Team:Uppsala Universitet/Basic Part
Basic Part
The basic parts used in the project were codon optimized for Pichia pastoris and synthesised by IDT. Modifications of basic parts such as addition of ribosome standby sites was performed by inverse-mutagenesis PCR.
The team chose F2A as the best basic part created in the project. The self-cleaving mechanism, the functionality of which we proved in P. pastoris, can be used in a multitude of future projects to coexpress proteins in one mRNA strand in a eukaryotic expression system.
| # | Name | Type | Description |
|---|---|---|---|
| 1 | BBa_K3105111 | Basic Part | RBS with ribosome standby site |
| 2 | BBa_K3105668 | Basic Part | HRP codon optimised for P. pastoris |
| 3 | BBa_K3105667 | Basic Part | AAO codon optimised for P. pastoris |
| 4 | BBa_K3105669 | Basic Part | F2A |
| 5 | BBa_K3105670 | Basic Part | eGFP |