MoClo
Date | What? | Construct |
1/30/2019 | ligation | L2A |
2/21/2019 | ligation | L2C |
3/18/2019 | ligation | L2E |
4/9/2019 | ligation | L2K |
4/10/2019 | ligation | L2H |
4/29/2019 | ligation | L2M |
6/26/2019 | ligation | L2Q |
7/1/2019 | ligation | L2S |
8/5/2019 | ligation | L2V |
8/13/2019 | ligation | L2AC |
8/22/2019 | ligation | L2AD |
Screening
3/14/2019 | expression of PETase and MHETase |
3/22/2019 | secretion of MHETase (L2C) |
4/17/2019 | localization of PETase in ER |
6/6/2019 | secretion of PETase (L2J) |
6/12/2019 | secretion of both, MHETase and PETase (SP20-tag) |
6/19/2019 | identification of MHETase and PETase (MS) |
8/14/2019 | quantifiying yield of secretion |
8/21/2019 | secretion of MHETase and PETase of clip transformants |
9/24/2019 | analysis of linkage between different growth conditions and secretion efficiency |
Chlamy Physiology
3/16/2019 | attachment of UVM4 onto PET |
5/06/2019- 05/10/2019 | toxicity test |
7/25/2019 | attachment of Clip onto PET |
9/15/2019 | spot test |
10/1/2019-10/7/2019 | auxotrophy test |
Analytics
5/10/2019 | start of analytical experiments |
5/29/2019 | first HPLC purification |
6/6/2019 | first HPLC measurement |
6/18/2019 | successfull cloning from PETase at pet21b |
7/8/2019 | microscopy of PET |
7/25/2019 | purification with magneticbeats |
8/20/2019 | HPLC measurement |
8/23/2019 | successfull His-tag purifikation |
9/30/2019 | HPLC measurement |
9/30 - 10/7/2019 | last HPLC measurement |
Sponsoring
2/5/2019 | Qiagen agreed to sponsor us |
2/6/2019 | ZRW agreed to sponsor us starting april the 16th 2019 with the first status report |
2/11/2019 | ROTH agreed to sponsor us |
2/18/2019 | Jena Bioscience agreed to sponsor us |
3/21/2019 | Promega agreed to sponsor us |
3/27/2019 | New england biolabs agreed to sponsor us |
4/29/2019 | SWK agreed to sponsor us |
5/5/2019 | Implen agreed to sponsor us |
6/12/2019 | Biocomp agreed to sponsor us |
6/18/2019 | meeting Alpla at their Company |
6/26/2019 | LTF agreed to sponsor us |
7/16/2019 | meeting Mitsubishi in our laboratory |
8/7/2019 | meeting with the „Mainzer Wissenschafts Stiftung" |
Crowdfunding
1/15/2019 | idea to start a Crowdfunding campaign |
2/7/2019 | first meeting with the "Gründungsbüro" of our university |
3/2/2019 | first plans for the campaign |
5/8/2019 | workshop at the "center for business and innovation" about |
5/17/2019 | status report and troubleshooting at the "Gründungsbüro" |
6/17/2019 | start of the feedback phase |
6/24 - 7/31/2019 | financing phase |
8/20 - 10/10/2019 | realization phase |
Conferences
5/3 -5/5/2019 | iGEM Spring Festival Bonn, Germany |
6/5 - 6/7/2019 | iGEM German Meetup Düsseldorf, Germany |
7/25 - 7/27/2019 | iGEM european Meetup Paris, France |
9/12 - 9/13/2019 | German Conference on Synthetic Biology Aachen, Germany |
9/20 - 9/22/2019 | Synbio gone viral Meetup Leuven, Belgium |
Public Relations
4/7 - 10/4/2019 | survey |
4/10/2019 | sweep-week in cooperation with SWK in Kaiserslautern |
5/16/2019 | workshop for intellectual property "Schutz von Innovationen" |
5/20 - 9/30/2019 | art competition"Studieninformationstag" at our University to inform students about iGEM |
5/21/2019 | "Studieninformationstag" at our University to inform students about iGEM |
5/22/2019 | visiting "Albert Schweizer" high school Kaiserslautern |
5/26/2019 | visit at the gardening exhibition |
5/30/2019 | participating at the sustainability week at our University |
6/3/2019 | Biological Colloquium with Professor Zimmermann |
6/4/2019 | visiting a lection from Prof. Schroda to inform about iGEM |
6/5/2019 | visiting "Kusel" high school |
6/6/2019 | visiting "Rittersberg" high school Kaiserslautern |
6/7/2019 | visiting "Landstuhl Sicking"-high school |
6/11/2019 | crêpe sale at our University |
6/12/2019 | visiting a lection from Prof. Frankenberg-Dinkel to inform about iGEM |
6/12/2019 | visiting lection from Prof. Frankenberg-Dinkel to inform about iGEM |
6/24/2019 | visiting the "Burg" - high school Kaiserslautern |
6/25/2019 | visiting the radio station "Antenne Kaiserslautern" |
6/25/2019 | info-meeting about iGEM for the next iGEM-team |
7/20/2019 | packaging in the supermarket "Wasgau" |
7/30/2019 | ethics panel discussion |
8/8/2019 | meeting with the politician Mr.Rahm |
8/20/2019 | SWR radio visited our laboratory |
8/29/2019 | a day in the lab with handicapped people |
9/30/2019 | student internship in our lab |
10/4/2019 | visiting the exhibition for senior citiziens |
10/10/2019 | visiting the local sewage plant |