Team:Tianjin/Parts

Parts Overview


Our team has created some new parts, mainly including the construction of chromosomal stabilization element group, tags, and some parts used in the construction of single base editor.

Chromosomal stabilization element group:

In order to transform centromeres, the core part of our project, into the genome of Saccharomyces cerevisiae cells, we constructed several fragments for transformation. In order to ensure the validity of transformation, we added tags for screening and corresponding promoters and terminators.


>>Please refer to our result page for more details.

Chromosome transfer (mRFP):

In order to directly observe the results of the chromosome transfer after fusion, we inducted mRFP into Saccharomyces cerevisiae, so that we can take photos of the fusants with fluorescence microscope. As a result, we created the following parts and proved them to work properly.


>>Please refer to our result page for more details.

Single base editor section:

We have created a fusion characterization system using CRISPR-dcas9 technology, which is mainly composed of mutant dCas9 fusion protein, gRNA and protospacer. The fusion protein consists of adenine base editor (ABE) and dcas9 protein, which are linked by a rigid linker, and its expression is controlled by PGK1 promoter and terminator. Through its combination with gRNA, we can see that it performs a certain degree of timing editing on the corresponding protospacer. This Biobrick can be used not only for the fusion characterization of these two cells, but also for the recording of specific events in certain cells after iGEM.


>>Please refer to our result page for more details.

Parts Table

Basic Parts

Name Type Description
BBa_K3112000 DNA S.C cenXV
BBa_K3112001 DNA Y.L cenE
BBa_K3112002 Coding dCas9
BBa_K3112004 Regulatory TEF promoter
BBa_K3112005 Terminator CYC1 Terminator
BBa_K3112007 RNA gRNA
BBa_K3112008 DNA Protospacer+PAM
BBa_K3112009 Coding GFP
BBa_K3112010 Coding mRFP use in yeast
BBa_K3112011 Regulatory PGK1 Promoter
BBa_K3112012 Terminator PGK1 Terminator
BBa_K3112013 Coding yEGFP
BBa_K3112014 Regulatory His3 Promoter
BBa_K3112015 Coding His3 Gene
BBa_K3112016 Regulatory LEU2 Promoter
BBa_K3112017 Coding LEU2 Gene
BBa_K3112018 Regulatory URA3 Promoter
BBa_K3112019 Coding URA3 Gene
BBa_K3112020 Coding Hygromycin Resistance Gene(hyg or hph)
BBa_K3112021 Coding A to G adenine Base Editor
BBa_K3112022 Terminator TEF Terminator
BBa_K3112023 Coding APOBEC1 cytidine deaminase
BBa_K3112024 Regulatory GAP Promoter
BBa_K3112025 Terminator ADH1 Terminator
BBa_K3112026 DNA Homologous arm in Yarrowia lipolytica E Chromosome 1
BBa_K3112027 DNA Homologous arm in Yarrowia lipolytica E Chromosome 2
BBa_K3112028 DNA Homologous arm in Saccharomyces cerevisiae V Chromosome 1
BBa_K3112029 DNA Homologous arm in Saccharomyces cerevisiae V Chromosome 2
BBa_K3112030 DNA Homologous arm in Saccharomyces cerevisiae V Chromosome 3
BBa_K3112031 DNA Homologous arm in Saccharomyces cerevisiae V Chromosome 4
BBa_K3112032 DNA Left Homologous Arm for Homologous Recombination of Saccharomyces cerevisiae
BBa_K3112033 DNA Right Homologous Arm for Homologous Recombination of Saccharomyces cerevisiae
BBa_K3112034 DNA Left Homologous Arm for Homologous Recombination of Yarrowia lipolytica
BBa_K3112035 DNA Right Homologous Arm for Homologous Recombination of Yarrowia lipolytica
BBa_K3112037 Coding KanMX

Composite Parts

Name Type Description
BBa_K3112040 Composite Chromosome stabilizing elements example 1
BBa_K3112041 Composite Chromosome stabilizing elements example 2
BBa_K3112042 Composite Chromosome stabilizing elements example 3
BBa_K3112043 Composite mRFP+HIS3 Chromosome Transfer Elements
BBa_K3112044 Composite yEGFP+URA3 Chromosome Transfer Elements
BBa_K3112045 Composite PGK1P+A to G adenine Base Editor+PGK1T
BBa_K3112046 Composite Chromosomal stabilization element group example 3

Improved Parts

Name Type Description
BBa_K3112050 Reporter mRFP reporter with weak constitutive promoter(J23115)

Necessary Parts

Bronze Standard

Name Type Description
BBa_E0422 Reporter ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
BBa_E0430 Reporter EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
BBa_J23110 Regulatory constitutive promoter family member

Silver Standard

Name Type Description
BBa_K3112041 Composite Chromosome stabilizing elements example 2
BBa_K3112042 Composite Chromosome stabilizing elements example 3
BBa_K3112043 Composite mRFP+HIS3 Chromosome Transfer Elements

Gold Standard

Name Type Description
BBa_K3112050 Reporter mRFP reporter with weak constitutive promoter(J23115)