Team:Shanghai HS United/Parts
We used a synthetic DNA fragment to simulate African swine fever virus (ASFv) DNA, and the DNA we adopted CAN NOT express any proteins. The DNA sequence is same as a part of b464 gene within ASFv genome. We use the sequence to test our detection method, which may be used as a new rapid detection method for ASFv.
The b464 gene is a coding sequence of the major coat protein vp72 which is essential for ASFv. So we used the synthetic fragment to mimic the ASFv DNA for testing our new detection method.
It is a J23100 promoter, and it is constructed in a typeIIS plasmid. It is a synthetic sequence. We constuct the part as a promoter in typeIIS standard plasmid.
It is a B0034 RBS, and it is constructed in a typeIIS plasmid. It is a synthetic sequence. We constuct the part as an RBS in typeIIS standard plasmid.
It is a conding sequence of CRISPR-Cas12a from Francisella novicida U112. The sequence is coden optimized. We constuct the part as a coding sequence in typeIIS standard plasmid.
It is a terminator which is constucted as the part in typeIIS standard plasmid.
It is a conding sequence of GPF which is constucted the part as a coding sequence in typeIIS standard plasmid.
It is a conding sequence of GPF which is constucted the part as a coding sequence in typeIIS standard plasmid.
It is a composite part which can expression GFP protein using typeIIS standard assembly. We use it as a reporter system to test the protein expression in typeIIS scars. Becase we know that the scar between RBS and coding sequence would effect the protein expression.
BBa_K3136002, BBa_K3136003, BBa_K3136005, BBa_K3136006
It is a reporter sequence for blue-white screening.If other DNA sequence inserts into this place, the clony will become white, otherwise it is blue. It is PCR amplified from the plasmid pUC18.
It is a plasmid backbone which is derived from pSB1K3 for typeIIS assembly.
It is a mutation sequence from B0034 RBS, and it is constructed in a typeIIS plasmid. We know that the scar between RBS and coding sequence would effect the protein expression, so we construct several different RBSs to test the expression strength.
It is a mutation sequence from B0034 RBS, and it is constructed in a typeIIS plasmid. We know that the scar between RBS and coding sequence would effect the protein expression, so we construct several different RBSs to test the expression strength.
It is a mutation sequence from B0034 RBS, and it is constructed in a typeIIS plasmid. We know that the scar between RBS and coding sequence would effect the protein expression, so we construct several different RBSs to test the expression strength.
It is a mutation sequence from B0034 RBS, and it is constructed in a typeIIS plasmid. We know that the scar between RBS and coding sequence would effect the protein expression, so we construct several different RBSs to test the expression strength.
These RBS sequences are a little differnece to BBa_0034. As the sacr between BioBrick RFC_10 and typeIIS assembly are different and may effect the protein expression, we constructed the four RBS to measure the expression strength.
These sequences are template of crRNA which used to transcript into ASFv DNA (b464 gene) targeted crRNA sequence in vitro.