Team:Tianjin/Notebook

Notebook

Protocol

Notebook

Reference

Sequence supportment: NCBI

We used the protocol of the paper below to fuse S. cerevisiae and S.pombe, and we have made some optimize.

[1]Gao Nianfa, Wang Shuhao, Li Xiaogang, Protoplast fusion between Saccharomyces cerevisiae and Schizosaccharomyces cerevisiae to isolate wine yeast with strong malic acid degradation[J].Chinese Journal of Biotechnology,2000,16(6):1- 5.

We used the protocol of the paper below to fuse S. cerevisiae and Y. lipolytica and we have made some optimize.

[2]Sipiczki M , Ferenczy L . Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type[J]. MGG - Molecular and General Genetics, 1977, 151(1):77-81.

We extracted the method of S. cerevisiae protoplast preparation and optimized and rectified it.

[3]Zhang Borun, Wang Yonghong, Liu Shufeng, et al. Fusion of Saccharomyces cerevisiae protoplasts[J]. Bulletin of Microbiology, 1986(2

We extracted the method for preparing Y. lipolytica protoplasts and we make the optimization of the protocol.

[4]Hou Xiaojiao, Xu Kun. Preparation and regeneration conditions of protoplasts of Yarrowia lipolytica[J]. Journal of Liaodong University (Natural Science Edition), 2019, 26(01): 47-53.