Team:Tianjin/Basic Part

Basic Parts



Saccharomyces cerevisiae is very popular because of its high homologous recombination ability. Many scientists have used it as a tool to assemble small fragments in vivo. At present, by preparing some single fragments with homologous arms and transferring them into Saccharomyces cerevisiae at one time, we can use the homologous recombination mechanism of Saccharomyces cerevisiae to synthesize several genes into a long gene cycle in vivo. This homologous recombination mechanism has enabled many substances, such as purple bacillin, naringin, beta-carotene and lycopene to be expressed in Saccharomyces cerevisiae. However, Saccharomyces cerevisiae is not the most suitable place to produce certain substances, such as some hydrophobic products.

As an oil yeast, Yarrowia lipolytica has a very high lipid content. In recent years, it has been modified as chassis cells to synthesize various lipid products, especially fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids, fatty acid esters, fatty alcohols, sterols, terpenoids and other products. In 2007, the European Union put forward the Lipoyeasts project, aiming at developing Yarrowia lipolytica into a multi-functional fermentation platform. There are two important tasks: first, to construct a metabolic precursor Library of Yarrowia lipolytica to produce value-added products from lipids; second, to transform lipid intermediates into these products by introducing the function of heterologous enzymes isolated from marine hydrocarbon fragmentation bacteria into Yarrowia lipolytica. As a novel strain, Yarrowia lipolytica is far less capable of homologous recombination than Saccharomyces cerevisiae. It will be very difficult to reconstruct yeast in laboratory. At this time, the complementary relationship between Saccharomyces cerevisiae and Yersinia lipolytica arises spontaneously.

As a consequence, our basic parts are mainly selected based on the relationship between Saccharomyces cerevisiae and Yarrowia lipolytica.

Basic Parts Table

Name Type Description
BBa_K3112000 DNA S.C cenXV
BBa_K3112001 DNA Y.L cenE
BBa_K3112002 Coding dCas9
BBa_K3112004 Regulatory TEF promoter
BBa_K3112005 Terminator CYC1 Terminator
BBa_K3112007 RNA gRNA
BBa_K3112008 DNA Protospacer+PAM
BBa_K3112009 Coding GFP
BBa_K3112010 Coding mRFP use in yeast
BBa_K3112011 Regulatory PGK1 Promoter
BBa_K3112012 Terminator PGK1 Terminator
BBa_K3112013 Coding yEGFP
BBa_K3112014 Regulatory His3 Promoter
BBa_K3112015 Coding His3 Gene
BBa_K3112016 Regulatory LEU2 Promoter
BBa_K3112017 Coding LEU2 Gene
BBa_K3112018 Regulatory URA3 Promoter
BBa_K3112019 Coding URA3 Gene
BBa_K3112020 Coding Hygromycin Resistance Gene(hyg or hph)
BBa_K3112021 Coding A to G adenine Base Editor
BBa_K3112022 Terminator TEF Terminator
BBa_K3112023 Coding APOBEC1 cytidine deaminase
BBa_K3112024 Regulatory GAP Promoter
BBa_K3112025 Terminator ADH1 Terminator
BBa_K3112026 DNA Homologous arm in Yarrowia lipolytica E Chromosome 1
BBa_K3112027 DNA Homologous arm in Yarrowia lipolytica E Chromosome 2
BBa_K3112028 DNA Homologous arm in Saccharomyces cerevisiae V Chromosome 1
BBa_K3112029 DNA Homologous arm in Saccharomyces cerevisiae V Chromosome 2
BBa_K3112030 DNA Homologous arm in Saccharomyces cerevisiae V Chromosome 3
BBa_K3112031 DNA Homologous arm in Saccharomyces cerevisiae V Chromosome 4
BBa_K3112032 DNA Left Homologous Arm for Homologous Recombination of Saccharomyces cerevisiae
BBa_K3112033 DNA Right Homologous Arm for Homologous Recombination of Saccharomyces cerevisiae
BBa_K3112034 DNA Left Homologous Arm for Homologous Recombination of Yarrowia lipolytica
BBa_K3112035 DNA Right Homologous Arm for Homologous Recombination of Yarrowia lipolytica
BBa_K3112037 Coding KanMX