Team:Stanford/Part Collection
Parts Collection
Parts Collection
| Part | Name | |
|---|---|---|
| 1 | BBa_K3258001 | M13 pIV |
| 2 | BBa_K3258003 | M13 pII |
| 3 | BBa_K3258004 | M13 pIX |
| 4 | BBa_K3258005 | M13 pVII |
| 5 | BBa_K3258006 | Infection Reporter (Design Using BBa_K2832003) |
| 6 | BBa_K3258007 | M13 pIII Neg |
| 7 | BBa_K3258008 | M13 Helper Plasmid for PREDCEL+ |
| 8 | BBa_K3258009 | Selection Plasmid PREDCEL+ |
| 9 | BBa_K3258010 | T7 to T3 Selection Schema PREDCEL+ |
| 10 | BBa_K3258011 | lacI-derived biosensor selection schema (positive selection) PREDCEL+ |
| 11 | BBa_K3258012 | lacI-derived biosensor selection schema (negative selection) PREDCEL+ |
| 12 | BBa_K3258013 | Selection Plasmid T7 to T3 PREDCEL+ |
| 13 | BBa_K3258014 | Selection Plasmid T7 to T3 PREDCEL+ (Alternate Version) |
| 14 | BBa_K3258019 | M13 pVI |
Best Parts Collection
We submit PREDCEL+ SHOP as our best new parts collection. SHOP stands for Selection Helper and Other Plasmids, and is our collection of components to assist in the implementation of selection schema and directed evolution using our improved PREDCEL+ directed evolution technique. To make PREDCEL more accessible to iGEM teams, we created a standard set of three plasmids to implement user-defined selection schema. It improves PREDCEL in three ways. First, our selection is based off pVI instead of pIII expression - significant because pIII renders cells immune to infection. Second, our plasmid architecture enables ‘infection reporter’ use, which provides a visual indicator of the number of cells infected in a given E. coli population. This feedback is used to modulate selection stringency, driving evolution forward. Finally, pIII expression is triggered post-infection, preventing multiple phage phenotypes from infecting a cell. This stops “cheater” phenotypes from proliferating as described by Brodel et al. (2016). To document this collection, we present all M13 phage genes not previously documented alongside composites we used for evolving T7 polymerase to recognize a T3 promoter and new lacI based biosensors.
