDT. Modifications of basic parts such as addition of ribosome standby sites was performed by inverse-mutagenesis PCR. Construction of composite parts was achieved by gibson assembly.

All used parts are presented in Table 1 and 2. Descriptions of all parts can be found in the Basic parts or Composite parts. The team chose F2A as the best basic part created in the project. The self-cleaving mechanism, the functionality of which we proved in P. pastoris, can be used in a multitude of future projects to coexpress proteins in one mRNA strand in a eukaryotic expression system.

As its best composite part, the Uppsala Team nominates Expression construct HRP-2A-AAO. In this part functional coexpression of horseradish peroxidase and aryl-alcohol oxidase was shown.

Table 1: Parts used for gold medal criteria

# Name Type Description
1 BBa_K3105111 Basic part RBS with ribosome standby site
2 BBa_K3105666 Composite part AsPink with ribosome standby site upstream of RBS

Table 2: Parts used for main project

# Name Type Description
1 BBa_K3105668 Basic part Horseradish peroxidase
2 BBa_K3105667 Basic part Aryl-alcohol oxidase
3 BBa_K3105684 Composite part Expression construct HRP
4 BBa_K3105685 Composite part Expression construct AAO
5 BBa_K3105681 Composite part Expression construct LiP
6 BBa_K3105683 Composite part Expression construct Glox
7 BBa_K3105682 Composite part Expression construct MnP
8 BBa_K3105669 Basic part F2A
9 BBa_K3105670 Basic part eGFP
10 BBa_K3105671 Composite part HRP-2A-eGFP
11 BBa_K3105672 Composite part HRP-2A-AAO
11 BBa_K3105678 Composite part AAO-2A-eGFP
12 BBa_K3105677 Composite part Expression construct HRP-2A-eGFP
13 BBa_K3105680 Composite part Expression construct AAO-2A-eGFP
14 BBa_K3105679 Composite part Expression construct HRP-2A-AAO

Basic Parts

Description of all basic parts.

To Basic Parts

Composite Parts

Description of all Composite Parts.

To Compostie Parts

Parts Collection

Description of parts collection.

To Part collection